The deubiquitinating enzyme, ubiquitin-specific peptidase 50, regulates inflammasome activation by targeting the ASC adaptor protein

FEBS Letters

Volumn 591, Issue 3, 2017, Pages 479-490

  Lee, Jae Young ^a   Seo, Dongyeob ^a   You, Jiyeon ^a   Chung, Sehee ^a   Park, Jin Seok ^a   Lee, Ji Hyung ^a   Jung, Su Myung ^a   Lee, Youn Sook ^a   Park, Seok Hee ^a  

^a Sungkyunkwan University   (South Korea)

Author keywords

ASC adaptor protein; Inflammasome; USP50 deubiquitinating enzyme

Indexed keywords

ADAPTOR PROTEIN; APOPTOSIS ASSOCIATED SPECK LIKE PROTEIN CONTAINING A CASPASE ACTIVATION AND RECRUITMENT DOMAIN PROTEIN; DEUBIQUITINASE; INFLAMMASOME; INTERLEUKIN 18; INTERLEUKIN 1BETA; INTERLEUKIN 1BETA CONVERTING ENZYME; LYSINE; NUCLEOTIDE BINDING OLIGOMERIZATION DOMAIN LIKE RECEPTOR; NUCLEOTIDE BINDING OLIGOMERIZATION DOMAIN LIKE RECEPTOR FAMILY PROTEIN 3; UBIQUITIN; UBIQUITIN SPECIFIC PEPTIDASE 50; UNCLASSIFIED DRUG; CRYOPYRIN; CYTOKINE; CYTOSKELETON PROTEIN; MUTANT PROTEIN; NLRP3 PROTEIN, HUMAN; NLRP3 PROTEIN, MOUSE; POLYUBIQUITIN; PROTEIN BINDING; PYCARD PROTEIN, HUMAN; USP50 PROTEIN, HUMAN; USP50 PROTEIN, MOUSE;

ANIMAL CELL; BONE MARROW DERIVED MACROPHAGE; CONTROLLED STUDY; CYTOKINE RELEASE; EMBRYO; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LETTER; MOUSE; NONHUMAN; OLIGOMERIZATION; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN SECRETION; PROTEIN TARGETING; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; UBIQUITINATION; ANIMAL; BIOCATALYSIS; BONE MARROW CELL; CELL LINE; CYTOLOGY; MACROPHAGE; METABOLISM; PROTEIN MULTIMERIZATION; RNA INTERFERENCE;

ANIMALS; BIOCATALYSIS; BONE MARROW CELLS; CELL LINE; CYTOKINES; CYTOSKELETAL PROTEINS; HUMANS; INFLAMMASOMES; LYSINE; MACROPHAGES; MICE; MUTANT PROTEINS; NLR FAMILY, PYRIN DOMAIN-CONTAINING 3 PROTEIN; POLYUBIQUITIN; PROTEIN BINDING; PROTEIN MULTIMERIZATION; RNA INTERFERENCE; UBIQUITIN-SPECIFIC PROTEASES; UBIQUITINATION;

EID: 85011022713     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1002/1873-3468.12558     Document Type: Article

References (28)

