Identification and fine mapping of two blast resistance genes in rice cultivar 93-11

Crop Journal

Volumn 1, Issue 1, 2013, Pages 2-14

  Lei, Cailin ^a   Hao, Kun ^a   Yang, Yilong ^a   Ma, Jian ^a   Wang, Shuai ^a   Wang, Jiulin ^a   Cheng, Zhijun ^a   Zhao, Shasha ^a   Zhang, Xin ^a   Guo, Xiuping ^a   Wang, Chunming ^b   Wan, Jianmin ^a,b  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Gene mapping; Magnaporthe oryzae; Oryza sativa; Resistance gene; Rice blast

Indexed keywords

EID: 85007265549     PISSN: 20955421     EISSN: 22145141     Source Type: Journal    
DOI: 10.1016/j.cj.2013.07.007     Document Type: Article

References (79)

1. [1] Siluê, D., Notteghem, J.L., Tharreau, D., Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem. Phytopathology 82 (1992), 577–580.
2. [2] Zeigler, R.S., Tohme, J., Nelson, R., Levy, M., Correa Victoria, F.J., Lineage exclusion: a proposal for linking blast population analysis to resistance breeding. Zeigler, R.S., Leong, S.A., Teng, P.S., (eds.) Rice Blast Disease, 1994, CAB international, Wallingford, UK, 267–292.
3. [3] Jia, Y., Bryan, G.T., Farrall, L., Valent, B., Natural variation at the Pi-ta rice blast resistance locus. Phytopathology 93 (2003), 1452–1459.
4. [4] Mackill, D.J., Bonman, J.M., Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82 (1992), 746–749.
5. [5] Zhou, E., Jia, Y., Correll, J., Lee, F.N., Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence. Fung Genet. Biol. 44 (2007), 1024–1034.
6. [6] Dai, Y., Jia, Y., Correll, J., Wang, X., Wang, Y., Diversification and evolution of the avirulence gene AVR-Pita1 in field isolates of Magnaporthe oryzae. Fung Genet. Biol. 47 (2010), 973–980.
7. [7] Hittalmani, S., Parco, A., Mew, T.V., Zeigler, R.S., Huang, N., Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor. Appl. Genet. 100 (2000), 1121–1128.
8. [8] Goff, S.A., Ricke, D., Lan, T.H., Presting, G., Wang, R., Dunn, M., Glazebrook, J., Sessions, A., Oeller, P., Varma, H., Hadley, D., Hutchison, D., Martin, C., Katagiri, F., Lange, B.M., Moughamer, T., Xia, Y., Budworth, P., Zhong, J., Miguel, T., Paszkowski, U., Zhang, S., Colbert, M., Sun, W.L., Chen, L., Cooper, B., Park, S., Wood, T.C., Mao, L., Quail, P., Wing, R., Dean, R., Yu, Y., Zharkikh, A., Shen, R., Sahasrabudhe, S., Thomas, A., Cannings, R., Gutin, A., Pruss, D., Reid, J., Tavtigian, S., Mitchell, J., Eldredge, G., Scholl, T., Miller, R.M., Bhatnagar, S., Adey, N., Rubano, T., Tusneem, N., Robinson, R., Feldhaus, J., Macalma, T., Oliphant, A., Briggs, S., A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296 (2002), 92–100.
9. [9] Yu, J., Hu, S., Wang, J., Wong, G.K., Li, S., Liu, B., Deng, Y., Dai, L., Zhou, Y., Zhang, X., Cao, M., Liu, J., Sun, J., Tang, J., Chen, Y., Huang, X., Lin, W., Ye, C., Tong, W., Cong, L., Geng, J., Han, Y., Li, L., Li, W., Hu, G., Huang, X., Li, W., Li, J., Liu, Z., Li, L., Liu, J., Qi, Q., Liu, J., Li, L., Li, T., Wang, X., Lu, H., Wu, T., Zhu, M., Ni, P., Han, H., Dong, W., Ren, X., Feng, X., Cui, P., Li, X., Wang, H., Xu, X., Zhai, W., Xu, Z., Zhang, J., He, S., Zhang, J., Xu, J., Zhang, K., Zheng, X., Dong, J., Zeng, W., Tao, L., Ye, J., Tan, J., Ren, X., Chen, X., He, J., Liu, D., Tian, W., Tian, C., Xia, H., Bao, Q., Li, G., Gao, H., Cao, T., Wang, J., Zhao, W., Li, P., Chen, W., Wang, X., Zhang, Y., Hu, J., Wang, J., Liu, S., Yang, J., Zhang, G., Xiong, Y., Li, Z., Mao, L., Zhou, C., Zhu, Z., Chen, R., Hao, B., Zheng, W., Chen, S., Guo, W., Li, G., Liu, S., Tao, M., Wang, J., Zhu, L., Yuan, L., Yang, H., A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296 (2002), 79–92.
