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Volumn 20, Issue 13, 2010, Pages 3855-3858

Structural change of the enterobactin synthetase in crowded solution and its relation to crowding-enhanced product specificity in nonribosomal enterobactin biosynthesis

Author keywords

Enterobactin; Macromolecular crowding; Nonribosomal peptide synthetase

Indexed keywords

ENTEROBACTIN SYNTHETASE; ENTEROCHELIN; GLYCEROL; SYNTHETASE; UNCLASSIFIED DRUG;

EID: 77954316300     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.05.051     Document Type: Article
Times cited : (10)

References (20)
  • 9
    • 0035478585 scopus 로고    scopus 로고
    • R.J. Ellis TIBS 26 2001 597
    • (2001) TIBS , vol.26 , pp. 597
    • Ellis, R.J.1
  • 13
    • 77954314495 scopus 로고    scopus 로고
    • note
    • 2 and 5 mM DTT. The pH value of the mixture was re-adjusted after addition of the additives. Aliquots (100 μL each) were taken from a reaction mixture at an appropriate time interval and quenched immediately by addition of 15 μL of 1 N HCl. In investigation of the glycerol effect, enzyme concentrations were at their physiological concentrations (EntE 340 nM; holo-EntB 1.4 μM and holo-EntF 650 nM) and the reaction mixture was incubated at 37 °C for 1.5 h before quenching with HCl.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.