High-speed centrifugation induces aggregation of extracellular vesicles

Journal of Extracellular Vesicles

Volumn 4, Issue 1, 2015, Pages

  Linares, Romain ^a   Tan, Sisareuth ^a   Gounou, Céline ^a   Arraud, Nicolas ^a   Brisson, Alain R ^a,b  

^a UNIVERSITÉ DE BORDEAUX   (France)

^b INSTITUT UNIVERSITAIRE DE FRANCE   (France)

Author keywords

Blood plasma; Centrifugation; Cryo electron microscopy; Extracellular vesicles; Flow cytometry; Immuno gold electron microscopy; Vesicle aggregation

Indexed keywords

ANTIGEN PURIFICATION; BLOOD-TO-PLASMA RATIO; BODY FLUID; CELL AGGREGATION; CELL FUNCTION; CELL ISOLATION; CELL STRUCTURE; CENTRIFUGATION; CRYOELECTRON MICROSCOPY; CYTOPLASM; EXOSOME; HUMAN; NORMAL HUMAN; PHENOTYPE; SHORT SURVEY; THROMBOCYTE FREE PLASMA;

EID: 84983549222     PISSN: None     EISSN: 20013078     Source Type: Journal    
DOI: 10.3402/jev.v4.29509     Document Type: Short Survey

References (41)

1. Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013;200:373-83
2. Yuana Y, Sturk A, Nieuwland R. Extracellular vesicles in physiological and pathological conditions. Blood Rev. 2013;27:31-9
3. Kowal J, Tkach M, Théry C. Biogenesis and secretion of exosomes. Curr Opin Cell Biol. 2014;29:116-25
4. Gould SJ, Raposo G. As we wait: coping with an imperfect nomenclature for extracellular vesicles. J Extracell Vesicles. 2013;2:20309. doi: http://dx.doi.org/10.3402/jev.v2i0.20389
5. Witwer KW, Buzás EI, Bemis LT, Bora A, Lässer C, Lötvall J, et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles. 2013;2:20360. doi: http://dx.doi.org/10.3402/jev.v2i0.20360
6. Raposo G, Nijman HW, Stoorvogel W, Liejendekker R, Harding CV, Melief CJ, et al. B lymphocytes secrete antigenpresenting vesicles. J Exp Med. 1996;1161-72
7. Pasquet JM, Toti F, Nurden AT, Dachary-Prigent J. Procoagulant activity and active calpain in platelet-derived microparticles. Thromb Res. 1996;87:509-22
8. Nieuwland R, Berckmans RJ, Rotteveel-Eijkman RC, Maquelin KN, Roozendaal KJ, Jansen PGM, et al. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. 1997;96:35, 34-41
9. Shet AS, Aras O, Gupta K, Hass MJ, Rausch DJ, Saba N, et al. Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes. Blood. 2003; 102:2678-83
10. Shet AS, Key NS, Hebbel RP. Measuring circulating cellderived microparticles. J Thromb Haemost. 2004;2:1848-50
11. Théry C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006;Chapter 3:1-29
12. Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ, Lötvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007;9:654-9
13. Leroyer AS, Isobe H, Leseche G, Castier Y, Wassef M, Mallat Z, et al. Cellular origins and thrombogenic activity of microparticles isolated from human atherosclerotic plaques. J Am Coll Cardiol. 2007;49:772-7
14. Dasgupta SK, Abdel-Monem H, Niravath P, Le A, Bellera RV, Langlois K, et al. Lactadherin and clearance of plateletderived microvesicles. Blood. 2009;113:1332-9
15. György B, Módos K, Pállinger É, Pálóczi K, Pásztó i M, Misják P, et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood. 2011;117:e39-48
16. Peinado H, Alec?ković M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med. 2012;18:883-91
17. Tushuizen ME, Diamant M, Peypers EG, Hoek FJ, Heine RJ, Sturk A, et al. Postprandial changes in the phospholipid composition of circulating microparticles are not associated with coagulation activation. Thromb Res. 2012;130:115-21
18. Yuana Y, Koning RI, Kuil ME, Rensen PCN, Koster AJ, Bertina RM, et al. Cryo-electron microscopy of extracellular vesicles in fresh plasma. J Extracell Vesicles. 2013;2:21494. doi: http://dx.doi.org/10.3402/jev.v2i0.21494
19. Varga Z, Yuana Y, Grootemaat AE, van der Pol E, Gollwitzer C, Krumrey M, et al. Towards traceable size determination of extracellular vesicles. J Extracell Vesicles. 2014;3:23298. doi: http://dx.doi.org/10.3402/jev.v3.23298
20. Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, et al. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature. 2015;523:177-82
21. Issman L, Brenner B, Talmon Y, Aharon A. Cryogenic transmission electron microscopy nanostructural study of shed microparticles. PLoS One. 2013;8:e83680
22. Erdbrügger U, Rudy CK, E Etter M, Dryden KA, Yeager M, Klibanov AL, et al. Imaging flow cytometry elucidates limitations of microparticle analysis by conventional flow cytometry. Cytometry A. 2014;85:756-70
23. Arraud N, Linares R, Tan S, Gounou C, Pasquet J-M, Mornet S, et al. Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration. J Thromb Haemost. 2014;12:614-27
24. György B, Szabó TG, Pásztó i M, Pál Z, Misják P, Aradi B, et al. Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles. CellMol Life Sci. 2011;68:2667-88
25. Van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R. Classification, functions, and clinical relevance of extracellular vesicles. Pharmacol Rev. 2012;64:676-705
26. Jy W, Horstman LL, Jimenez JJ, Ahn YS, Biró E, Nieuwland R, et al. Measuring circulating cell-derived microparticles. J Thromb Haemost. 2004;2:1842-51
27. Lacroix R, Judicone C, Poncelet P, Robert S, Arnaud L, Sampol J, et al. Impact of pre-analytical parameters on the measurement of circulating microparticles: towards standardization of protocol. J Thromb Haemost. 2012;10:437-46
28. Lyon ME, Fine JS, Henderson PJ, Lyon AW. D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK): alternative anticoagulant to heparin salts for blood gas and electrolyte specimens. Clin Chem. 1995;41:1038-41
29. Arraud N, Gounou C, Turpin D, Brisson AR. Fluorescence triggering: a general strategy for enumerating and phenotyping extracellular vesicles by flow cytometry. Cytometry A. 2015. doi: http://dx.doi.org/10.1002/cyto.a.22669. [Epub ahead of print]
30. Arraud N, Gounou C, Linares R, Brisson AR. A simple flow cytometry method improves the detection of phosphatidylserineexposing extracellular vesicles. J Thromb Haemost. 2015;13: 237-47
31. Van der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG, Nieuwland R. Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost. 2010;8:2596-607
32. Chandler WL, Yeung W, Tait JF. A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost. 2011; 9:1216-24
33. Diamant M, Nieuwland R, Pablo RF, Sturk A, Smit JWA, Radder JK. Elevated numbers of tissue-factor exposing microparticles correlate with components of the metabolic syndrome in uncomplicated type 2 diabetes mellitus. Circulation. 2002;106:2442-7
34. Trappenburg MC, van Schilfgaarde M, Marchetti M, Spronk HM, ten Cate H, Leyte A, et al. Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombocythemia. Haematologica. 2009;94:911-8
35. Tesselaar MET, Romijn FPHTM, Van Der Linden IK, Prins FA, Bertina RM, Osanto S. Microparticle-associated tissue factor activity: a link between cancer and thrombosis? J Thromb Haemost. 2007;5:520-7
36. Nieuwland R. Cellular origin of microparticles exposing tissue factor in cancer: a mixed double? J Thromb Haemost. 2008;6: 1514-6
37. Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles. 2014;3:24858. doi: http://dx.doi.org/10.3402/jev.v3.24858
38. Maas SLN, de Vrij J, van der Vlist EJ, Geragousian B, van Bloois L, Mastrobattista E, et al. Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics. J Control Release. 2015; 200:87-96
39. Momen-Heravi F, Balaj L, Alian S, Mantel P-Y, Halleck AE, Trachtenberg AJ, et al. Current methods for the isolation of extracellular vesicles. Biol Chem. 2013;394:1253-62
40. Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 2014;3:23430. doi: http://dx.doi.org/10.3402/jev.v3.23430
41. Arakelyan A, Ivanova O, Vasilieva E, Grivel J-C, Margolis L. Antigenic composition of single nano-sized extracellular blood vesicles. Nanomedicine. 2015;11:489-98