Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Nature Communications

Volumn 7, Issue , 2016, Pages

  Zhang, Yi ^a,b   Liang, Zhen ^a,b   Zong, Yuan ^a,b   Wang, Yanpeng ^a,b   Liu, Jinxing ^a   Chen, Kunling ^a   Qiu, Jin Long ^a   Gao, Caixia ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GUIDE RNA; PLANT DNA; PLANT RNA; RNA;

BIOENGINEERING; CELLS AND CELL COMPONENTS; DNA; GENE EXPRESSION; GENOME; MUTATION; RESEARCH WORK; RNA; WHEAT;

ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; FRAMESHIFT MUTATION; GENETIC PROCEDURES; HEXAPLOIDY; NONHUMAN; PLANT CELL; PLANT GENE; PLANT GENOME; TECCDNA GENOME EDITING METHOD; TECCRNA GENOME EDITING METHOD; TETRAPLOIDY; TRANSGENE; TRANSIENT EXPRESSION; TRITICUM AESTIVUM; TRITICUM DURUM; EVALUATION STUDY; GENE EDITING; GENETICS; HOMOZYGOTE; LEGISLATION AND JURISPRUDENCE; MUTATION; POLYPLOIDY; PROCEDURES; TRANSGENIC PLANT; WHEAT;

TRITICUM AESTIVUM; TRITICUM TURGIDUM SUBSP. DURUM;

CRISPR-CAS SYSTEMS; DNA; GENE EDITING; GENES, PLANT; HOMOZYGOTE; MUTATION; PLANTS, GENETICALLY MODIFIED; POLYPLOIDY; RNA; TRITICUM;

EID: 84983783288     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms12617     Document Type: Article

References (28)

1. Voytas, D. F., Gao, C. Precision genome engineering and agriculture: opportunities and regulatory challenges. PLoS Biol. 12, 429-440 (2014).
2. Gao, C. Genome editing in crops: from bench to field. Natl. Sci. Rev. 2, 13-15 (2015).
3. Symington, L. S., Gautier, J. Double-strand break end resection and repair pathway choice. Annu. Rev. Genet. 45, 247-271 (2011).
4. Lawrenson, T. et al. Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 16, 258 (2015).
5. Jones, H. D. Regulatory uncertainty over genome editing. Nat. Plants 1, 14011 (2015).
6. Woo, J. et al. DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins. Nat. Biotechnol. 33, 1162-1164 (2015).
7. Ling, H. et al. Draft genome of the wheat A-genome progenitor Triticum urartu. Nature 496, 87-90 (2013).
8. Shan, Q., Wang, Y., Li, J., Gao, C. Genome editing in rice and wheat using the CRISPR/Cas system. Nat. Protoc. 9, 2395-2410 (2014).
9. Wang, Y. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat. Biotechnol. 32, 947-951 (2014).
10. Huang, X. et al. Natural variation at the DEP1 locus enhances grain yield in rice. Nat. Genet. 41, 494-497 (2009).
11. Mao, C. et al. Overexpression of a NAC-domain protein promotes shoot branching in rice. New Phytol. 176, 288-298 (2007).
12. Xu, M., Zhu, L., Shou, H., Wu, P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant Cell Physiol. 46, 1674-1681 (2005).
13. Feng, B. et al. Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. J. Cereal Sci. 52, 387-394 (2010).
14. Yang, Z. et al. SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight. Theor. Appl Genet. 125, 1057-1068 (2012).
15. Xiao, A. et al. CasOT: a genome-wide Cas9/gRNA off-target searching tool. Bioinformatics 30, 1180-1182 (2014).
16. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).
17. Pattanayak, V. et al. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol. 31, 839-843 (2013).
18. Hsu, P. D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832 (2013).
19. Dong, L. et al. Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions. Mol. Breeding 34, 937-947 (2014).
20. Kim, S., Kim, D., Cho, S. W., Kim, J., Kim, J.-S. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24, 1012-1019 (2014).
21. Ramakrishna, S. et al. Gene disruption by cell-penetrating peptidemediated delivery of Cas9 protein and guide RNA. Genome Res. 24, 1020-1027 (2014).
22. Zuris, J. A. et al. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat. Biotechnol. 33, 73-80 (2015).
23. Cutler, A. J., Saleem, M., Wang, H. Cereal protoplast recalcitrance. In Vitro Cell. Dev. Boil. 27, 104-111 (1991).
24. Xu, R.-F. et al. Generation of inheritable and 'transgene clean' targeted genome-modified rice in later generations using the CRISPR/Cas9 system. Sci. Rep. 5, 11491 (2015).
25. Jacobs, T. B., LaFayette, P. R., Schmitz, R. J., Parrott, W. A. Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnol. 15, 16 (2015).
26. Shan, Q. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat. Biotechnol. 31, 686-688 (2013).
27. Rasco-Gaunt, S. et al. Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. J. Exp. Bot. 52, 865-874 (2001).
28. Christensen, A., Quail, P. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5, 213-218 (1996).