BioBrick™ assembly using the in-fusion PCR cloning kit

Methods in Molecular Biology

Volumn 1073, Issue , 2013, Pages 19-30

  Sleight, Sean C ^a   Sauro, Herbert M ^a  

^a University of Washington   (United States)

Author keywords

BioBrick; DNA assembly; Genetic circuits; In fusion; Plasmids; Synthetic biology

Indexed keywords

DNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA SEQUENCE; DNA SYNTHESIS; DNA TEMPLATE; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; RESTRICTION SITE; SYNTHETIC BIOLOGY;

COMPUTATIONAL BIOLOGY; DNA, RECOMBINANT; POLYMERASE CHAIN REACTION; SYNTHETIC BIOLOGY;

EID: 84884184667     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-625-2_3     Document Type: Article

References (13)

1. Knight T (2003) Idempotent vector design for standard assembly of Biobricks. DSpace http://hdl.handle.net/1721.1/21168
2. Shetty RP, Endy D, Knight TF Jr (2008) Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2:5
3. Kelly JR, Rubin AJ, Davis JH et al (2009)Measuring the activity of BioBrick promoters using an in vivo reference standard. J Biol Eng 3:4
4. Canton B, Labno A, Endy D (2008) Refinement and standardization of synthetic biological parts and devices. Nat Biotechnol 26:787-793
5. Anderson JC, Dueber JE, Leguia M et al (2010) BglBricks: a flexible standard for biological part assembly. J Biol Eng 4:1
6. Weber E, Engler C, Gruetzner R et al (2011) A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765
7. Gibson DG, Young L, Chuang R-Y et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343-345
8. Sleight SC, Bartley BA, Lieviant JA, Sauro HM (2010) In-Fusion BioBrick assembly and reengineering. Nucleic Acids Res 38:2624-2636
9. Zhu B, Cai G, Hall EO, Freeman GJ (2007) Infusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations. Biotechniques 43:354-359
10. Benoit RM, Wilhelm RN, Scherer-Becker D, Ostermeier C (2006) An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids. Protein Expr Purif 45:66-71
11. Li MZ, Elledge SJ (2007) Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods 4:251-256
12. Geu-Flores F, Nour-Eldin HH, Nielsen MT, Halkier BA (2007) USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products. Nucleic Acids Res 35:e55
13. Ellis T, Adie T, Baldwin GS (2011) DNA assembly for synthetic biology: from parts to pathways and beyond. Integr Biol 3:109-118