Clean fractionation pretreatment reduces enzyme loadings for biomass saccharification and reveals the mechanism of free and cellulosomal enzyme synergy

ACS Sustainable Chemistry and Engineering

Volumn 2, Issue 6, 2014, Pages 1377-1387

  Resch, Michael G ^a   Donohoe, Bryon S ^a   Ciesielski, Peter N ^a   Nill, Jennifer E ^a   Magnusson, Lauren ^a   Himmel, Michael E ^a   Mittal, Ashutosh ^a   Katahira, Rui ^a   Biddy, Mary J ^a   Beckham, Gregg T ^a  

^a NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

Author keywords

Biofuels; Cellulase; Cellulose; Lignin; Organosolv

Indexed keywords

ACETONE; BIOCONVERSION; BIOFUELS; BIOMASS; CELLULOSE; LIGNIN; POLYSACCHARIDES; SACCHARIFICATION; SCANNING ELECTRON MICROSCOPY; SUBSTRATES; SULFURIC ACID;

CELLULASE; DILUTE SULFURIC ACID; ENZYMATIC DEPOLYMERIZATION; ENZYMATIC SACCHARIFICATION; LIGNOCELLULOSIC BIOMASS; ORGANOSOLV; ORGANOSOLV PRETREATMENT; RESIDUAL HEMICELLULOSE;

ENZYMES;

CELLULASE; CELLULOSE; LIGNINS; ORGANOSOLV LIGNINS;

EID: 84990872349     PISSN: None     EISSN: 21680485     Source Type: Journal    
DOI: 10.1021/sc500210w     Document Type: Article

References (58)

