LC3 and autophagy

Methods in Molecular Biology

Volumn 445, Issue , 2008, Pages 77-88

  Tanida, Isei ^a   Ueno, Takashi ^a   Kominami, Eiki ^a  

^a JUNTENDO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

ATG conjugation system; Autolysosome; Autophagosome; Autophagy; LC3; Lipidation; Ubiquitylation like reaction

Indexed keywords

MAMMALIA;

EID: 84934440388     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-157-4_4     Document Type: Article

References (19)

1. Eskelinen, E. L. (2005) Maturation of autophagic vacuoles in mammalian cells. Autophagy 1, 1-10.
2. Kadowaki, M., Karim, M. R., Carpi, A., and Miotto, G. (2006) Nutrient control of macroautophagy in mammalian cells. Mol. Aspects Med. 27, 426-443.
3. Wileman, T. (2006) Aggresomes and autophagy generate sites for virus replication. Science 312, 875-878.
4. Rubinsztein, D. C., Difiglia, M., Heintz, N., et al. (2005) Autophagy and its possible roles in nervous system diseases, damage and repair. Autophagy 1, 11-22.
5. Munz, C. (2006) Autophagy and antigen presentation. Cell Microbiol. 8, 891-898.
6. Deretic, V., Singh, S., Master, S., et al. (2006) Mycobacterium tuberculosis inhibition of phagolysosome biogenesis and autophagy as a host defence mechanism. Cell Microbiol. 8, 719-727.
7. Mann, S. S., and Hammarback, J. A. (1994) Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B. J. Biol. Chem. 269, 11492-11497.
8. Kabeya, Y., Mizushima, N., Ueno, T., et al. (2000) LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19, 5720-5728.
9. Tañida, I., Minematsu-Ikeguchi, N., Ueno, T., and Kominami, E. (2005) Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1, 84-91.
10. Kabeya, Y., Mizushima, N., Yamamoto, A., Oshitani-Okamoto, S., Ohsumi, Y., and Yoshimori, T. (2004) LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. J. Cell Sci. 117, 2805-2812.
11. Sou, Y. S., Tañida, I., Komatsu, M., Ueno, T., and Kominami, E. (2006) Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16. J. Biol. Chem. 281, 3017-3024.
12. Tañida, I., Tanida-Miyake, E., Ueno, T., and Kominami, E. (2001) The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAPLC3. J. Biol. Chem. 276, 1701-1706.
13. Tañida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., and Kominami, E. (2002) Human Apg3p/Autlp homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J. Biol. Chem. 277, 13739-13744.
14. Tañida, I., Ueno, T., and Kominami, E. (2004) LC3 conjugation system in mammalian autophagy. Int. J. Biochem. Cell Biol. 36, 2503-2518.
15. Ueno, T., Muno, D., and Kominami, E. (1991) Membrane markers of endoplasmic reticulum preserved in autophagic vacuolar membranes isolated from leupeptinadministered rat liver. J. Biol. Chem. 266, 18995-18999.
16. Ueno, T., Ishidoh, K., Mineki, R., et al. (1999) Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. J. Biol. Chem. 274, 15222-15229.
17. Tamai, M., Matsumoto, K., Omura, S., Koyama, I., Ozawa, Y., and Hanada, K. (1986) In vitro and in vivo inhibition of cysteine proteinases by EST, a new analog of E-64. J. Pharmacobiodyn. 9, 672-677.
18. Umezawa, H., Takeuchi, T., Linuma, H., Suzuki, K., and Ito, M. (1970) A new microbial product, oudenone, inhibiting tyrosine hydroxylase. J. Antibiot. (Tokyo) 23, 514-518.
19. Jager, S., Bucci, C., Tañida, I., et al. (2004) Role for Rab7 in maturation of late autophagic vacuoles. J. Cell Sci. 117, 4837-4848.