Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening

PLoS ONE

Volumn 12, Issue 2, 2017, Pages

  Quaglia, Daniela ^a,b   Ebert, Maximilian C C J C ^a,b   Mugford, Paul F ^c   Pelletier, Joelle N ^a,b  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b PROTEO   (Canada)

^c DSM NUTRITIONAL PRODUCTS LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACID LIPASE; FATTY ACID; ENZYME;

ARTICLE; AUTOMATION; BIOCATALYST; CANDIDA ANTARCTICA; CONTROLLED STUDY; ENZYME ENGINEERING; ENZYME SUBSTRATE; ENZYME SYNTHESIS; GENE MUTATION; GENETIC RECOMBINATION; HIGH THROUGHPUT SCREENING; HYDROLYSIS; LIPASE A GENE; MUTAGENESIS; PEPTIDE LIBRARY; PROTEIN ASSEMBLY; SYNTHETIC BIOLOGY; VISUAL DISCRIMINATION; CHEMISTRY; GENE LIBRARY; GENETICS; METABOLISM; MOLECULAR MODEL; PROCEDURES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ENZYMES; GENE LIBRARY; HIGH-THROUGHPUT SCREENING ASSAYS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STRUCTURE-ACTIVITY RELATIONSHIP; SYNTHETIC BIOLOGY;

EID: 85012159790     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0171741     Document Type: Article

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