Trehalose prevents aggregation of exosomes and cryodamage

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Bosch, Steffi ^a   De Beaurepaire, Laurence ^a   Allard, Marie ^a   Mosser, Mathilde ^a   Heichette, Claire ^b   Chrétien, Denis ^b,c   Jegou, Dominique ^a   Bach, Jean Marie ^a  

^a UNIVERSITÉ DE NANTES   (France)

^b UNIVERSITÉ DE RENNES 1   (France)

^c UNIVERSITÉ DE RENNES 1   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CRYOPROTECTIVE AGENT; TREHALOSE;

CELL LINE; CRYOPRESERVATION; EXOSOME; HUMAN; METABOLISM; PANCREAS ISLET BETA CELL; ULTRASTRUCTURE;

CELL LINE; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; EXOSOMES; HUMANS; INSULIN-SECRETING CELLS; TREHALOSE;

EID: 84994400006     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep36162     Document Type: Article

References (50)

1. Harding, C., Heuser, J. & Stahl, P. Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes. Journal of Cell Biology 97, 329-339, doi: 10.1083/jcb.97.2.329 (1983).
2. Pan, B. T. & Johnstone, R. M. Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro-selective externalization of the receptor. Cell 33, 967-977, doi: 10.1016/0092-8674(83)90040-5 (1983).
3. Raposo, G. et al. B lymphocytes secrete antigen-presenting vesicles. J Exp Med. 183, 1161-1172 (1996).
4. Ramachandra, L. et al. Mycobacterium tuberculosis Synergizes with ATP To Induce Release of Microvesicles and Exosomes Containing Major Histocompatibility Complex Class II Molecules Capable of Antigen Presentation. Infection and Immunity 78, 5116-5125, doi: 10.1128/iai.01089-09 (2010).
5. Skog, J. et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nature Cell Biology 10, 1470-U1209, doi: 10.1038/ncb1800 (2008).
6. Yanez-Mo, M. et al. Biological properties of extracellular vesicles and their physiological functions. Journal of Extracellular Vesicles 4, doi: 10.3402/jev.v4.27066 (2015).
7. Salama, A. et al. MicroRNA-29b Modulates Innate and Antigen-Specific Immune Responses in Mouse Models of Autoimmunity. Plos One 9, doi: 10.1371/journal.pone.0106153 (2014).
8. Théry, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. Chapter 3, Unit 3.22, doi: 10.1002/0471143030.cb0322s30 (2006).
9. Lasser, C., Eldh, M. & Lotvall, J. Isolation and characterization of RNA-containing exosomes. Journal of visualized experiments: JoVE e3037, doi: 10.3791/3037 (2012).
10. Witwer, K. W. et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. Journal of extracellular vesicles 2, doi: 10.3402/jev.v2i0.20360 (2013).
11. Jain, N. K. & Roy, I. Effect of trehalose on protein structure. Protein Science 18, 24-36, doi: 10.1002/pro.3 (2009).
12. Eroglu, A., Toner, M. & Toth, T. L. Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes. Fertility and Sterility 77, 152-158, doi: 10.1016/s0015-0282(01)02959-4 (2002).
13. Katenz, E. et al. Cryopreservation of primary human hepatocytes: The benefit of trehalose as an additional cryoprotective agent. Liver Transplantation 13, 38-45, doi: 10.1002/lt.20921 (2007).
14. Ikeda, M. et al. Clinical application of ET-Kyoto solution for lung transplantation. Surgery Today 45, 439-443, doi: 10.1007/s00595-014-0918-0 (2015).
15. Beattie, G. M. et al. Trehalose: A cryoprotectant that enhances recovery and preserves function of human pancreatic islets after longterm storage. Diabetes 46, 519-523, doi: 10.2337/diabetes.46.3.519 (1997).
16. Kim, Y.-C. et al. Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles. 3rd Vaccine Global Congress, Singapore 2009 2, 17-21, doi: 10.1016/j.provac.2010.03.004 (2010).
17. Richards, A. B. et al. Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food and Chemical Toxicology 40, 871-898, doi: 10.1016/s0278-6915(02)00011-x (2002).
18. Sato, S., Okamoto, K., Minami, R., Kohri, H. & Yamamoto, S. Trehalose can be used as a parenteral saccharide source in rabbits. Journal of Nutrition 129, 158-164 (1999).
19. Beranger, F., Crozet, C., Goldsborough, A. & Lehmann, S. Trehalose impairs aggregation of PrPSc molecules and protects prioninfected cells against oxidative damage. Biochemical and Biophysical Research Communications 374, 44-48, doi: 10.1016/j. bbrc.2008.06.094 (2008).
20. Liu, R., Barkhordarian, H., Emadi, S., Park, C. B. & Sierks, M. R. Trehalose differentially inhibits aggregation and neurotoxicity of beta-amyloid 40 and 42. Neurobiology of Disease 20, 74-81, doi: 10.1016/j.nbd.2005.02.003 (2005).
21. Tanaka, M. et al. Trehalose alleviates polyglutamine-mediated pathology in a mouse model of Huntington disease. Nature Medicine 10, 148-154, doi: 10.1038/nm985 (2004).
22. Maki, K. C., Kanter, M., Rains, T. M., Hess, S. P. & Geohas, J. Acute effects of low insulinemic sweeteners on postprandial insulin and glucose concentrations in obese men. International Journal of Food Sciences and Nutrition 60, 48-55, doi: 10.1080/09637480802646923 (2009).
23. Hood, J. L., Scott, M. J. & Wickline, S. A. Maximizing exosome colloidal stability following electroporation. Analytical Biochemistry 448, 41-49, doi: 10.1016/j.ab.2013.12.001 (2014).