10. [10] IRGSP, The map-based sequence of the rice genome. Nature 436 (2005), 793–800.
11. [11] Ballini, E., Morel, J.B., Droc, G., Price, A., Courtois, B., Notteghem, J.L., Tharreau, D., A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance. Mol. Plant Microbe Interact. 21 (2008), 859–868.
12. [12] Koide, Y., Kobayashi, N., Xu, D., Fukuta, Y., Blast resistance genes and their selection markers in rice (Oryza sativa L.). JIRCA Work Rep. 63 (2009), 95–122.
13. [13] Liu, J., Wang, X., Mitchell, T., Hu, Y., Liu, X., Dai, L., Wang, G., Recent progress and understanding of the molecular mechanisms of the rice-Magnaporthe oryzae interaction. Mol. Plant Pathol. 11 (2010), 419–427.
14. [14] Wisser, R.J., Sun, Q., Hulbert, S.H., Kresovich, S., Nelson, R.J., Identification and characterization of regions of the rice genome associated with broad-spectrum, quantitative disease resistance. Genetics 169 (2005), 2277–2293.
15. [15] Deng, Y., Zhu, X., Shen, Y., He, Z., Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theor. Appl. Genet. 113 (2006), 705–713.
16. [16] Chen, S., Wang, L., Que, Z., Pan, R., Pan QGenetic and physical mapping of Pi37(t), a new gene conferring resistance to rice blast in the famous cultivar St. No. 1. Theor. Appl. Genet. 111 (2005), 1563–1570.
17. [17] Liu, X., Wang, L., Chen, S., Lin, F., Pan, Q., Genetic and physical mapping of Pi36 (t), a novel rice blast resistance gene located on rice chromosome 8. Mol. Genet. Genomics 274 (2005), 394–401.
18. [18] Fjellstrom, R., Conaway-Bormans, C.A., McClung, A.M., Marchetti, M.A., Shank, A.R., Park, W.D., Development of DNA markers suitable for marker assisted selection of three Pi Genes conferring resistance to multiple Pyricularia grisea pathotypes. Crop. Sci. 44 (2004), 1790–1798.
19. [19] Fjellstrom, R., McClung, A.M., Shank, A.R., SSR markers closely linked to the Pi-z locus are useful for selection of blast resistance in a broad array of rice germplasm. Mol. Breed. 17 (2006), 149–157.
20. [20] Hayashi, K., Yoshida, H., Ashikawa, I., Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theor. Appl. Genet. 113 (2006), 251–260.
21. [21] Hua, L., Wu, J., Chen, C., Wu, W., He, X., Lin, F., Wang, L., Ashikawa, I., Matsumoto, T., Wang, L., Pan, Q., The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast. Theor. Appl. Genet. 125 (2012), 1047–1055.
22. [22] Wang, Z., Yano, M., Yamanouchi, U., Iwamoto, M., Monna, L., Hayasaka, H., Katayose, Y., Sasaki, T., The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J. 19 (1999), 55–64.
23. [23] Bryan, G.T., Wu, K., Farrall, L., Jia, Y., Hershey, H.P., McAdams, S., Tarchini, R., Donaldson, G., Faulk, K., Valent, B., A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell 12 (2000), 2033–2045.
24. [24] Qu, S., Liu, G., Zhou, B., Bellizzi, M., Zeng, L., Dai, L., Han, B., Wang, G.L., The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics 172 (2006), 1901–1914.
25. [25] Zhou, B., Qu, S., Liu, G., Dolan, M., Sakai, H., Lu, G., Bellizzi, M., Wang, G.L., The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol. Plant Microbe Interact. 19 (2006), 1216–1228.
26. [26] Chen, X., Shang, J., Chen, D., Lei, C., Zou, Y., Zhai, W., Liu, G., Xu, J., Ling, Z., Cao, G., Ma, B., Wang, Y., Zhao, X., Li, S., Zhu, L., A B-lectin receptor kinase gene conferring rice blast resistance. Plant J. 46 (2006), 794–804.
