An optimized method to process mouse CNS to simultaneously analyze neural cells and leukocytes by flow cytometry

Journal of Neuroscience Methods

Volumn 247, Issue , 2015, Pages 23-31

  Legroux, Laurine ^a,b   Pittet, Camille L ^a,b   Beauseigle, Diane ^a,b   Deblois, Gabrielle ^a,b   Prat, Alexandre ^a,b   Arbour, Nathalie ^a,b  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b CENTRE HOSPITALIER DE L UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Flow cytometry; Lymphocytes; Macrophages; Microglia; Neurons; Oligodendrocytes

Indexed keywords

CD11B ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN; CELL MARKER; COLLAGENASE; DEOXYRIBONUCLEASE I; LYMPHOCYTE SURFACE MARKER; NEURON SPECIFIC NUCLEAR PROTEIN; PAPAIN; TRYPSIN;

ADULT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; ENZYME DEGRADATION; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; FEMALE; FLOW CYTOMETRY; IMMUNOCOMPETENT CELL; LEUKOCYTE; MACROPHAGE; MICROGLIA; MOUSE; NERVE CELL; NONHUMAN; OLIGODENDROGLIA; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PROCESS OPTIMIZATION; T LYMPHOCYTE; TISSUE PREPARATION; ANIMAL; C57BL MOUSE; CYTOLOGY; PROCEDURES;

ANIMALS; CENTRAL NERVOUS SYSTEM; FLOW CYTOMETRY; LEUKOCYTES; MICE; MICE, INBRED C57BL; NEURONS;

EID: 84926377213     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2015.03.021     Document Type: Article

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