Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Nature Communications

Volumn 8, Issue , 2017, Pages

  Liang, Zhen ^a,b   Chen, Kunling ^a   Li, Tingdong ^a,b   Zhang, Yi ^a,b   Wang, Yanpeng ^a,b   Zhao, Qian ^a,b   Liu, Jinxing ^a   Zhang, Huawei ^a   Liu, Cuimin ^a   Ran, Yidong ^c   Gao, Caixia ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Genovo Biotechnology Co Ltd   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PLANT DNA; RIBONUCLEOPROTEIN;

CROP PLANT; DNA; GENE EXPRESSION; GENOME; MUTATION; OPTIMIZATION; PROTEIN; SELECTIVE BREEDING; WHEAT;

ARTICLE; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA SEQUENCE; GENE EDITING; GENE MUTATION; NONHUMAN; PLANT CELL; TRITICUM AESTIVUM; GENETICS; METABOLISM; MUTATION; PLANT BREEDING; WHEAT;

TRITICUM AESTIVUM;

CRISPR-CAS SYSTEMS; DNA, PLANT; GENE EDITING; MUTATION; PLANT BREEDING; RIBONUCLEOPROTEINS; TRITICUM;

EID: 85010058709     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms14261     Document Type: Article

References (15)

1. Voytas, D. F. & Gao, C. Precision genome engineering and agriculture: opportunities and regulatory challenges. PLOS Biol. 12, 429-440 (2014).
2. Bortesi, L. & Fischer, R. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol. Adv. 33, 41-52 (2015).
3. Zhang, Y. et al. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nat. Commun. 7, 12617 (2016).
4. Gorbunova, V. & Levy, A. A. Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions. Nucleic Acids Res. 25, 4650-4657 (1997).
5. Kim, S., Kim, D., Cho, S. W., Kim, J. & Kim, J. S. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24, 1012-1019 (2014).
6. Zhang, F. et al. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc. Natl Acad. Sci. USA 107, 12028-12033 (2010).
7. Jones, H. D. Regulatory uncertainty over genome editing. Nat. Plants 1, 14011 (2015).
8. Woo, J. W. et al. DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins. Nat. Biotechnol. 33, 1162-1164 (2015).
9. Yang, Z. et al. SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight. Theor. Appl Genet. 125, 1057-1068 (2012).
10. Shan, Q., Wang, Y., Li, J. & Gao, C. Genome editing in rice and wheat using the CRISPR/Cas system. Nat. Protoc. 9, 2395-2410 (2014).
11. Kanchiswamy, C. N., Malnoy, M., Velasco, R., Kim, J. S. & Viola, R. Non-GMO genetically edited crop plants. Trends Biotechnol. 33, 489-491 (2015).
12. Wang, Y. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat. Biotechnol. 32, 947-951 (2014).
13. Svitashev, S., Schwartz, C., Lenderts, B., Young, J. K. & Mark Cigan, A. Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes. Nat. Commun. 7, 13274 (2016).
14. Shan, Q. et al. Targeted genome modification of crop plants using a CRISPRCas system. Nat. Biotechnol. 31, 686-688 (2013).
15. Martin-Ortigosa, S. & Wang, K. Proteolistics: A biolistic method for intracellular delivery of proteins. Transgenic Res. 23, 743-756 (2014).