27. [27] Liu, X., Lin, F., Wang, L., Pan, Q., The in silico map-based cloning of Pi36, a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus. Genetics 176 (2007), 2541–2549.
28. [28] Lin, F., Chen, S., Que, Z., Wang, L., Liu, X., Pan, Q., The blast resistance gene Pi37 encodes a nucleotide binding site-leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1. Genetics 177 (2007), 1871–1880.
29. [29] Ashikawa, I., Hayashi, N., Yamane, H., Kanamori, H., Wu, J., Matsumoto, T., Ono, K., Yano, M., Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics 180 (2008), 2267–2276.
30. [30] Lee, S.K., Song, M., Seo, Y.S., Kim, H.K., Ko, S., Cao, P., Suh, J.P., Yi, G., Roh, J.H., Lee, S., Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil–nucleotide-binding–leucine-rich repeat genes. Genetics 181 (2009), 1627–1638.
31. [31] Shang, J., Tao, Y., Chen, X., Zou, Y., Lei, C., Wang, J., Li, X., Zhao, X., Zhang, M., Lu, Z., Xu, J., Cheng, Z., Wan, J., Zhu, L., Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes. Genetics 182 (2009), 1303–1311.
32. [32] Chen, J., Shi, Y., Liu, W., Chai, R., Fu, Y., Zhuang, J., Wu, J., A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. J. Genet. Genomics 38 (2011), 209–216.
33. [33] Hayashi, K., Yoshida, H., Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter. Plant J. 57 (2009), 413–425.
34. [34] Takahashi, A., Hayashi, N., Miyao, A., Hirochika, H., Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging. BMC, Plant Pathol., 10, 2010, 175 ( http://www.biomedcentral.com/1471-2229/10/175).
35. [35] Fukuoka, S., Saka, N., Koga, H., Ono, K., Shimizu, T., Ebana, K., Hayashi, N., Takahashi, A., Hirochika, H., Okuno, K., Yano, M., Loss of Function of a proline-containing protein confers durable disease resistance in rice. Science 325 (2009), 998–1001.
36. [36] Hayashi, N., Inoue, H., Kato, T., Funao, T., Shirota, M., Shimizu, T., Kanamori, H., Yamane, H., Hayano-Saito, Y., Matsumoto, T., Yano, M., Takatsuji, H., Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J. 64 (2010), 498–510.
37. [37] Okuyama, Y., Kanzaki, H., Abe, A., Yoshida, K., Tamiru, M., Saitoh, H., Fujibe, T., Matsumura, H., Shenton, M., Galam, D.C., Undan, J., Ito, A., Sone, T., Terauchi, R., A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J. 66 (2011), 467–479.
38. [38] Cesari, S., Thilliez, G., Ribot, C., Chalvon, V., Michel, C., Rivas, S., Alaux, L., Kanzaki, H., Okuyama, Y., Morel, J.B., Fournier, E., Tharreau, D., Kroja, T., The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell 25 (2013), 1463–1481.
39. [39] Rai, A.K., Kumar, S.P., Gupta, S.K., Gautam, N., Singh, N.K., Sharma, T.R., Functional complementation of rice blast resistance gene Pi-kh(Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. J. Plant Biochem. Biot. 20 (2011), 55–65.
40. [40] Zhai, C., Lin, F., Dong, Z., He, X., Yuan, B., Zeng, X., Wang, L., Pan, Q., The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol. 189 (2011), 321–334.
41. [41] Yuan, B., Zhai, C., Wang, W., Zeng, X., Xu, X., Hu, H., Lin, F., Wang, L., Pan, Q., The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes. Theor. Appl. Genet. 122 (2011), 1017–1028.
42. [42] Jia, Y., Wang, Z., Singh, P., Development of dominant rice blast Pi-ta resistance gene markers. Crop. Sci. 42 (2002), 2145–2149.
43. [43] Jia, Y., Wang, Z., Fjellstrom, R.G., Moldenhauer, K.A.K., Azam, M.A., Correll, J., Lee, F.N., Xia, Y., Rutger, J.N., Rice Pi-ta gene confers resistance to the major pathotypes of the rice blast fungus in the United States. Phytopathology 94 (2004), 296–301.
