Isolation, characterization, and purification of macrophages from tissues affected by obesity-related inflammation

Journal of Visualized Experiments

Volumn 2017, Issue 122, 2017, Pages

  Allen, Joselyn N ^a   Dey, Adwitia ^a   Nissly, Ruth ^b   Fraser, James ^a   Yu, Shan ^a   Balandaram, Gayathri ^a   Peters, Jeffrey M ^a   Hankey Giblin, Pamela A ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Adipose tissue digestion; Aorta digestion; Cell sorting; Diet induced inflammation; Enzymatic tissue digestion; FACS; Immunology; Issue 122; Liver perfusion; Macrophages; Monoclonal antibodies; Obesity; Single cell suspension

Indexed keywords

AUTACOID;

ANIMAL; C57BL MOUSE; CHOLESTEROL INTAKE; CYTOLOGY; INFLAMMATION; LIPID DIET; MACROPHAGE; MALE; MOUSE; OBESITY; PATHOLOGY; PHYSIOLOGY; WHITE ADIPOSE TISSUE;

ADIPOSE TISSUE, WHITE; ANIMALS; CHOLESTEROL, DIETARY; DIET, HIGH-FAT; INFLAMMATION; INFLAMMATION MEDIATORS; MACROPHAGES; MALE; MICE; MICE, INBRED C57BL; OBESITY;

EID: 85017579790     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/55445     Document Type: Article

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