Quantifying autophagy: Measuring LC3 puncta and autolysosome formation in cells using multispectral imaging flow cytometry

Methods

Volumn 112, Issue , 2017, Pages 147-156

  Pugsley, Haley R ^a  

^a EMD Millipore   (United States)

Author keywords

Autolysosome; Autophagosome; Autophagy; ImageStream; LC3; Multispectral imaging flow cytometry (MIFC)

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN 1; MICROTUBULE ASSOCIATED PROTEIN 1A; UNCLASSIFIED DRUG; ALEXA FLUOR 647; ANTIBODY; CARBOCYANINE; CHLOROQUINE; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; LAMP1 PROTEIN, HUMAN; LIGHT CHAIN 3, HUMAN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN; MICROTUBULE ASSOCIATED PROTEIN;

ANALYTIC METHOD; AUTOLYSOSOME; AUTOPHAGY; BRIGHT DETAIL DENSITY; BRIGHT DETAIL SIMILARITY; BRIGHT DETAIL SIMILARITY AND SPOT COUNT; CELL COUNTING; CELL CULTURE; CELL LABELING; CELLULAR DISTRIBUTION; FLOW CYTOMETER; FLOW CYTOMETRY; HUMAN; IMAGE ANALYSIS; IMAGING SOFTWARE; MULTISPECTRAL IMAGING FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; REVIEW; SPOT COUNT; AUTOPHAGOSOME; CHEMISTRY; DEVICES; DRUG EFFECTS; GENETICS; IMAGE CYTOMETRY; JURKAT CELL LINE; LYSOSOME; METABOLISM; PROCEDURES; STAINING;

ANTIBODIES; AUTOPHAGOSOMES; AUTOPHAGY; CARBOCYANINES; CHLOROQUINE; FLOW CYTOMETRY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE CYTOMETRY; JURKAT CELLS; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; LYSOSOMES; MICROTUBULE-ASSOCIATED PROTEINS; STAINING AND LABELING;

EID: 84995599607     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2016.05.022     Document Type: Review

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