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Volumn , Issue , 2003, Pages 225-233

Meaningful quantification of mRNA using real-time PCR

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EID: 85014762533     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter
Times cited : (7)

References (39)
  • 1
    • 0031841991 scopus 로고    scopus 로고
    • Developments in quantitative PCR
    • Orlando, C. et al., Developments in quantitative PCR, Clin. Chem. Lab. Med., 36, 255, 1998.
    • (1998) Clin. Chem. Lab. Med. , vol.36 , pp. 255
    • Orlando, C.1
  • 2
    • 0029957181 scopus 로고    scopus 로고
    • Real time quantitative PCR
    • Heid, C. A. et al., Real time quantitative PCR, Genome Res., 6, 986, 1996.
    • (1996) Genome Res. , vol.6 , pp. 986
    • Heid, C.A.1
  • 3
    • 0037085729 scopus 로고    scopus 로고
    • Real-time PCR in virology
    • Mackay, I. M. et al., Real-time PCR in virology, Nucleic Acids Res., 30, 1292, 2002.
    • (2002) Nucleic Acids Res. , vol.30 , pp. 1292
    • Mackay, I.M.1
  • 4
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin, S. A., Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays, J. Mol. Endocrinol., 25, 169, 2000.
    • (2000) J. Mol. Endocrinol. , vol.25 , pp. 169
    • Bustin, S.A.1
  • 5
    • 0032894224 scopus 로고    scopus 로고
    • Quantitative RT-PCR, pitfalls and potential
    • Freeman, W. M. et al., Quantitative RT-PCR, pitfalls and potential, BioTechniques, 26, 112, 1999.
    • (1999) BioTechniques , vol.26 , pp. 112
    • Freeman, W.M.1
  • 6
    • 0034840021 scopus 로고    scopus 로고
    • Expression of HLA class II in colorectal cancer, evidence for enhanced immunogenicity of microsatellite-instability-positive tumours
    • Bustin, S. A. et al., Expression of HLA class II in colorectal cancer, evidence for enhanced immunogenicity of microsatellite-instability-positive tumours, Tumour Biol., 22, 294, 2001.
    • (2001) Tumour Biol. , vol.22 , pp. 294
    • Bustin, S.A.1
  • 7
    • 10544229339 scopus 로고    scopus 로고
    • Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
    • Schuurman, R. et al., Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma, J. Clin. Microbiol., 34, 3016, 1996.
    • (1996) J. Clin. Microbiol. , vol.34 , pp. 3016
    • Schuurman, R.1
  • 8
    • 15744381713 scopus 로고
    • Kinetic PCR analysis, real-time monitoring of DNA amplification reactions
    • Higuchi, R. et al., Kinetic PCR analysis, real-time monitoring of DNA amplification reactions, Biotechnology (NY), 11, 1026, 1993.
    • (1993) Biotechnology (NY) , vol.11 , pp. 1026
    • Higuchi, R.1
  • 9
    • 0032883806 scopus 로고    scopus 로고
    • Housekeeping genes as internal standards, use and limits
    • Thellin, O. et al., Housekeeping genes as internal standards, use and limits, J. Biotechnol., 75, 291, 1999.
    • (1999) J. Biotechnol. , vol.75 , pp. 291
    • Thellin, O.1
  • 10
    • 0032247960 scopus 로고    scopus 로고
    • Quantification of mRNA by polymerase chain reaction (PCR) using an internal standard and a nonradioactive detection method
    • Karge, W. H. et al., Quantification of mRNA by polymerase chain reaction (PCR) using an internal standard and a nonradioactive detection method, Methods Mol. Biol., 110, 43, 1998.
    • (1998) Methods Mol. Biol. , vol.110 , pp. 43
    • Karge, W.H.1
  • 11
    • 0031916683 scopus 로고    scopus 로고
    • Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays
    • Haberhausen, G. et al., Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays, J. Clin. Microbiol., 36, 628, 1998.
    • (1998) J. Clin. Microbiol. , vol.36 , pp. 628
    • Haberhausen, G.1
  • 12
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RT-PCR, trends and problems
    • Bustin, S. A., Quantification of mRNA using real-time RT-PCR, trends and problems, J. Mol. Endo- crinol., 29, 23, 2002.
    • (2002) J. Mol. Endo- crinol. , vol.29 , pp. 23
    • Bustin, S.A.1
  • 13
    • 0028125811 scopus 로고
    • Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28S-ribosomal RNA gene expression as RNA loading controls for northern blot analysis of cell lines of varying malignant potential
    • Bhatia, P. et al., Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28S-ribosomal RNA gene expression as RNA loading controls for northern blot analysis of cell lines of varying malignant potential, Anal. Biochem., 216, 223, 1994.
    • (1994) Anal. Biochem. , vol.216 , pp. 223
    • Bhatia, P.1
  • 14
    • 0033575003 scopus 로고    scopus 로고
    • Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia
    • Zhong, H. and Simons, J. W., Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia, Biochem. Biophys. Res. Commun., 259, 523, 1999.
