Conservation of helical bundle structure between the exocyst subunits

PLoS ONE

Volumn 4, Issue 2, 2009, Pages

  Croteau, Nicole J ^a   Furgason, Melonnie L M ^a   Devos, Damien ^b   Munson, Mary ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EXOCYST; FUNGAL PROTEIN; PROTEIN SEC10P; PROTEIN SUBUNIT; SNARE PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION;

EUKARYOTA;

EID: 84887212464     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004443     Document Type: Article

References (45)

1. TerBush DR, Maurice T, Roth D, Novick P (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. Embo J 15: 6483-6494.
2. Guo W, Roth D, Walch-Solimena C, Novick P (1999) The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. Embo J 18: 1071-1080.
3. Munson M, Novick P (2006) The exocyst defrocked, a framework of rods revealed. Nat Struct Mol Biol 13: 577-581.
4. Wu H, Rossi G, Brennwald P (2008) The ghost in the machine: small GTPases as spatial regulators of exocytosis. Trends Cell Biol 18: 397-404.
5. Wiederkehr A, De Craene JO, Ferro-Novick S, Novick P (2004) Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol 167: 875-887.
6. Sivaram MV, Saporita JA, Furgason ML, Boettcher AJ, Munson M (2005) Dimerization of the exocyst protein Sec6p and its interaction with the t-SNARE Sec9p. Biochemistry 44: 6302-6311.
7. Whyte JR, Munro S (2002) Vesicle tethering complexes in membrane traffic. J Cell Sci 115: 2627-2637.
8. Lupashin V, Sztul E (2005) Golgi tethering factors. Biochim Biophys Acta 1744: 325-339.
9. Cavanaugh LF, Chen X, Richardson BC, Ungar D, Pelczer I, et al. (2007) Structural analysis of conserved oligomeric Golgi complex subunit 2. J Biol Chem 282: 23418-23426.
10. Kummel D, Heinemann U (2008) Diversity in structure and function of tethering complexes: evidence for different mechanisms in vesicular transport regulation. Curr Protein Pept Sci 9: 197-209.
11. Koumandou VL, Dacks JB, Coulson RM, Field MC (2007) Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins. BMC Evol Biol 7: 29.
12. Dong G, Hutagalung AH, Fu C, Novick P, Reinisch KM (2005) The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif. Nat Struct Mol Biol 12: 1094-1100.
13. Hamburger ZA, Hamburger AE, West AP, Weis WI (2006) Crystal Structure of the S. cerevisiae Exocyst Component Exo70p. J Mol Biol 356: 9-21.
14. Moore BA, Robinson HH, Xu Z (2007) The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst. J Mol Biol 371: 410-421.
15. Sivaram MV, Furgason ML, Brewer DN, Munson M (2006) The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles. Nat Struct Mol Biol 13: 555-556.
16. Wu S, Mehta SQ, Pichaud F, Bellen HJ, Quiocho FA (2005) Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo. Nat Struct Mol Biol 12: 879-885.
17. Derewenda ZS (2004) The use of recombinant methods and molecular engineering in protein crystallization. Methods 34: 354-363.
18. Dokudovskaya S, Williams R, Devos D, Sali A, Chait BT, et al. (2006) Protease accessibility laddering: a proteomic tool for probing protein structure. Structure 14: 653-660.
19. Esposito D, Chatterjee DK (2006) Enhancement of soluble protein expression through the use of fusion tags. Curr Opin Biotechnol 17: 353-358.
20. Peti W, Page R (2007) Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Protein Expr Purif 51: 1-10.
21. Graslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, et al. (2008) Protein production and purification. Nat Methods 5: 135-146.
22. Yoon J, Kang Y, Kim K, Park J, Kim Y (2005) Identification and purification of a soluble region of BubR1: a critical component of the mitotic checkpoint complex. Protein Expr Purif 44: 1-9.
23. Chen Y, Qiu S, Luan CH, Luo M (2007) Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins. BMC Biotechnol 7: 45.
24. Graslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, et al. (2008) The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr Purif 58: 210-221.
25. Soding J, Biegert A, Lupas AN (2005) The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res 33: W244-248.
26. Soding J (2005) Protein homology detection by HMM-HMM comparison. Bioinformatics 21: 951-960.
27. Roth D, Guo W, Novick P (1998) Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell 9: 1725-1739.
28. Guo W, Grant A, Novick P (1999) Exo84p is an exocyst protein essential for secretion. J Biol Chem 274: 23558-23564.
29. Hsu SC, Hazuka CD, Roth R, Foletti DL, Heuser J, et al. (1998) Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments. Neuron 20: 1111-1122.
30. Whyte JR, Munro S (2001) The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic. Dev Cell 1: 527-537.
31. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, et al. (2000) The Protein Data Bank. Nucleic Acids Res 28: 235-242.
32. Pashkova N, Jin Y, Ramaswamy S, Weisman LS (2006) Structural basis for myosin V discrimination between distinct cargoes. Embo J 25: 693-700.
33. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
34. Mott HR, Nietlispach D, Hopkins LJ, Mirey G, Camonis JH, et al. (2003) Structure of the GTPase-binding domain of Sec5 and elucidation of its Ral binding site. J Biol Chem 278: 17053-17059.
35. Fukai S, Matern HT, Jagath JR, Scheller RH, Brunger AT (2003) Structural basis of the interaction between RalA and Sec5, a subunit of the sec6/8 complex. Embo J 22: 3267-3278.
36. Jin R, Junutula JR, Matern HT, Ervin KE, Scheller RH, et al. (2005) Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase. Embo J 24: 2064-2074.
37. Nallamsetty S, Waugh DS (2006) Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners. Protein Expr Purif 45: 175-182.
38. Thomas JG, Ayling A, Baneyx F (1997) Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. To fold or to refold. Appl Biochem Biotechnol 66: 197-238.
39. Scholtz JM, Barrick D, York EJ, Stewart JM, Baldwin RL (1995) Urea unfolding of peptide helices as a model for interpreting protein unfolding. Proc Natl Acad Sci U S A 92: 185-189.
40. Devos D, Dokudovskaya S, Williams R, Alber F, Eswar N, et al. (2006) Simple fold composition and modular architecture of the nuclear pore complex. Proc Natl Acad Sci U S A 103: 2172-2177.
41. Jones DT (1999) Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol 292: 195-202.
42. Sali A, Blundell TL (1993) Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 234: 779-815.
43. Shen MY, Sali A (2006) Statistical potential for assessment and prediction of protein structures. Protein Sci 15: 2507-2524.
44. Melo F, Sanchez R, Sali A (2002) Statistical potentials for fold assessment. Protein Sci 11: 430-448.
45. Rosen H (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys 67: 10-15.