1. Chundawat, S. P. S.; Beckham, G. T.; Himmel, M. E.; Dale, B. E. Deconstruction of lignocellulosic biomass to fuels and chemicals. Annu. Rev. Chem. Biomol. Eng. 2011, 2, 6.1-6.25
2. Himmel, M. E.; Ding, S. Y.; Johnson, D. K.; Adney, W. S.; Nimlos, M. R.; Brady, J. W.; Foust, T. D. Biomass recalcitrance: Engineering plants and enzymes for biofuels production. Science 2007, 315, 804-807
3. Mosier, N.; Wyman, C.; Dale, B.; Elander, R.; Lee, Y. Y.; Holtzapple, M.; Ladisch, M. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 2005, 96, 673-686
4. Aden, A.; Foust, T. Technoeconomic analysis of the dilute sulfuric acid and enzymatic hydrolysis process for the conversion of corn stover to ethanol. Cellulose 2009, 16, 535-545
5. Davis, R.; Tao, L.; Tan, E.; Biddy, M. J.; Beckham, G. T.; Scarlata, C.; Jacobson, J.; Cafferty, K.; Ross, J.; Lukas, J., et al. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid Prehydrolysis and Enzymatic Hydrolysis Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons; Technical Report NREL/TP-5100-6022; National Renewable Energy Laboratory: Golden, CO, 2013
6. Humbird, D.; Davis, R.; Tao, L.; Kinchin, C.; Hsu, D.; Aden, A.; Schoen, P.; Lukas, J.; Olthof, B.; Worley, M., et al. Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol; Technical Report NREL/TP-5100-47764; National Renewable Energy Laboratory: Golden, CO, 2011
7. Tao, L.; Aden, A.; Elander, R. T.; Pallapolu, V. R.; Lee, Y. Y.; Garlock, R. J.; Balan, V.; Dale, B. E.; Kim, Y.; Mosier, N. S.; et al. Process and technoeconomic analysis of leading pretreatment technologies for lignocellulosic ethanol production using switchgrass. Bioresour. Technol. 2011, 102, 11105-11114
8. Vicari, K. J.; Tallam, S. S.; Shatova, T.; Joo, K. K.; Scarlata, C. J.; Humbird, D.; Wolfrum, E. J.; Beckham, G. T., Uncertainty in technoeconomic estimates of cellulosic ethanol production due to experimental measurement uncertainty. Biotechnol. Biofuels 2012, 5
9. Martinez, D.; Berka, R. M.; Henrissat, B.; Saloheimo, M.; Arvas, M.; Baker, S. E.; Chapman, J.; Chertkov, O.; Coutinho, P. M.; Cullen, D.; et al. Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat. Biotechnol. 2008, 26, 553-560
10. Jalak, J.; Kurasin, M.; Teugjas, H.; Väljamäe, P. Endo-exo synergism in cellulose hydrolysis revisited. J. Biol. Chem. 2012, 287, 28802-28815
11. Vaaje-Kolstad, G.; Westereng, B.; Horn, S. J.; Liu, Z. L.; Zhai, H.; Sorlie, M.; Eijsink, V. G. H. An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science 2010, 330, 219-222
12. Quinlan, R. J.; Sweeney, M. D.; Lo Leggio, L.; Otten, H.; Poulsen, J. C. N.; Johansen, K. S.; Krogh, K.; Jorgensen, C. I.; Tovborg, M.; Anthonsen, A.; et al. Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components. Proc. Natl. Acad. Sci. U.S.A. 2011, 108, 15079-15084
13. Phillips, C. M.; Beeson, W. T.; Cate, J. H.; Marletta, M. A. Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa. ACS Chem. Biol. 2011, 6, 1399-1406
14. Westereng, B.; Ishida, T.; Vaaje-Kolstad, G.; Wu, M.; Eijsink, V. G.; Igarashi, K.; Samejima, M.; Stahlberg, J.; Horn, S. J.; Sandgren, M. The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose. PloS one 2011, 6, e27807
15. Kim, S.; Stahlberg, J.; Sandgren, M.; Paton, R. S.; Beckham, G. T. Quantum mechanical calculations suggest that lytic polysaccharide monooxygenases use a copper-oxyl, oxygen-rebound mechanism. Proc. Natl. Acad. Sci. U.S.A. 2014, 111, 149-154
16. Agger, J. W.; Isaksen, T.; Varnai, A.; Vidal-Melgosa, S.; Willats, W. G.; Ludwig, R.; Horn, S. J.; Eijsink, V. G.; Westereng, B. Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation. Proc. Natl. Acad. Sci. U.S.A. 2014, 111, 6287-6292
17. Lombard, V.; Golaconda Ramulu, H.; Drula, E.; Coutinho, P. M.; Henrissat, B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res. 2014, 42, D490-D495
18. Bayer, E. A.; Chanzy, H.; Lamed, R.; Shoham, Y. Cellulose, cellulases and cellulosomes. Curr. Opin. Struct. Biol. 1998, 8, 548-57
