Knocking out consumer concerns and regulator's rules: Efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

Genome Biology

Volumn 18, Issue 1, 2017, Pages

  Wolter, Felix ^a   Puchta, Holger ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 RIBONUCLEOPROTEIN; RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG;

CEREAL; CONSUMER ATTITUDE; CRISPR CAS SYSTEM; GENE EDITING; MAIZE; NONHUMAN; NOTE; PLANT GENE; WHEAT; FOOD GRAIN; GENE TARGETING; GENETICS; METABOLISM; PLANT GENOME;

CRISPR-CAS SYSTEMS; EDIBLE GRAIN; GENE EDITING; GENE TARGETING; GENOME, PLANT; RIBONUCLEOPROTEINS;

EID: 85014106736     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-017-1179-1     Document Type: Note

References (9)

1. Svitashev S, Schwartz C, Lenderts B, Young JK, Mark Cigan A. Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes. Nat Commun. 2016;7:13274. doi: 10.1038/ncomms13274.
2. Liang Z, Chen K, Li T, Zhang Y, Wang Y, Zhao Q, et al. Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nat Commun. 2017;8:14261. doi: 10.1038/ncomms14261.
3. Schiml S, Puchta H. Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas. Plant Methods. 2016;12:8. doi: 10.1186/s13007-016-0103-0.
4. Hilscher J, Burstmayr H, Stoger E. Targeted modification of plant genomes for precision crop breeding. Biotechnol J. 2017. doi: 10.1002/biot.201600173.
5. Pacher M, Puchta H. From classical mutagenesis to nuclease-based breeding--directing natural DNA repair for a natural end-product. Plant J. 2016. doi: 10.1111/tpj.13469.
6. Kim S, Kim D, Cho SW, Kim J, Kim J-S. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 2014;24:1012-9. doi: 10.1101/gr.171322.113.
7. Woo JW, Kim J, Kwon SI, Corvalan C, Cho SW, Kim H, et al. DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins. Nat Biotechnol. 2015;33:1162-4. doi: 10.1038/nbt.3389.
8. Zhang Y, Liang Z, Zong Y, Wang Y, Liu J, Chen K, et al. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nat Commun. 2016;7:12617. doi: 10.1038/ncomms12617.
9. Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol. 2014;32:947-51. doi: 10.1038/nbt.2969.