Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor

World Journal of Gastroenterology

Volumn 23, Issue 6, 2017, Pages 964-975

  Han, Sung Hoon ^a   Shim, Sehwan ^a   Kim, Min Jung ^a   Shin, Hye Yun ^a   Jang, Won Suk ^a   Lee, Sun Joo ^a   Jin, Young Woo ^a   Lee, Seung Sook ^a   Lee, Seung Bum ^a   Park, Sunhoo ^a  

^a Korea Institute of Radiation and Medical Science   (South Korea)

Author keywords

Cryopreservation; Intestinal organoid; Longterm culture; Rho kinase inhibitor; Three dimensional culture

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; EPIDERMAL GROWTH FACTOR; NOGGIN; RHO KINASE INHIBITOR; AMIDE; ENZYME INHIBITOR; PYRIDINE DERIVATIVE; RHO KINASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; COMPARATIVE STUDY; CONTROLLED STUDY; CRYOPRESERVATION; CRYPT CELL; FREEZE THAWING; IMMUNOFLUORESCENCE; IN VITRO STUDY; INTESTINE CRYPT; INTESTINE EPITHELIUM CELL; JEJUNUM; MALE; METHYL THIAZOLYL TETRAZOLIUM ASSAY; MOUSE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; SMALL INTESTINAL ORGANOID; SMALL INTESTINE DISEASE; TIME LAPSE IMAGING; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOLOGICAL THERAPY; C57BL MOUSE; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL DIFFERENTIATION; CYTOLOGY; CYTOPLASM; INTESTINE MUCOSA; PHYSIOLOGY; PROCEDURES; SMALL INTESTINE; TIME FACTOR;

AMIDES; ANIMALS; BIOLOGICAL THERAPY; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; CRYOPRESERVATION; ENZYME INHIBITORS; INTESTINAL MUCOSA; INTESTINE, SMALL; MALE; MICE; MICE, INBRED C57BL; ORGANOIDS; PYRIDINES; RHO-ASSOCIATED KINASES; TIME FACTORS;

EID: 85014513807     PISSN: 10079327     EISSN: 22192840     Source Type: Journal    
DOI: 10.3748/wjg.v23.i6.964     Document Type: Article

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