44. [44] Dai, Z., Zhao, B., Liu, X., Xia, G., Tan, C., Zhang, B., Zhang, H., A new middle-mature indica rice variety Yangdao 6 with high yielding, high quality and multiple disease resistance. Jiangsu Agric. Sci. 4 (1997), 13–14 (in Chinese with English abstract).
45. 60Co-γirridation. Acta Agric. Nucl. Sin. 20:2 (2006), 83–86 (in Chinese with English abstract).
46. [46] Zhang, H., Dai, Z., Zhao, B., Kong, X., Xia, G., Li, A., Pan, C., Zhou, C., Wang, B., Huang, N., Tan, C., Zhu, Z., Construction of ideal plant type of an elite indica rice cultivar Yangdao 6 with high yield and good grain quality. J. Yangzhou Univ. (Agric. Life Sci. Ed.) 29:3 (2008), 1–6 (in Chinese with English abstract).
47. [47] Yang, Q., Lin, F., Wang, L., Pan, Q., Identification and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sativa subsp. indica reference cultivar 93-11. Theor. Appl. Genet. 118 (2009), 1027–1034.
48. [48] Huang, D., Characterization and SSR mapping of rice blast resistance genes. Postdoctoral Research Working Report of the Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 2005, 78 (in Chinese with English abstract).
49. [49] Wang, G.L., Mackill, D.J., Bonman, J.M., McCouch, S.R., Champoux, M.C., Nelson, R.J., RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136 (1994), 1421–1434.
50. [50] Liu, B., Zhang, S., Zhu, X., Yang, Q., Wu, S., Mei, M., Mouleon, R., Leach, J., New, T., Leung, H., Candidate defense genes as predictors of quantitative blast resistance in rice. Mol. Plant Microbe. Interact. 17 (2004), 1146–1152.
51. [51] Wu, J., Fan, Y., Li, D., Zheng, K., Zhuang, J., Leung, H., Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates. Theor. Appl. Genet. 111 (2005), 50–56.
52. [52] Wu, J., Liu, X., Dai, L., Wang, G., Advances on the identification and characterization of broad spectrum blast resistance genes in rice. Chin. Bull. Life Sci. 19 (2007), 233–238.
53. [53] Zhu, X., Chen, S., Yang, J., Zhou, S., Zeng, L., Han, J., Su, J., Wang, L., Pan, Q., The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor. Appl. Genet. 124 (2012), 1295–1304.
54. [54] Ling, Z., New, T., Wang, J., Lei, C., Huang, N., Development of near-isogenic lines of rice and their differential ability to the blast pathogen. Int. Rice Res. Notes 20 (1995), 13–14.
55. [55] Ling, Z., New, T., Wang, J., Lei, C., Huang, N., Development of Chinese near isogenic lines of rice and their differential ability of pathogenic races of blast fungus. Sci. Agric. Sin. 33:4 (2001), 1–8 (in Chinese with English abstract).
56. [56] Kobayashi, N., Telebanco-Yanoria, M.J., Tsunematsu, H., Kato, H., Imbe, T., Fukuta, Y., Development of new sets of international standard differential varieties for blast resistance in rice (Oryza sativa L.). Jpn. Agric. Res. Q 41 (2007), 31–37.
57. [57] Hayashi, N., Ando, I., Imbe, T., Identification of a new resistance gene to a Chinese blast fungus isolate in the Japanese rice cultivar Aichi Asahi. Phytopathology 88 (1998), 822–827.
58. [58] Kobayashi, N., Ebron, L.A., Fujita, D., Fukuta, Y., Identification of blast resistance genes in IRRI-bred rice varieties by segregation analysis based on a differential system. JIRCAS Work Rep. 63 (2009), 69–86.
59. [59] Sallaud, C., Lorieux, M., Roumen, E., Tharreau, D., Berruyer, R., Svestasrani, P., Garsmeur, O., Ghesquiere, A., Notteghem, J.L., Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theor. Appl. Genet. 106 (2003), 794–803.
60. [60] Chen, D., Zeigler, R.S., Ahn, S.W., Nelson, R.J., Phenotypic characterization of the rice blast resistance gene Pi2(t). Plant Dis. 80 (1996), 52–56.
61. [61] Lei, C., Wang, J., Mao, S., Zhu, L., Ling, Z., Gene analysis of blast resistance in indica variety Zhaiyeqing 8. Chin. J. Genet. 23 (1996), 273–277.