    • (1999) Biochem. Biophys. Res. Commun. , vol.259 , pp. 523
    • Zhong, H.1    Simons, J.W.2
  • 15
    • 0034694128 scopus 로고    scopus 로고
    • Effect of experimental treatment on housekeeping gene expression, validation by real-time, quantitative RT-PCR
    • Schmittgen, T. D. and Zakrajsek, B. A., Effect of experimental treatment on housekeeping gene expression, validation by real-time, quantitative RT-PCR, J. Biochem. Biophys. Methods, 46, 69, 2000.
    • (2000) J. Biochem. Biophys. Methods , vol.46 , pp. 69
    • Schmittgen, T.D.1    Zakrajsek, B.A.2
  • 16
    • 0035423813 scopus 로고    scopus 로고
    • Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations
    • Goidin, D. et al., Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations, Anal. Biochem., 295, 17, 2001.
    • (2001) Anal. Biochem. , vol.295 , pp. 17
    • Goidin, D.1
  • 17
    • 0035161457 scopus 로고    scopus 로고
    • Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors
    • Solanas, M. et al., Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors, Anal. Biochem., 288, 99, 2001.
    • (2001) Anal. Biochem. , vol.288 , pp. 99
    • Solanas, M.1
  • 18
    • 0032534813 scopus 로고    scopus 로고
    • RNA quantitation by fluorescence-based solution assay, RiboGreen reagent characterization
    • Jones, L. J. et al., RNA quantitation by fluorescence-based solution assay, RiboGreen reagent characterization, Anal. Biochem., 265, 368, 1998.
    • (1998) Anal. Biochem. , vol.265 , pp. 368
    • Jones, L.J.1
  • 19
    • 0029739644 scopus 로고    scopus 로고
    • Quantitative RT-PCR, limits and accuracy
    • Souaze, F. et al., Quantitative RT-PCR, limits and accuracy, BioTechniques, 21, 280, 1996.
    • (1996) BioTechniques , vol.21 , pp. 280
    • Souaze, F.1
  • 20
    • 0032170539 scopus 로고    scopus 로고
    • Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards
    • Gerard, C. J. et al., Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards, Cancer Res., 58, 3957, 1998.
    • (1998) Cancer Res. , vol.58 , pp. 3957
    • Gerard, C.J.1
  • 21
    • 0030614426 scopus 로고    scopus 로고
    • The LightCycler, a microvolume multisample fluorimeter with rapid temperature control
    • Wittwer, C. T. et al., The LightCycler, a microvolume multisample fluorimeter with rapid temperature control, BioTechniques, 22, 176, 1997.
    • (1997) BioTechniques , vol.22 , pp. 176
    • Wittwer, C.T.1
  • 22
    • 0031059823 scopus 로고    scopus 로고
    • Two variants of quantitative reverse transcriptase PCR used to show differential expression of alpha-, beta- and gamma-fibrinogen genes in rat liver lobes
    • Zhang, J. et al., Two variants of quantitative reverse transcriptase PCR used to show differential expression of alpha-, beta- and gamma-fibrinogen genes in rat liver lobes, Biochem. J., 321, 769, 1997.
    • (1997) Biochem. J. , vol.321 , pp. 769
    • Zhang, J.1
  • 23
    • 0032896433 scopus 로고    scopus 로고
    • Reproducibility of detection of tyrosinase and MART-1 transcripts in the peripheral blood of melanoma patients, a quality control study using real-time quantitative RT-PCR
    • de Vries, T. J. et al., Reproducibility of detection of tyrosinase and MART-1 transcripts in the peripheral blood of melanoma patients, a quality control study using real-time quantitative RT-PCR, Br. J. Cancer, 80, 883, 1999.
    • (1999) Br. J. Cancer , vol.80 , pp. 883
    • de Vries, T.J.1
  • 24
    • 0029935969 scopus 로고    scopus 로고
    • Theoretical uncertainty of measurements using quantitative polymerase chain reaction
    • Peccoud, J. and Jacob, C., Theoretical uncertainty of measurements using quantitative polymerase chain reaction, Biophys. J., 71, 101, 1996.
    • (1996) Biophys. J. , vol.71 , pp. 101
    • Peccoud, J.1    Jacob, C.2
  • 25
    • 0030461224 scopus 로고    scopus 로고
    • Loss of heterozygosity in normal tissue adjacent to breast carcinomas
    • Deng, G. et al., Loss of heterozygosity in normal tissue adjacent to breast carcinomas, Science, 274, 2057, 1996.
    • (1996) Science , vol.274 , pp. 2057
    • Deng, G.1
  • 26
    • 0000511029 scopus 로고
    • Illegitimate transcription, transcription of any gene in any cell type
    • Chelly, J. et al., Illegitimate transcription, transcription of any gene in any cell type, Proc. Natl. Acad. Sci. U.S.A., 86, 2617, 1989.
