Enhanced protein and biochemical production using CRISPRi-based growth switches

Metabolic Engineering

Volumn 38, Issue , 2016, Pages 274-284

  Li, Songyuan ^a   Jendresen, Christian Bille ^a   Grünberger, Alexander ^b   Ronda, Carlotta ^a   Jensen, Sheila Ingemann ^a   Noack, Stephan ^b   Nielsen, Alex Toftgaard ^a  

^a Chalmers University of Technology   (Denmark)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

CRISPRi; Growth switch; Metabolic engineering; Mevalonate production; Protein production

Indexed keywords

CARBON; ESCHERICHIA COLI; MACHINERY; METABOLIC ENGINEERING;

CRISPRI; DNA REPLICATIONS; INCREASING PRODUCTION; MEVALONATE; MICRO FLUIDIC SYSTEM; MICROBIAL CELL FACTORIES; NUCLEOTIDE SYNTHESIS; PROTEIN PRODUCTION;

PROTEINS;

CRISPR ASSOCIATED PROTEIN; ISOPRENOID; MEVALONIC ACID; BACTERIAL PROTEIN; CARBON; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BIOCHEMISTRY; ESCHERICHIA COLI; METABOLIC ENGINEERING; NONHUMAN; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SINGLE CELL ANALYSIS; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; METABOLISM; PROCEDURES; REGULATOR GENE; SYNECHOCOCCUS; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; CARBON; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENES, SWITCH; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; RECOMBINANT PROTEINS; SYNECHOCOCCUS; TRANSCRIPTIONAL ACTIVATION;

EID: 84988911466     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2016.09.003     Document Type: Article

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