62. [62] Murray, M.G., Thompson, W.F., Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8 (1980), 4321–4325.
63. [63] Michelmore, R.W., Paran, I., Kesseli, R.V., Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. U.S.A. 88 (1991), 9828–9832.
64. [64] Zhang, Q., Shen, B.Z., Dai, X.K., Mei, M.H., Saghai Maroof M A, Li Z B. Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc. Natl. Acad. Sci. U.S.A. 91 (1994), 8675–8679.
65. [65] Sanguinetti, C.J., Dias, N.E., Simpson, A.J., Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17 (1994), 914–921.
66. [66] Gu, K., Tian, D., Yang, F., Wu, L., Sreekala, C., Wang, D., Wang, G.L., Yin, Z., High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in rice, Oryza sativa L. Theor. Appl. Genet. 108 (2004), 800–807.
67. [67] Kiyosawa, S., Ikehashi, H., Kato, H., Ling, Z., Pathogenicity test of Philippine isolates of blast fungus using two sets of rice varieties. Jpn. J. Breed 31 (1981), 367–376.
68. [68] Li, W., Lei, C., Cheng, Z., Jia, Y., Huang, D., Wang, J., Zhang, X., Su, N., Guo, X., Zhai, H., Wan, J., Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Mol. Breed. 22 (2008), 141–149.
69. [69] Liu, X., Yang, Q., Lin, F., Hua, L., Wang, C., Wang, L., Pan, Q., Identification and fine mapping of Pi39(t), a major gene conferring the broad-spectrum resistance to Magnaporthe oryzae. Mol. Genet. Genomics 278 (2007), 403–410.
70. [70] Kumar, P.K., Pathania, S., Katoch, P., Sharma, T.R., Plaha, P., Rathour, R., Genetic and physical mapping of blast resistance gene Pi-42(t) on the short arm of rice chromosome 12. Mol. Breed. 25 (2010), 217–228.
71. [71] Koide, Y., Telebanco-Yanoria, M.J., Fukuta, Y., Kobayashi, N., Detection of novel blast resistance genes, Pi58(t) and Pi59(t), in a Myanmar rice landrace based on a standard differential system. Mol. Breed., 2013, 10.1007/s11032-013-9865-5 (online).
72. [72] Inukai, T., Zeigler, R.S., Sarkarung, S., Bronson, M., Dung, L.V., Kinoshita, T., Nelson, R.J., Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population. Theor. Appl. Genet. 93 (1996), 560–567.
73. [73] Naqvi, N.I., Chattoo, B.B., Molecular genetic analysis and sequence characterized amplified region-assisted selection of blast resistance in rice. Rice Genet. 3 (1996), 570–576.
74. [74] Chen, D., dela Viña M, Inukai T, Mackill D J, Ronald P C, Nelson R J. Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar. Theor. Appl. Genet. 98 (1999), 1046–1053.
75. [75] Ahn, S.N., Kim, Y.K., Hong, H.C., Han, S.S., Kwon, S.J., Choi, H.C., Moon, H.P., McCouch, S.R., Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc). Euphytica 116 (2000), 17–22.
76. [76] Tabien, R.E., Li, Z., Paterson, A.H., Marchetti, M.A., Stansel, J.W., Pinson, S.R.M., Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’ and evaluation of their combinatorial effect for field resistance. Theor. Appl. Genet. 101 (2000), 1215–1225.
77. [77] Chen, X., Shang, J., Lei, C., Xu, J., Li, S., Zhu, L., Genetic and molecular analysis of blast resistance in a universal blast resistant variety, Digu. Wang, G.L., Valent, B., (eds.) Advances in Genetics, Genomics and Control of Rice Blast, 2009, Springer of Congress + Business Media B.V., USA, 149–159.
78. [78] Deng, Y., Zhu, X., Xu, J., Chen, H., He, Z., Map-based cloning and breeding application of a broad-spectrum resistance gene Pigm to rice blast. Wang, G.L., Valent, B., (eds.) Advances in Genetics, Genomics and Control of Rice Blast, 2009, Springer of Congress + Business Media B.V., USA, 161–171.
79. [79] Wu, J., Mizuno, H., Hayashi-Tsugane, M., Ito, Y., Chiden, Y., Fujisawa, M., Katagiri, S., Saji, S., Yoshiki, S., Karasawa, W., Physical maps and recombination frequency of six rice chromosomes. Plant J. 36 (2003), 720–730.