Surgical extraction of human dorsal root ganglia from organ donors and preparation of primary sensory neuron cultures

Nature Protocols

Volumn 11, Issue 10, 2016, Pages 1877-1888

  Valtcheva, Manouela V ^a,b   Copits, Bryan A ^a   Davidson, Steve ^a,c   Sheahan, Tayler D ^a,b   Pullen, Melanie Y ^a,b   McCall, Jordan G ^a   Dikranian, Krikor ^a   Gereau, Robert W ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY   (United States)

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM;

ARTICLE; CELL ASSAY; CELL ISOLATION; CELL STRUCTURE; CELL SUBPOPULATION; CLINICAL PROTOCOL; EXTRACTION; FLUORESCENCE ANALYSIS; HUMAN; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MOLECULAR IMAGING; NERVE CELL CULTURE; NERVOUS SYSTEM ELECTROPHYSIOLOGY; ORGAN DONOR; PRIORITY JOURNAL; PROCESS DEVELOPMENT; SENSORY NERVE CELL; SPINAL GANGLION; VIRAL GENE DELIVERY SYSTEM; CELL CULTURE; CELL CULTURE TECHNIQUE; CELL SEPARATION; CYTOLOGY; GENE TRANSFER; GENETIC TRANSDUCTION; GENETICS; METABOLISM; PROCEDURES; SURGERY; TRANSPLANTATION; VIRUS;

CALCIUM; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; GANGLIA, SPINAL; GENE TRANSFER TECHNIQUES; HUMANS; SENSORY RECEPTOR CELLS; TISSUE AND ORGAN PROCUREMENT; TRANSDUCTION, GENETIC; VIRUSES;

EID: 84988624574     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.111     Document Type: Article

References (43)