24. Miyazaki, J. I. et al. Establishment of a pancreatic beta-cell line that retains glucose-inducible insulin-secretion - special reference to expression of glucose transporter isoforms. Endocrinology 127 (1990).
25. Gardiner, C., Ferreira, Y. J., Dragovic, R. A., Redman, C. W. G. & Sargent, I. L. Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. Journal of extracellular vesicles 2, doi: 10.3402/jev.v2i0.19671 (2013).
26. Gyoergy, B. et al. Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. Cellular and Molecular Life Sciences 68, 2667-2688, doi: 10.1007/s00018-011-0689-3 (2011).
27. Van Deun, J. et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. Journal of extracellular vesicles 3, doi: 10.3402/jev.v3.24858 (2014).
28. Sheng, H. et al. Insulinoma-Released Exosomes or Microparticles Are Immunostimulatory and Can Activate Autoreactive T Cells Spontaneously Developed in Nonobese Diabetic Mice. Journal of Immunology 187, 1591-1600, doi: 10.4049/jimmunol.1100231 (2011).
29. Kim, S. H. et al. Exosomes derived from IL-10-treated dendritic cells can suppress inflammation and collagen-induced arthritis. Journal of Immunology 174, 6440-6448 (2005).
30. Bashratyan, R., Sheng, H., Regn, D., Rahman, M. J. & Dai, Y. D. Insulinoma-released exosomes activate autoreactive marginal zonelike B cells that expand endogenously in prediabetic NOD mice. European Journal of Immunology 43, 2588-2597, doi: 10.1002/eji.201343376 (2013).
31. Zhou, H. et al. Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney International 69, 1471-1476, doi: 10.1038/sj.ki.5000273 (2006).
32. Linares, R., Tan, S., Gounou, C., Arraud, N. & Brisson, A. R. High-speed centrifugation induces aggregation of extracellular vesicles. Journal of Extracellular Vesicles 4, doi: 10.3402/jev.v4.29509 (2015).
33. Kirkegaard, J. S. et al. Xenotropic retrovirus Bxv1 in human pancreatic beta cell lines. Journal of Clinical Investigation 126, 1109-1113, doi: 10.1172/jci83573 (2016).
34. Willms, E. et al. Cells release subpopulations of exosomes with distinct molecular and biological properties. Scientific Reports 6, doi: 10.1038/srep22519 (2016).
35. Bobrie, A., Colombo, M., Krumreich, S., Raposo, G. & Théry, C. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. Journal of Extracellular Vesicles 1, doi: 10.3402/jev.v1i0.18397 (2012).
36. Hoog, J. L. & Lotvall, J. Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. Journal of Extracellular Vesicles 4, doi: 10.3402/jev.v4.28680 (2015).
37. Arraud, N. et al. Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration. Journal of Thrombosis and Haemostasis 12, 614-627, doi: 10.1111/jth.12554 (2014).
38. Webber, J. & Clayton, A. How pure are your vesicles? Journal of extracellular vesicles 2, doi: 10.3402/jev.v2i0.19861 (2013).
39. Marimpietri, D. et al. Proteome Profiling of Neuroblastoma-Derived Exosomes Reveal the Expression of Proteins Potentially Involved in Tumor Progression. Plos One 8, doi: 10.1371/journal.pone.0075054 (2013).
40. Smith, Z. J. et al. Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. Journal of Extracellular Vesicles 4, doi: 10.3402/jev.v4.28533 (2015).
41. Yang, L. et al. Exogenous trehalose largely alleviates ionic unbalance, ROS burst, and PCD occurrence induced by high salinity in Arabidopsis seedlings. Frontiers in Plant Science 5, doi: 10.3389/fpls.2014.00570 (2014).
42. Chang, B., Yang, L., Cong, W., Zu, Y. & Tang, Z. The improved resistance to high salinity induced by trehalose is associated with ionic regulation and osmotic adjustment in Catharanthus roseus. Plant Physiology and Biochemistry 77, 140-148, doi: 10.1016/j. plaphy.2014.02.001 (2014).
43. Reina-Bueno, M. et al. Role of Trehalose in Salinity and Temperature Tolerance in the Model Halophilic Bacterium Chromohalobacter salexigens. Plos One 7, doi: 10.1371/journal.pone.0033587 (2012).
44. Sokolova, V. et al. Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids and Surfaces B-Biointerfaces 87, 146-150, doi: 10.1016/j.colsurfb.2011.05.013 (2011).
45. Jayachandran, M., Miller, V. M., Heit, J. A. & Owen, W. G. Methodology for isolation, identification and characterization of microvesicles in peripheral blood. Journal of Immunological Methods 375, 207-214, doi: 10.1016/j.jim.2011.10.012 (2012).
46. Tanaka, K. et al. Trehalose does not affect the functions of human neutrophils in vitro. Surgery Today 44, 332-339, doi: 10.1007/s00595-013-0625-2 (2014).
47. Echigo, R. et al. Trehalose treatment suppresses inflammation, oxidative stress, and vasospasm induced by experimental subarachnoid hemorrhage. Journal of Translational Medicine 10, doi: 10.1186/1479-5876-10-80 (2012).
48. Duchesne, L., Gentili, D., Comes-Franchini, M. & Fernig, D. G. Robust Ligand Shells for Biological Applications of Gold Nanoparticles. Langmuir 24, 13572-13580, doi: 10.1021/la802876u (2008).
49. Mastronarde, D. N. Dual-axis tomography: An approach with alignment methods that preserve resolution. Journal of Structural Biology 120, 343-352, doi: 10.1006/jsbi.1997.3919 (1997).
50. Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. Journal of Structural Biology 116, 71-76, doi: 10.1006/jsbi.1996.0013 (1996).