Crystal structure of Sec10, a subunit of the exocyst complex

Scientific Reports

Volumn 7, Issue , 2017, Pages

  Chen, Jianxing ^a,b   Yamagata, Atsushi ^a,b   Kubota, Keiko ^a   Sato, Yusuke ^a,b   Goto Ito, Sakurako ^a,b   Fukai, Shuya ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

SEC10 PROTEIN, ZEBRAFISH; VESICULAR TRANSPORT PROTEIN; ZEBRAFISH PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; METABOLISM; PROTEIN SUBUNIT; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; STATIC ELECTRICITY; X RAY CRYSTALLOGRAPHY; ZEBRA FISH;

AMINO ACID SEQUENCE; ANIMALS; CRYSTALLOGRAPHY, X-RAY; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; SEQUENCE ALIGNMENT; STATIC ELECTRICITY; VESICULAR TRANSPORT PROTEINS; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 85010058347     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep40909     Document Type: Article

References (56)

1. Wu, L. G., Hamid, E., Shin, W. & Chiang, H. C. Exocytosis and endocytosis: modes, functions, and coupling mechanisms. Annu Rev Physiol 76, 301-331 (2014).
2. Wu, B. & Guo, W. The exocyst at a glance. J Cell Sci 128, 2957-2964 (2015).
3. Novick, P. , Field, C. & Schekman, R. Identifcation of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21, 205-215 (1980).
4. TerBush, D. R., Maurice, T., Roth, D. & Novick, P. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15, 6483-6494 (1996).
5. Kee, Y. et al. Subunit structure of the mammalian exocyst complex. Proc Natl Acad Sci USA 94, 14438-14443 (1997).
6. Zajac, A., Sun, X., Zhang, J. & Guo, W. Cyclical regulation of the exocyst and cell polarity determinants for polarized cell growth. Mol Biol Cell 16, 1500-1512 (2005).
7. Liu, J. & Guo, W. The exocyst complex in exocytosis and cell migration. Protoplasma 249, 587-597 (2012).
8. Das, A. & Guo, W. Rabs and the exocyst in ciliogenesis, tubulogenesis and beyond. Trends Cell Biol 21, 383-386 (2011).
9. Vega, I. E. & Hsu, S. C. The exocyst complex associates with microtubules to mediate vesicle targeting and neurite outgrowth. J Neurosci 21, 3839-3848 (2001).
10. Bodemann, B. O. et al. RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly. Cell 144, 253-267 (2011).
11. Barlowe & C. Coupled ER to Golgi transport reconstituted with purifed cytosolic proteins. J Cell Biol 139, 1097-1108 (1997).
12. Yu, I. M. & Hughson, F. M. Tethering factors as organizers of intracellular vesicular trafc. Annu Rev Cell Dev Biol 26, 137-156 (2010).
13. Yamashita, M. et al. Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3. Nat Struct Mol Biol 17, 180-186 (2010).
14. Baek, K. et al. Structure-function study of the N-terminal domain of exocyst subunit Sec3. J Biol Chem 285, 10424-10433 (2010).
15. Fukai, S., Matern, H. T., Jagath, J. R., Scheller, R. H. & Brunger, A. T. Structural basis of the interaction between RalA and Sec5, a subunit of the Sec6/8 complex. EMBO J 22, 3267-3278 (2003).
16. Sivaram, M. V., Furgason, M. L., Brewer, D. N. & Munson, M. The structure of the exocyst subunit Sec6p defnes a conserved architecture with diverse roles. Nat Struct Mol Biol 13, 555-556 (2006).
17. Wu, S., Mehta, S. Q., Pichaud, F. , Bellen, H. J. & Quiocho, F. A. Sec15 interacts with Rab11 via a novel domain and afects Rab11 localization in vivo. Nat Struct Mol Biol 12, 879-885 (2005).
18. Moore, B. A., Robinson, H. H. & Xu, Z. The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst. J Mol Biol 371, 410-421 (2007).
19. Dong, G., Hutagalung, A. H., Fu, C., Novick, P. & Reinisch, K. M. The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nat Struct Mol Biol 12, 1094-1100 (2005).
20. Jin, R. et al. Exo84 and Sec5 are competitive regulatory Sec6/8 efectors to the RalA GTPase. EMBO J 24, 2064-2074 (2005).
21. Zhang, C. et al. Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis. Proc Natl Acad Sci USA 113, E41-50 (2016).
22. Croteau, N. J., Furgason, M. L., Devos, D. & Munson, M. Conservation of helical bundle structure between the exocyst subunits. PLoS One 4, e4443 (2009).
23. Heider, M. R. et al. Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex. Nat Struct Mol Biol (2015).
24. Katoh, Y., Nozaki, S., Hartanto, D., Miyano, R. & Nakayama, K. Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fuorescent fusion proteins. J Cell Sci 128, 2351-2362 (2015).