1. Akira S, Uematsu S and Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124, 783–801.
2. Schroder K and Tschopp J (2010) The inflammasomes. Cell 140, 821–832.
3. Broz P and Dixit VM (2016) Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol 16, 407–420.
4. Bauernfeind FG, Horvath G, Stutz A, Alnemri ES, MacDonald K, Speert D, Fernandes-Alnemri T, Wu J, Monks BG, Fitzgerald KA et al. (2009) Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression. J Immunol 183, 787–791.
5. Perregaux D and Gabel CA (1994) Interleukin-1 beta maturation and release in response to ATP and nigericin. Evidence that potassium depletion mediated by these agents is a necessary and common feature of their activity. J Biol Chem 269, 15195–15203.
6. Py BF, Kim MS, Vakifahmetoglu-Norberg H and Yuan J (2013) Deubiquitination of NLRP3 by BRCC3 critically regulates inflammasome activity. Mol Cell 49, 331–338.
7. Vande Walle L, Van Opdenbosch N, Jacques P, Fossoul A, Verheugen E, Vogel P, Beyaert R, Elewaut D, Kanneganti TD, van Loo G et al. (2014) Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis. Nature 512, 69–73.
8. Duong BH, Onizawa M, Oses-Prieto JA, Advincula R, Burlingame A, Malynn BA and Ma A (2015) A20 restricts ubiquitination of pro-interleukin-1beta protein complexes and suppresses NLRP3 inflammasome activity. Immunity 42, 55–67.
9. Han S, Lear TB, Jerome JA, Rajbhandari S, Snavely CA, Gulick DL, Gibson KF, Zou C, Chen BB and Mallampalli RK (2015) Lipopolysaccharide primes the NALP3 inflammasome by inhibiting its Ubiquitination and degradation mediated by the SCFFBXL2 E3 ligase. J Biol Chem 290, 18124–18133.
10. Lu A, Magupalli VG, Ruan J, Yin Q, Atianand MK, Vos MR, Schroder GF, Fitzgerald KA, Wu H and Egelman EH (2014) Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes. Cell 156, 1193–1206.
11. Cai X, Chen J, Xu H, Liu S, Jiang QX, Halfmann R and Chen ZY (2014) Prion-like polymerization underlies signal transduction in antiviral immune defense and inflammasome activation. Cell 156, 1207–1222.
12. Rodgers MA, Bowman JW, Fujita H, Orazio N, Shi M, Liang Q, Amatya R, Kelly TJ, Iwai K, Ting J et al. (2014) The linear ubiquitin assembly complex (LUBAC) is essential for NLRP3 inflammasome activation. J Exp Med 211, 1333–1347.
13. Shi CS, Shenderov K, Huang NN, Kabat J, Abu-Asab M, Fitzgerald KA, Sher A and Kehrl JH (2012) Activation of autophagy by inflammatory signals limits IL-1beta production by targeting ubiquitinated inflammasomes for destruction. Nat Immunol 13, 255–263.
14. Jung SM, Lee JH, Park J, Oh YS, Lee SK, Park JS, Lee YS, Kim JH, Lee JY, Bae YS et al. (2013) Smad6 inhibits non-canonical TGF-beta1 signalling by recruiting the deubiquitinase A20 to TRAF6. Nat Commun 4, 2562.
15. Aressy B, Jullien D, Cazales M, Marcellin M, Bugler B, Burlet-Schiltz O and Ducommun B (2010) A screen for deubiquitinating enzymes involved in the G(2)/M checkpoint identifies USP50 as a regulator of HSP90-dependent Wee1 stability. Cell Cycle 9, 3815–3822.
16. Swantek JL, Christerson L and Cobb MH (1999) Lipopolysaccharide-induced tumor necrosis factor-alpha promoter activity is inhibitor of nuclear factor-kappaB kinase-dependent. J Biol Chem 274, 11667–11671.
17. Stutz A, Horvath GL, Monks BG and Latz E (2013) ASC speck formation as a readout for inflammasome activation. Methods Mol Biol 1040, 91–101.
18. Broderick L and Hoffman HM (2014) cASCading specks. Nat Immunol 15, 698–700.
19. Hara H, Tsuchiya K, Kawamura I, Fang R, Hernandez-Cuellar E, Shen Y, Mizuguchi J, Schweighoffer E, Tybulewicz V and Mitsuyama M (2013) Phosphorylation of the adaptor ASC acts as a molecular switch that controls the formation of speck-like aggregates and inflammasome activity. Nat Immunol 14, 1247–1255.
20. Ye Y, Scheel H, Hofmann K and Komander D (2009) Dissection of USP catalytic domains reveals five common insertion points. Mol BioSyst 5, 1797–1808.
21. Zhang H, Wang D, Zhong H, Luo R, Shang M, Liu D, Chen H, Fang L and Xiao S (2015) Ubiquitin-specific protease 15 negatively regulates virus-induced type I interferon signaling via catalytically-dependent and -independent mechanisms. Sci Rep 5, 11220.
22. Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, Latz E and Fitzgerald KA (2009) AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC. Nature 458, 514–518.
23. Bauernfeind F, Bartok E, Rieger A, Franchi L, Núñez G and Hornung V (2011) Cutting edge: reactive oxygen species inhibitors block priming, but not activation of the NLRP3 inflammasome. J Immunol 187, 613–617.
24. Strowig T, Henao-Mejia J, Elinav E and Flavell R (2012) Inflammasomes in health and disease. Nature 481, 278–286.
25. Hoffman HM and Brydges SD (2011) Genetic and molecular basis of inflammasome-mediated disease. J Biol Chem 286, 10889–10896.
26. Liu J, Qian C and Cao X (2016) Post-translational modification control of innate immunity. Immunity 45, 15–30.
27. Jiang X and Chen ZY (2012) The role of ubiquitylation in immune defence and pathogen evasion. Nat Rev Immunol 12, 35–48.
28. Guan K, Wei C, Zheng Z, Song T, Wu F, Zhang Y, Cao Y, Ma S, Chen W, Xu Q et al. (2015) MAVS promotes inflammasome activation by targeting ASC for K63-linked Ubiquitination via the E3 ligase TRAF3. J Immunol 194, 4880–4890.