The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts

Journal of Traditional Medicines

Volumn 21, Issue 2, 2004, Pages 87-93

  Satoh, Akiko ^a   Yokozawa, Takako ^a   Tanaka, Takashi ^b   Okamoto, Takuya ^c   Sei, Yasuo ^c  

^a UNIVERSITY OF TOYAMA   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

^c ISKRA Industry Co Ltd   (Japan)

Author keywords

aging; free radical; hydrogen peroxide; Kangen karyu; WI 38

Indexed keywords

EID: 85009579931     PISSN: 18801447     EISSN: 18813747     Source Type: Journal    
DOI: 10.11339/jtm.21.87     Document Type: Article

References (37)

1. Hayflick, L. and Moorhead, P.S.: The several cultivation of human diploid cell strains. Exp. Cell Res. 25, 585-621, 1961.
2. Hayflick, L.: The cell biology of human aging. N. Engl. J. Med. 295, 1302-1308, 1976.
3. Harley, C.B.: Telomere loss: mitotic clock or genetic time bomb? Mutat. Res. 256, 271-282, 1991.
4. Dimri, G.P., Lee, X., Basile, G., Acosta, M., Scott, G., Roskelley, C., Medrano, E.E., Linskens, M., Rubelj, I., Pereira-Smith, O., Peacocke, M. and Campisi, J.: A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92, 9363-9367, 1995.
5. Linskens, M.H., Harley, C.B., West, M.D., Campisi, J. and Hayflick, L.: Replicative senescence and cell death. Science 267, 17, 1995.
6. Campisi, J., Dimri, G.P., Nehlin, J.O., Testori, A. and Yoshimoto, K.: Coming of age in culture. Exp. Gerontol. 31, 7-12, 1996.
7. Campisi, J.: Cancer, aging and cellular senescence. In Vivo 14, 183-188, 2000.
8. Toussaint, O., Medrano, E.E. and Zglinicki, T.: Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes. Exp. Gerontol. 35, 927-945, 2000.
9. Allen, R.G., Tresini, M., Keogh, B.P., Doggett, D.L. and Cristofalo, V.J.: Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38). J. Cell Physiol. 180, 114-122, 1999.
10. Dumont, P., Burton, M., Chen, Q.M., Gonos, E.S., Frippiat, C., Mazarati, J.B., Eliaers, F., Remacle, J. and Toussaint, O.: Induction of replicative senescence biomarkers by sublethal oxidative stresses in normal human fibroblast. Free Radic. Biol. Med. 28, 361-373, 2000.
11. Wolf, F.I., Torsello, A., Covacci, V., Fasanella, S., Montanari, M., Boninsegna, A. and Cittadini, A.: Oxidative DNA damage as a marker of aging in WI-38 human fibroblasts. Exp. Gerontol. 37, 647-656, 2002.
12. Takahashi, H.: Clinical trial of prescription of Kaketsukao. Clin. J. Chinese Med. 12, 145-151, 1991.
13. Gao, M., Ikeda, K., Noguchi, T., Mori, K. and Yamori, Y.: Studies on preventive effect of ‘Kangen-karyu’, Chinese herbal medicine, on stroke in SHR-SP. J. Trad. Med. 18, 245-250, 2001.
14. Makino, T., Wakushima, H., Okamoto, T., Okukubo, Y., Deguchi, Y. and Kano, Y.: Pharmacokinetic interactions between warfarin and kangen-karyu, a Chinese traditional herbal medicine, and their synergistic action. J. Ethnopharmacol. 82, 35-40, 2002.
15. Makino, T., Wakushima, H., Okamoto, T., Okukubo, Y., Saito, K. and Kano, Y.: Effects of Kangen-karyu on coagulation system and platelet aggregation in mice. Biol. Pharm. Bull. 25, 523-525, 2002.
16. Takahashi, M., Sugaya, K. and Kubota, K.: Kangenkaryu prevents the decrease of cholinergic markers following the nucleus basalis magnocellularis lesion. Jpn. J. Pharmacol. 60, 307-310, 1992.
17. Satoh, A., Yokozawa, T., Cho, E.J., Okamoto, T. and Sei, Y.: Antioxidative effects related to the potential anti-aging properties of the Chinese prescription Kangen-karyu and Carthami Flos. Arch. Gerontol. Geriatr. (accepted).
18. Cristofalo, V.J. and Charpentier, R.: A standard procedure for cultivating human diploid fibroblastlike cells to study cellular aging. J. Tissue Culture Methods 6, 117-121, 1980.
19. Wang, H. and Joseph, J.A.: Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic. Biol. Med. 27, 612-616, 1999.
20. Mihara, M. and Uchiyama, M.: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86, 271-278, 1978.
21. Osseni, R.A., Rat, P., Bogdan, A., Warnet, J.M. and Touitou, Y.: Evidence of prooxidant and antioxidant action of melatonin on human liver cell line HepG2. Life Sci. 68, 387-399, 2000.
22. Block, A.L., Bauer, K.D., Williams, T.J. and Seidenfeld, J.: Experimental parameters and a biological standard for acridine orange detection of drug-induced alterations in chromatin condensation. Cytometry 8, 163-169, 1987.
23. Ho, H.Y., Cheng, M.L., Lu, F.J., Chou, Y.H., Stern, A., Liang, C.M. and Chiu, D.T.: Enhanced oxidative stress and accelerated cellular senescence in glucose-6-phosphate dehydrogenase (G6PD)-deficient human fibroblasts. Free Radic. Biol. Med. 29, 156-169, 2000.
24. Mosmann, T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63, 1983.
25. Toussaint, O., Michiels, C., Raes, M. and Remacle, J.: Cellular aging and the importance of energetic factors. Exp. Gerontol. 30, 1-22, 1995.
26. Chen, Q.M., Bartholomew, J.C., Campisi, J., Acosta, M., Reagan, J.D. and Ames, B.N.: Molecular analysis of H2O2-induced senescent-like growth arrest in normal human fibroblasts: p53 and Rb control G1 arrest but not cell replication. Biochem. J. 332, 43-50, 1998.
27. Okada, Y. and Okada, M.: Effect of a radical scavenger “water soluble protein” from broad beans (Vicia faba) on antioxidative enzyme activity in cellular aging. J. Nutr. Sci. Vitaminol. 46, 1-6, 2000.
28. Lee, H.C., Yin, P.H., Lu, C.Y., Chi, C.W. and Wei, Y.H.: Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells. Biochem. J. 348, 425-432, 2000.
29. Sohal, R.S., Ku, H.H., Agarwal, S., Forster, M.J. and Lal, H.: Oxidative damage, mitochondrial oxidant generation and antioxidant refenses during aging and in response to food restriction in the mouse. Mech. Ageing Dev. 74, 121-133, 1994.
30. Teramoto, S., Tomita, T., Matsui, H., Ohga, E., Matsuse, T. and Ouchi, Y.: Hydrogen peroxide-induced apoptosis and necrosis in human lung fibroblasts: protective roles of glutathione. Jpn. J. Pharmacol. 79, 33-40, 1999.
31. Morliere, P. and Santus, R.: Pro-oxidant role of superoxide dismutase in ultraviolet-A-induced lipid peroxidation in cultured normal human skin fibroblasts. Eur. J. Biochem. 256, 184-189, 1998.
32. Naderi, J., Hung, M. and Pandey, S.: Oxidative stress-induced apoptosis in dividing fibroblasts involves activation of p38 MAP kinase and over-expression of Bax: resistance of quiescent cells to oxidative stress. Apoptosis 8, 91-100, 2003.
33. Hayflick, L.: Current theories of biological aging. Fed. Proc. 34, 9-13, 1975.
34. Allsopp, R.C., Vaziri, H., Patterson, C., Goldstein, S., Younglai, E.V., Futcher, A.B., Greider, C.W. and Harley, C.B.: Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA 89, 10114-10118, 1992.
35. Oshima, J., Campisi, J., Tannock, T.C. and Martin, G.M.: Regulation of c-fos expression in senescing Werner syndrome fibroblasts differs from that observed in senescing fibroblasts from normal donors. J. Cell Physiol. 162, 277-283, 1995.
36. Adelfalk, C., Lorenz, M., Serra, V., von Zglinicki, T., Hirsch-Kauffmann, M. and Schweiger, M.: Accelerated telomere shortening in Fanconi anemia fibroblasts-a longitudinal study. FEBS Lett. 506, 22-26, 2001.
37. Yegorov, Y.E. and Zelenin, A.V.: Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts. FEBS Lett. 541, 6-10, 2003.