1. Lacroix-Fralish, M. L., Ledoux, J. B. & Mogil, J. S. The pain genes database: An interactive web browser of pain-related transgenic knockout studies. Pain 131, 3. e1-4 (2007).
2. Chizh, B. A. et al. The effects of the TRPV1 antagonist SB-705498 on TRPV1 receptor-mediated activity and inflammatory hyperalgesia in humans. Pain 132, 132-141 (2007).
3. Kivitz, A. J. et al. Efficacy and safety of tanezumab versus naproxen in the treatment of chronic low back pain. Pain 154, 1009-1021 (2013).
4. Skljarevski, V. et al. Efficacy of duloxetine in patients with chronic pain conditions. Current Drug Ther. 6, 296-303 (2011).
5. Wernicke, J. F. et al. A randomized controlled trial of duloxetine in diabetic peripheral neuropathic pain. Neurology 67, 1411-1420 (2006).
6. Hill, R. NK1 (substance P) receptor antagonists-why are they not analgesic in humans? Trends in Pharmacol. Sci. 21, 244-246 (2000).
7. Taneja, A., Di Iorio, V. L., Danhof, M. & Della Pasqua, O. Translation of drug effects from experimental models of neuropathic pain and analgesia to humans. Drug Discov. Today 17, 837-849 (2012).
8. Mogil, J. S. Animal models of pain: Progress and challenges. Nat. Rev. Neurosci. 10, 283-294 (2009).
9. Contopoulos-Ioannidis, D. G., Ntzani, E. & Ioannidis, J. P. Translation of highly promising basic science research into clinical applications. Am. J. Med. 114, 477-484 (2003).
10. Ergorul, C. & Levin, L. A. Solving the lost in translation problem: Improving the effectiveness of translational research. Curr. Opin. Pharmacol. 13, 108-114 (2013).
11. Hug, A. & Weidner, N. From bench to beside to cure spinal cord injury: lost in translation? Int. Rev. Neurobiol. 106, 173-196 (2012).
12. Gereau, R. W. et al. A pain research agenda for the 21st century. J. Pain 15, 1203-1214 (2014).
13. Han, L. et al. A subpopulation of nociceptors specifically linked to itch. Nat. Neurosci. 16, 174-182 (2012).
14. Liu, Q. et al. The distinct roles of two GPCRs, MrgprC11 and PAR2, in itch and hyperalgesia. Sci. Signal. 4, ra45 (2011).
15. Liu, Q. et al. Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus. Cell 139, 1353-1365 (2009).
16. Solinski, H. J., Gudermann, T. & Breit, A. Pharmacology and signaling of MAS-related G protein-coupled receptors. Pharmacol. Rev. 66, 570-597 (2014).
17. Solinski, H. J., Zierler, S., Gudermann, T. & Breit, A. Human sensory neuron-specific Mas-related G protein-coupled receptors-X1 sensitize and directly activate transient receptor potential cation channel V1 via distinct signaling pathways. J. Biol. Chem. 287, 40956-40971 (2012).
18. Zhang, X. L., Lee, K. Y., Priest, B. T., Belfer, I. & Gold, M. S. Inflammatory mediator-induced modulation of GABA currents in human sensory neurons. Neuroscience 310, 401-409 (2015).
19. Li, Y. et al. The cancer chemotherapeutic paclitaxel increases human and rodent sensory neuron responses to TRPV1 by activation of TLR4. J. Neurosci. 35, 13487-13500 (2015).
20. Anand, U. et al. Angiotensin II type 2 receptor (AT2 R) localization and antagonist-mediated inhibition of capsaicin responses and neurite outgrowth in human and rat sensory neurons. Eur. J. Pain 17, 1012-1026 (2013).
21. Davidson, S. et al. Human sensory neurons: membrane properties and sensitization by inflammatory mediators. Pain 155, 1861-1870 (2014).
22. Davidson, S. et al. Group II mGluRs suppress hyperexcitability in mouse and human nociceptors. Pain doi:10. 1097/j. pain. 0000000000000621, published online (21 May 2016).
23. Iyer, S. M. et al. Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nat. Biotechnol. 32, 274-278 (2014).
24. Park, S. I. et al. Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics. Nat. Biotechnol. 33, 1280-1286 (2015).
25. Copits, B. A., Pullen, M. Y. & Gereau, R. W. Spotlight on pain: Optogenetic approaches for interrogating somatosensory circuits. Pain doi:10. 1097/j. pain. 0000000000000620 (2016).
26. Yang, D. & Gereau, R. W. Peripheral group II metabotropic glutamate receptors (mGluR2/3) regulate prostaglandin E2-mediated sensitization of capsaicin responses and thermal nociception. J. Neurosci. 22, 6388-6393 (2002).
27. Hu, H. J., Bhave, G. & Gereau, R. W. Prostaglandin and protein kinase A-dependent modulation of vanilloid receptor function by metabotropic glutamate receptor 5: Potential mechanism for thermal hyperalgesia. J. Neurosci. 22, 7444-7452 (2002).
28. Jin, X. & Gereau, R. W. Acute p38-mediated modulation of tetrodotoxin-resistant sodium channels in mouse sensory neurons by tumor necrosis factor-alpha. J. Neurosci. 26, 246-255 (2006).
29. Yang, D. & Gereau, R. W. Group II metabotropic glutamate receptors inhibit cAMP-dependent protein kinase-mediated enhancemednt of tetrodotoxin-resistant sodium currents in mouse dorsal root ganglion neurons. Neurosci. Lett. 357, 159-162 (2004).
30. Valtcheva, M. V., Davidson, S., Zhao, C., Leitges, M. & Gereau, R. W. Protein kinase Cdelta mediates histamine-evoked itch and responses in pruriceptors. Mol. Pain 11, 1 (2015).
31. Valtcheva, M. V., Samineni, V. K., Golden, J. P., Gereau, R. W. & Davidson, S. Enhanced nonpeptidergic intraepidermal fiber density and an expanded subset of chloroquine-responsive trigeminal neurons in a mouse model of dry skin itch. J. Pain 16, 346-356 (2015).
32. Sheahan, T. D., Copits, B. A., Golden, J. P. & Gereau, R. W. Voluntary exercise training: Analysis of mice in uninjured, inflammatory, and nerve-injured pain states. PLoS One 10, e0133191 (2015).
33. Blanchard, J. W. et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat. Neurosci. 18, 25-35 (2015).
34. Wainger, B. J. et al. Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts. Nat. Neurosci. 18, 17-24 (2015).
35. Zhao, S. et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nat. Methods 8, 745-752 (2011).
36. Ting, J. T., Daigle, T. L., Chen, Q. & Feng, G. Acute brain slice methods for adult and aging animals: Application of targeted patch clamp analysis and optogenetics. Methods Mol. Biol. 1183, 221-242 (2014).
37. Hille, B. The permeability of the sodium channel to organic cations in myelinated nerve. J. Gen. Physiol. 58, 599-619 (1971).
38. Siuda, E. R. et al. Spatiotemporal control of opioid signaling and behavior. Neuron 86, 923-935 (2015).
39. Goverdhana, S. et al. Regulatable gene expression systems for gene therapy applications: Progress and future challenges. Mol. Ther. 12, 189-211 (2005).
40. Glorioso, J. C. & Fink, D. J. Herpes vector-mediated gene transfer in the treatment of chronic pain. Mol. Ther. 17, 13-18 (2009).
41. Hsu, P. D., Lander, E. S. & Zhang, F. Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262-1278 (2014).
42. Incontro, S., Asensio, C. S., Edwards, R. H. & Nicoll, R. A. Efficient, complete deletion of synaptic proteins using CRISPR. Neuron 83, 1051-1057 (2014).
43. Bhave, G. et al. