Superior isolation of antigen-specific brain infiltrating T cells using manual homogenization technique

Journal of Immunological Methods

Volumn 439, Issue , 2016, Pages 23-28

  Cumba Garcia, Luz M ^b   Huseby Kelcher, April M ^b   Malo, Courtney S ^b   Johnson, Aaron J ^a  

^a MAYO GRADUATE SCHOOL   (United States)

^b MAYO CLINIC   (United States)

Author keywords

CD8 T cell; CNS viral infection; Lymphocyte isolation; Neuroinflammation; Optimized recovery; Theiler's murine encephalomyelitis virus

Indexed keywords

CD45 ANTIGEN; EPITOPE; GELATINASE A; HERMES ANTIGEN; PADGEM PROTEIN; ANTIGEN; CAPSID PROTEIN; CD19 ANTIGEN; CD19 ANTIGEN, MOUSE; CD44 PROTEIN, MOUSE; COLLAGEN TYPE 4; COLLAGENASE; OVALBUMIN; PERCOLL; POVIDONE; SELECTIN; SILICON DIOXIDE; VP2 PROTEIN, THEILOVIRUS;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN INFECTION; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL SUSPENSION; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; IMMUNOLOGICAL PROCEDURES; INTERMETHOD COMPARISON; LYMPHOCYTIC INFILTRATION; MALE; MANUAL HOMOGENIZATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION; THEILER'S MURINE ENCEPHALOMYELITIS VIRUS; VIRUS INFECTION; ANIMAL; B LYMPHOCYTE; C57BL MOUSE; CARDIOVIRUS INFECTION; CELL SEPARATION; CELL SURVIVAL; CHEMISTRY; COMPARATIVE STUDY; DENSITY GRADIENT CENTRIFUGATION; DISEASE MODEL; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; PATHOGENICITY; PROCEDURES; THEILOVIRUS; VIROLOGY;

ANIMALS; ANTIGENS; ANTIGENS, CD19; ANTIGENS, CD44; B-LYMPHOCYTES; BRAIN; CAPSID PROTEINS; CARDIOVIRUS INFECTIONS; CD8-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CELL SURVIVAL; CENTRIFUGATION, DENSITY GRADIENT; COLLAGEN TYPE IV; COLLAGENASES; DISEASE MODELS, ANIMAL; EPITOPES; FEMALE; FLOW CYTOMETRY; LYMPHOCYTE ACTIVATION; MALE; MICE, INBRED C57BL; OVALBUMIN; POVIDONE; SELECTINS; SILICON DIOXIDE; THEILOVIRUS;

EID: 84997818372     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2016.09.002     Document Type: Article

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