Identification of Mechanism-Based Inactivation in P450-Catalyzed Cyclopropanation Facilitates Engineering of Improved Enzymes

Journal of the American Chemical Society

Volumn 138, Issue 38, 2016, Pages 12527-12533

  Renata, Hans ^a,c   Lewis, Russell D ^a   Sweredoski, Michael J ^b   Moradian, Annie ^b   Hess, Sonja ^b   Wang, Z Jane ^a   Arnold, Frances H ^a  

^a Division of Chemistry and Chemical Engineering   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; MASS SPECTROMETRY; PORPHYRINS;

CARBENE TRANSFER; COVALENT MODIFICATIONS; CYCLOPROPANATION; DIAZO COMPOUNDS; ENZYME FUNCTIONS; ETHYL DIAZOACETATE; INACTIVATION PATHWAY; PROTEOMIC ANALYSIS;

ENZYMES;

CARBENE; CYTOCHROME P450 BM3; DIAZOACETIC ACID ETHYL ESTER; HEMOPROTEIN; CYTOCHROME P450; CYTOCHROME P450 INHIBITOR; HEME;

AMINO ACID SEQUENCE; ARTICLE; BIOCATALYSIS; CONTROLLED STUDY; COVALENT BOND; CYCLOPROPANATION; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME INACTIVATION; ESCHERICHIA COLI; NONHUMAN; NUCLEOPHILICITY; PROTEIN MODIFICATION; SITE DIRECTED MUTAGENESIS; STEREOSELECTIVITY; STRUCTURE ANALYSIS; CATALYSIS; CHEMISTRY; CLASSIFICATION; COENZYME; METABOLISM; MOLECULAR MODEL; PROCEDURES; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

AMINO ACID SEQUENCE; CATALYSIS; COENZYMES; CYTOCHROME P-450 ENZYME INHIBITORS; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; HEME; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

EID: 84989221063     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/jacs.6b06823     Document Type: Article

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