Design and validation of CRISPR/cas9 systems for targeted gene modification in induced pluripotent stem cells

Methods in Molecular Biology

Volumn 1498, Issue , 2017, Pages 3-21

  Lee, Ciaran M ^a   Zhu, Haibao ^a   Davis, Timothy H ^a   Deshmukh, Harshahardhan ^a   Bao, Gang ^a  

^a Rice University   (United States)

Author keywords

CRISPR; Genome editing; Targeted gene knockout

Indexed keywords

GENOMIC DNA;

CONFERENCE PAPER; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA EXTRACTION; DNA MODIFICATION; DNA SEQUENCE; DNA TEMPLATE; FEEDER CELL; FLOW CYTOMETRY; GENE TARGETING; GENETIC CODE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INDUCED MUTATION; INDUCED PLURIPOTENT STEM CELL; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE EDITING; GENETICS; HEK293 CELL LINE; HUMAN GENOME; PHYSIOLOGY; PLURIPOTENT STEM CELL; PROCEDURES; RNA EDITING;

CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; GENE EDITING; GENOME, HUMAN; HEK293 CELLS; HUMANS; PLURIPOTENT STEM CELLS; RNA EDITING;

EID: 84990985657     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6472-7_1     Document Type: Conference Paper

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