ASC speck formation as a readout for inflammasome activation

Methods in Molecular Biology

Volumn 1040, Issue , 2013, Pages 91-101

  Stutz, Andrea ^a   Horvath, Gabor L ^a   Monks, Brian G ^a,b   Latz, Eicke ^a,b,c  

^a UNIVERSITY HOSPITAL BONN   (Germany)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^c GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

Author keywords

AIM2; ASC; Inflammasome; Microscopy; NLRC4; NLRP3; NLRP6; Protein speck; Quantitative image analysis

Indexed keywords

ADAPTOR PROTEIN; ASC PROTEIN; INFLAMMASOME; INTERLEUKIN 1BETA CONVERTING ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; FLUORESCENCE ANALYSIS; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; MACROPHAGE; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

EID: 84880499903     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-523-1_8     Document Type: Article

References (2)

1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J (1999) ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem 274(48): 33835-33838
2. Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9(7):671-675