19. Fontes, C. M.; Gilbert, H. J. Cellulosomes: Highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu. Rev. Biochem. 2010, 79, 655-81
20. Demain, A. L.; Newcomb, M.; Wu, J. H. D. Cellulase, clostridia, and ethanol. Microbiol. Mol. Biol. Rev. 2005, 69, 124-+
21. Olson, D. G.; McBride, J. E.; Shaw, A. J.; Lynd, L. R. Recent progress in consolidated bioprocessing. Curr. Opin Biotechnol 2012, 23, 396-405
22. Raman, B.; Pan, C.; Hurst, G. B.; Rodriguez, M.; McKeown, C. K.; Lankford, P. K.; Samatova, N. F.; Mielenz, J. R. Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: A quantitative proteomic analysis. PloS One 2009, Doi: 10.1371/journal.-pone.0005271
23. Fontes, C. M. G. A.; Gilbert, H. J. Cellulosomes: Highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu. Rev. Biochem. 2010, 79, 655-681
24. Bayer, E. A.; Belaich, J. P.; Shoham, Y.; Lamed, R. The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Annu. Rev. Microbiol. 2004, 58, 521-54
25. Chanzy, H.; Henrissat, B. Undirectional degradation of Valonia cellulose microcrystals subjected to cellulase action. FEBS Lett. 1985, 184, 285-288
26. Imai, T.; Boisset, C.; Samejima, M.; Igarashi, K.; Sugiyama, J. Unidirectional processive action of cellobiohydrolase Cel7A on Valonia cellulose microcrystals. FEBS Lett. 1998, 432, 113-6
27. Boisset, C.; Chanzy, H.; Henrissat, B.; Lamed, R.; Shoham, Y.; Bayer, E. A. Digestion of crystalline cellulose substrates by the Clostridium thermocellum cellulosome: Structural and morphological aspects. Biochem. J. 1999, 340, 829-835
28. Boisset, C.; Fraschini, C.; Schulein, M.; Henrissat, B.; Chanzy, H. Imaging the enzymatic digestion of bacterial cellulose ribbons reveals the endo character of the cellobiohydrolase Cel6A from Humicola insolens and its mode of synergy with cellobiohydrolase Cel7A. Appl. Environ. Microbiol. 2000, 66, 1444-52
29. Igarashi, K.; Uchihashi, T.; Koivula, A.; Wada, M.; Kimura, S.; Okamoto, T.; Penttila, M.; Ando, T.; Samejima, M. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science 2011, 333, 1279-82
30. Resch, M. G.; Donohoe, B. S.; Baker, J. O.; Decker, S. R.; Bayer, E. A.; Beckham, G. T.; Himmel, M. E. Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction. Energy Environ. Sci. 2013, 6, 1858-1867
31. Brunecky, R.; Alahuhta, M.; Xu, Q.; Donohoe, B. S.; Crowley, M. F.; Kataeva, I. A.; Yang, S. J.; Resch, M. G.; Adams, M. W.; Lunin, V. V.; et al. Revealing nature's cellulase diversity: The digestion mechanism of Caldicellulosiruptor bescii CelA. Science 2013, 342, 1513-6
32. Bubner, P.; Plank, H.; Nidetzky, B. Visualizing cellulase activity. Biotechnol. Bioeng. 2013, 110, 1529-49
33. Lamed, R.; Kenig, R.; Morag, E.; Calzada, J. F.; Demicheo, F.; Bayer, E. A. Efficient cellulose solubilization by a combined cellulosome-beta-glucosidase system. Appl. Biochem. Biotechnol. 1991, 27, 173-183
34. Jalak, J.; Väljamäe, P. Mechanism of initial rapid rate retardation in cellobiohydrolase catalyzed cellulose hydrolysis. Biotechnol. Bioeng. 2010, 106, 871-883
35. Cruys-Bagger, N.; Elmerdahl, J.; Praestgaard, E.; Tatsumi, H.; Spodsberg, N.; Borch, K.; Westh, P. Pre-steady-state kinetics for hydrolysis of insoluble cellulose by cellobiohydrolase Cel7A. J. Biol. Chem. 2012, 287, 18451-18458
36. Eggeman, T.; Elander, R. T. Process and economic analysis of pretreatment technologies. Bioresour. Technol. 2005, 96, 2019-2025
37. Kim, Y.; Mosier, N. S.; Ladisch, M. R.; Pallapolu, V. R.; Lee, Y. Y.; Garlock, R.; Balan, V.; Dale, B. E.; Donohoe, B. S.; Vinzant, T. B.; et al. Comparative study on enzymatic digestibility of switchgrass varieties and harvests processed by leading pretreatment technologies. Bioresour. Technol. 2011, 102, 11089-96
38. Wyman, C. E.; Dale, B. E.; Elander, R. T.; Holtzapple, M.; Ladisch, M. R.; Lee, Y. Y. Coordinated development of leading biomass pretreatment technologies. Bioresour. Technol. 2005, 96, 1959-1966
39. Wyman, C. E.; Dale, B. E.; Elander, R. T.; Holtzapple, M.; Ladisch, M. R.; Lee, Y. Y. Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover. Bioresour. Technol. 2005, 96, 2026-2032
