Architectures of multisubunit complexes revealed by a visible immunoprecipitation assay using fluorescent fusion proteins

Journal of Cell Science

Volumn 128, Issue 12, 2015, Pages 2351-2362

  Katoh, Yohei ^a   Nozaki, Shohei ^a   Hartanto, David ^a   Miyano, Rie ^a   Nakayama, Kazuhisa ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Anti GFP nanobody; BBSome; Exocyst; Fluorescent fusion protein; Membrane traffic; Protein protein interaction

Indexed keywords

EXOCYST; GREEN FLUORESCENT PROTEIN; NANOBODY; RED FLUORESCENT PROTEIN; BBIP1 PROTEIN, HUMAN; BBS4 PROTEIN, HUMAN; CARRIER PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; MULTIPROTEIN COMPLEX; PHOTOPROTEIN; PROTEIN; PROTEIN BINDING; PROTEIN SUBUNIT; TTC8 PROTEIN, HUMAN; VESICULAR TRANSPORT PROTEIN;

ARTICLE; BBSOME SUBUNIT; CELL FUNCTION; CELL LYSATE; CENTROSOME; CLINICAL EVALUATION; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; ISOTHERMAL TITRATION CALORIMETRY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN SUBUNIT; TETRATRICOPEPTIDE REPEAT; VISIBLE IMMUNOPRECIPITATION; CELL CULTURE; CELL MEMBRANE; ENZYME IMMUNOASSAY; HEK293 CELL LINE; METABOLISM; PROTEIN TRANSPORT; TWO HYBRID SYSTEM; WESTERN BLOTTING;

BLOTTING, WESTERN; CARRIER PROTEINS; CELL MEMBRANE; CELLS, CULTURED; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOPRECIPITATION; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MULTIPROTEIN COMPLEXES; PROTEIN BINDING; PROTEIN INTERACTION MAPS; PROTEIN SUBUNITS; PROTEIN TRANSPORT; PROTEINS; SINGLE-DOMAIN ANTIBODIES; TWO-HYBRID SYSTEM TECHNIQUES; VESICULAR TRANSPORT PROTEINS;

EID: 84932108013     PISSN: 00219533     EISSN: 14779137     Source Type: Journal    
DOI: 10.1242/jcs.168740     Document Type: Article

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