High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Zhang, Kang ^a   Su, Lingqia ^a   Duan, Xuguo ^a   Liu, Lina ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Bacillus subtilis; General applicability; High level expression; Promoter optimization; Scale up production

Indexed keywords

ALKALINE PROTEINASE; BETA CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; GLUCOSE; NEUTRAL PROTEINASE; PULLULANASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; GLUCOSYLTRANSFERASE; RECOMBINANT PROTEIN;

AMYQ GENE; APRE GENE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; CONTROLLED STUDY; DUAL PROMOTER; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENETIC SCREENING; MOLECULAR DYNAMICS; NONHUMAN; NPRE GENE; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN SYNTHESIS; SCALE UP; SHAKE FLASK CULTIVATION; TIME SERIES ANALYSIS; BACILLUS AMYLOLIQUEFACIENS; BIOSYNTHESIS; FERMENTATION; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR CLONING; PLASMID; PROCEDURES;

BACILLUS AMYLOLIQUEFACIENS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CLONING, MOLECULAR; FERMENTATION; GENE EXPRESSION; GLUCOSYLTRANSFERASES; INDUSTRIAL MICROBIOLOGY; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 85013288078     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-017-0649-1     Document Type: Article

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