Cloning and overexpression of gene encoding the Pullulanase from Bacillus naganoensis in Pichia pastoris

Journal of Microbiology and Biotechnology

Volumn 16, Issue 8, 2006, Pages 1185-1191

  Xu, Bo ^a   Yang, Yun Juan ^a   Huang, Zun Xi ^a  

^a YUNNAN NORMAL UNIVERSITY   (China)

Author keywords

Bacillus naganoensis; Enzymatic properties; Expression; Pichia pastoris; Pullulanase

Indexed keywords

BACTERIAL DNA; PULLULANASE; RECOMBINANT ENZYME;

ARTICLE; BACILLUS; BACILLUS NAGANOENSIS; BACTERIAL STRAIN; CONTROLLED STUDY; ELECTROPORATION; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE INSERTION; GENE OVEREXPRESSION; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PICHIA PASTORIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

BACILLUS NAGANOENSIS; PICHIA PASTORIS;

EID: 33748692504     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Carol, A. S., J. C. Jeffrey, R. M. William, A. R. Michael, and S. Koti. 1994. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology 12: 181-184.
2. Cereghino, J. L. and J. M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 24: 45-46.
3. Clare, J., C. Scorer, R. Buckholz, and M. Romanos. 1998. Expression of EGF and HIV envelope glycoprotein. Methods Mol. Biol. 103: 209-225.
4. Clare, J. J., F. B. Rayment, S. P. Ballantine, K. Sreekrishna, and M. A. Romanos. 1991. High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integration of the gene. Biotechnology 9: 455-460.
5. Cregg, J. M., T. S. Vedvick, and W. C. Raschke. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11: 905-910.
6. Deweer Philippe, Amory Antoine. Oct. 1998. Pullulanase-producing microrganisms. U. S. patent 5,817,498.
7. Nielsen, G C., I. V. Diers, H. Outtrup, and B. E. Norman. Dec. 1985. Debranching enzyme product, preparation and use thereof. U. S. patent 4,560,651.
8. Romanos, M. A., C. A. Scorer, J. J. Clare, et al. 1992. Foreign gene expression in yeast: A review. Yeast 8: 423-488.
9. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Spring Harbor, NY, U.S.A.
10. Sreekrishna, K., L. Nelles, R. Potenz, J. Cruze, P. Mazzaferro, W. Fish, M. Fuke, et al. 1989. High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28: 4117-4125.
11. Tang, B. Y., X. H. Zhu, and J. Liu. 2001. Selection of the acid and heat-resistant pullulanase-produing strain and its fermentation conditions. Chinese Microbiol. Bull. 28: 39-43.
12. Teague, W. M., P. J. Brumm, L. N. Allen, and I. A. Brikun. Oct. 2001. Pullulanase expression constructs containing alpha-amylase promoter and leader sequence. U. S. patent 6,300,115.
13. Tomimura Eijiro. Oct. 1991. Thermoduric and aciduric pullulanase enzyme and method for its production. U. S. patent 5,055,403.
14. Wang, C., M. Eufemi, C. Turano, and A. Giartosio. 1996. Influence of the carbohydrate moiety on the stability of glycoproteins. Biochemistry 35: 7299-7307.
15. Wu, Y. P., Q. R. Jin, and X. Li. 1998. Study on production of pullulanase by microorganism. Chinese J. Biotechnol. 8: 14-17.
16. Yao, B. and C. Y. Zhang. 1998. Overexpression of the gene coding phytase in Pichia pastoris. Sci. China Ser. C Life Sci. 28: 238-243.