25. Lipschutz, J. H. et al. Exocyst is involved in cystogenesis and tubulogenesis and acts by modulating synthesis and delivery of basolateral plasma membrane and secretory proteins. Mol Biol Cell 11, 4259-4275 (2000).
26. Roth, D., Guo, W. & Novick, P. Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell 9, 1725-1739 (1998).
27. Prigent, M. et al. ARF6 controls post-endocytic recycling through its downstream exocyst complex effector. J Cell Biol 163, 1111-1121 (2003).
28. D'Souza-Schorey, C., Li, G., Colombo, M. I. & Stahl, P. D. A regulatory role for ARF6 in receptor-mediated endocytosis. Science 267, 1175-1178 (1995).
29. Fielding, A. B. et al. Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane trafc in cytokinesis. EMBO J 24, 3389-3399 (2005).
30. Zuo, X., Guo, W. & Lipschutz, J. H. The exocyst protein Sec10 is necessary for primary ciliogenesis and cystogenesis in vitro. Mol Biol Cell 20, 2522-2529 (2009).
31. Ward, J. J., Sodhi, J. S., McGufn, L. J., Buxton, B. F. & Jones, D. T. Prediction and functional analysis of native disorder in proteins from the three kingdoms of life. J Mol Biol 337, 635-645 (2004).
32. Richardson, B. C. et al. Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene. Proc Natl Acad Sci USA 106, 13329-13334 (2009).
33. Tripathi, A., Ren, Y., Jefrey, P. D. & Hughson, F. M. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16, 114-123 (2009).
34. Perez-Victoria, F. J. et al. Structural basis for the wobbler mouse neurodegenerative disorder caused by mutation in the Vps54 subunit of the GARP complex. Proc Natl Acad Sci USA 107, 12860-12865 (2010).
35. Holm, L. & Rosenstrom, P. Dali server: conservation mapping in 3D. Nucleic Acids Res 38, W545-549 (2010).
36. Kimura, S. et al. Distinct Roles for the N- and C-terminal regions of M-Sec in plasma membrane deformation during tunneling nanotube formation. Sci Rep 6, 33548 (2016).
37. Velvarska, H. & Niessing, D. Structural insights into the globular tails of the human type V myosins Myo5a, Myo5b, And Myo5c. PLoS One 8, e82065 (2013).
38. Li, W. et al. The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors. Structure 19, 1443-1455 (2011).
39. Munson, M., Chen, X., Cocina, A. E., Schultz, S. M. & Hughson, F. M. Interactions within the yeast t-SNARE Sso1p that control SNARE complex assembly. Nat Struct Biol 7, 894-902 (2000).
40. He, B., Xi, F., Zhang, X., Zhang, J. & Guo, W. Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J 26, 4053-4065 (2007).
41. Songer, J. A. & Munson, M. Sec6p anchors the assembled exocyst complex at sites of secretion. Mol Biol Cell 20, 973-982 (2009).
42. Papaleo, E. et al. The role of protein loops and linkers in conformational dynamics and allostery. Chem. Rev. 116, 6391-6423 (2016).
43. Fridmann-Sirkis, Y. , Kent, H. M., Lewis, M. J., Evans, P. R. & Pelham, H. R. Structural analysis of the interaction between the SNARE Tlg1 and Vps51. Trafc 7, 182-190 (2006).
44. Jin, Y. et al. Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex. Dev Cell 21, 1156-1170 (2011).
45. Ha, J. Y. et al. Molecular architecture of the complete COG tethering complex. Nat Struct Mol Biol 23, 758-760 (2016).
46. Chou, H. T., Dukovski, D., Chambers, M. G., Reinisch, K. M. & Walz, T. CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol 23, 761-763 (2016).
47. Otwinowski, Z. & Minor, W. Processing of X-ray difraction data collected in oscillation mode. Methods Enzymol part A 276, 307-326 (1997).
48. Winn, M. D. et al. Overview of the CCP4 suite and current developments. Acta Crystallogr D 67, 235-242 (2011).
49. Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D 66, 213-221 (2010).
50. Cowtan, K. The Buccaneer sofware for automated model building. 1. Tracing protein chains. Acta Crystallogr D 62, 1002-1011 (2006).
51. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D 66, 486-501 (2010).
52. Baker, N. A., Sept, D., Joseph, S., Holst, M. J. & McCammon, J. A. Electrostatics of nanosystems: application to microtubules and the ribosome. Proc Natl Acad Sci USA 98, 10037-10041 (2001).
53. Eisenberg, D., Schwarz, E., Komaromy, M. & Wall, R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 179, 125-142 (1984).
54. Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947-2948 (2007).
55. Ashkenazy, H. et al. ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules. Nucleic Acids Res 44, W344-W350 (2016).
56. Robert, X. & Gouet, P. Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Res 42, W320-324 (2014).