40. Wyman, C. E.; Dale, B. E.; Elander, R. T.; Holtzapple, M.; Ladisch, M. R.; Lee, Y. Y.; Mitchinson, C.; Saddler, J. N. Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies. Biotechnol. Prog. 2009, 25, 333-339
41. Black, S. K.; Hames, B. R.; Myers, M. D. Method of Separating Lignocellulosic Material into Lignin, Cellulose and Dissolved Sugars, U.S Patent 5,730,837, 1998
42. Kurabi, A.; Berlin, A.; Gilkes, N.; Kilburn, D.; Bura, R.; Robinson, J.; Markov, A.; Skomarovsky, A.; Gusakov, A.; Okunev, O.; et al. Enzymatic hydrolysis of steam-exploded and ethanol organosolvpretreated Douglas-Firby novel and commercial fungal cellulases. Appl. Biochem. Biotechnol. 2005, 121-124, 219-30
43. Pan, X.; Xie, D.; Gilkes, N.; Gregg, D. J.; Saddler, J. N. Strategies to enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content. Appl. Biochem. Biotechnol. 2005, 121-124, 1069-79
44. Bozell, J. J.; Black, S. K.; Myers, M.; Cahill, D.; Miller, W. P.; Park, S. Solvent fractionation of renewable woody feedstocks: Organosolv generation of biorefinery process streams for the production of biobased chemicals. Biomass Bioenergy 2011, 35, 4197-4208
45. Bozell, J. J.; O'Lenick, C. J.; Warwick, S. Biomass fractionation for the biorefinery: Heteronuclear multiple quantum coherencenuclear magnetic resonance investigation of lignin isolated from solvent fractionation of switchgrass. J. Agric. Food Chem. 2011, 59, 9232-9242
46. Banerjee, G.; Car, S.; Liu, T. J.; Williams, D. L.; Meza, S. L.; Walton, J. D.; Hodge, D. B. Scale-up and integration of alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis, and ethanolic fermentation. Biotechnol. Bioeng. 2012, 109, 922-931
47. Banerjee, G.; Car, S.; Scott-Craig, J. S.; Hodge, D. B.; Walton, J. D., Alkaline peroxide pretreatment of corn stover: Effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose. Biotechnol. Biofuels 2011, 4
48. Katahira, R.; McKinney, K.; MIttal, A.; Ciesielski, P. N.; Donohoe, B. S.; Black, S. K.; Johnson, D. K.; Biddy, M. J.; Beckham, G. T. Evaluation of clean fractionation pretreatment for the production of renewable fuels and chemicals from corn stover. ACS Sustainable Chem. Eng. 2014, Doi: 10.1021/sc5001258
49. Chen, X. W.; Shekiro, J.; Franden, M. A.; Wang, W.; Zhang, M.; Kuhn, E.; Johnson, D. K.; Tucker, M. P. The impacts of deacetylation prior to dilute acid pretreatment on the bioethanol process. Biotechnol. Biofuels 2012, 5, 8
50. Li, X.; Ximenes, E.; Kim, Y.; Slininger, M.; Meilan, R.; Ladisch, M.; Chapple, C. Lignin monomer composition affects Arabidopsis cellwall degradability after liquid hot water pretreatment. Biotechnol. Biofuels 2010, 3, 27
51. Ximenes, E.; Kim, Y.; Mosier, N.; Dien, B.; Ladisch, M. Inhibition of cellulases by phenols. Enzyme Microb. Technol. 2010, 46, 170-176
52. Yang, B.; Wyman, C. E. BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. Biotechnol. Bioeng. 2006, 94, 611-617
53. Qing, Q.; Yang, B.; Wyman, C. E. Impact of surfactants on pretreatment of corn stover. Bioresour. Technol. 2010, 101, 5941-5951
54. Donohoe, B. S.; Selig, M. J.; Viamajala, S.; Vinzant, T. B.; Adney, W. S.; Himmel, M. E. Detecting cellulase penetration into corn stover cell walls by immuno-electron microscopy. Biotechnol. Bioeng. 2009, 103, 480-489
55. Donohoe, B. S.; Decker, S. R.; Tucker, M. P.; Himmel, M. E.; Vinzant, T. B. Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment. Biotechnol. Bioeng. 2008, 101, 913-925
56. Roche, C. M.; Dibble, C. J.; Stickel, J. J. Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings. Biotechnol. Biofuels 2009, 2, 28
57. Ciesielski, P. N.; Matthews, J. F.; Tucker, M. P.; Beckham, G. T.; Crowley, M. F.; Himmel, M. E.; Donohoe, B. S. 3D Electron tomography of pretreated biomass informs atomic modeling of cellulose microfibrils. ACS Nano 2013, 7, 8011-8019
58. Sluiter, J. B.; Ruiz, R. O.; Scarlata, C. J.; Sluiter, A. D.; Templeton, D. W. Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods. J. Agric. Food Chem. 2010, 58, 9043-9053.