    • (1989) Proc. Natl. Acad. Sci. U.S.A. , vol.86 , pp. 2617
    • Chelly, J.1
  • 27
    • 0036141101 scopus 로고    scopus 로고
    • cDNA array hybridization after laser-assisted microdissection from nonneoplastic tissue
    • Fink, L. et al., cDNA array hybridization after laser-assisted microdissection from nonneoplastic tissue, Am. J. Pathol., 160, 81, 2002.
    • (2002) Am. J. Pathol. , vol.160 , pp. 81
    • Fink, L.1
  • 28
    • 0036141385 scopus 로고    scopus 로고
    • Microdissection is essential for gene expression profiling of clinically resected cancer tissues
    • Sugiyama, Y. et al., Microdissection is essential for gene expression profiling of clinically resected cancer tissues, Am. J. Clin. Pathol., 117, 109, 2002.
    • (2002) Am. J. Clin. Pathol. , vol.117 , pp. 109
    • Sugiyama, Y.1
  • 29
    • 0030575911 scopus 로고    scopus 로고
    • Laser capture microdissection
    • Emmert-Buck, M. R. et al., Laser capture microdissection, Science, 274, 998, 1996.
    • (1996) Science , vol.274 , pp. 998
    • Emmert-Buck, M.R.1
  • 30
    • 0035046687 scopus 로고    scopus 로고
    • Tissue microdissection techniques in quantitative genome and gene expression analyses
    • Walch, A. et al., Tissue microdissection techniques in quantitative genome and gene expression analyses, Histochem. Cell Biol., 115, 269, 2001.
    • (2001) Histochem. Cell Biol. , vol.115 , pp. 269
    • Walch, A.1
  • 31
    • 0034460871 scopus 로고    scopus 로고
    • Cell type-specific mRNA quantitation in non-neoplastic tissues after laser-assisted cell picking
    • Bohle, R. M. et al., Cell type-specific mRNA quantitation in non-neoplastic tissues after laser-assisted cell picking, Pathobiology, 68, 191, 2000.
    • (2000) Pathobiology , vol.68 , pp. 191
    • Bohle, R.M.1
  • 32
    • 0034982548 scopus 로고    scopus 로고
    • Small-sample total RNA purification, laser capture microdissection and cultured cell applications
    • Dolter, K. E. and Braman, J. C., Small-sample total RNA purification, laser capture microdissection and cultured cell applications, BioTechniques, 30, 1358, 2001.
    • (2001) BioTechniques , vol.30 , pp. 1358
    • Dolter, K.E.1    Braman, J.C.2
  • 33
    • 0035423799 scopus 로고    scopus 로고
    • One-step extraction of RNA from archival biopsies
    • Bock, O. et al., One-step extraction of RNA from archival biopsies, Anal. Biochem., 295, 116, 2001.
    • (2001) Anal. Biochem. , vol.295 , pp. 116
    • Bock, O.1
  • 34
    • 0034189805 scopus 로고    scopus 로고
    • Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction
    • Godfrey, T. E. et al., Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction, J. Mol. Diagn., 2, 84, 2000.
    • (2000) J. Mol. Diagn. , vol.2 , pp. 84
    • Godfrey, T.E.1
  • 35
    • 0035134463 scopus 로고    scopus 로고
    • Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue
    • Specht, K. et al., Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue, Am. J. Pathol., 158, 419, 2001.
    • (2001) Am. J. Pathol. , vol.158 , pp. 419
    • Specht, K.1
  • 36
    • 0036149243 scopus 로고    scopus 로고
    • Laser microdissection and gene expression analysis on formaldehyde-fixed archival tissue
    • Cohen, C. D. et al., Laser microdissection and gene expression analysis on formaldehyde-fixed archival tissue, Kidney Int., 61, 125, 2002.
    • (2002) Kidney Int. , vol.61 , pp. 125
    • Cohen, C.D.1
  • 37
    • 0034106072 scopus 로고    scopus 로고
    • Immunostaining and laser-assisted cell picking for mRNA analysis
    • Fink, L. et al., Immunostaining and laser-assisted cell picking for mRNA analysis, Lab. Invest., 80, 327, 2000.
    • (2000) Lab. Invest. , vol.80 , pp. 327
    • Fink, L.1
  • 38
    • 0033785728 scopus 로고    scopus 로고
    • Increased sensitivity of one-tube, quantitative RT-PCR
    • Raja, S. et al., Increased sensitivity of one-tube, quantitative RT-PCR, BioTechniques, 29, 702, 2000.
    • (2000) BioTechniques , vol.29 , pp. 702
    • Raja, S.1
  • 39
    • 0035021256 scopus 로고    scopus 로고
    • Thermal effects on reverse transcription, improvement of accuracy and processivity in cDNA synthesis
    • Malboeuf, C. M. et al., Thermal effects on reverse transcription, improvement of accuracy and processivity in cDNA synthesis, BioTechniques, 30, 1074, 2001.
    • (2001) BioTechniques , vol.30 , pp. 1074
    • Malboeuf, C.M.1


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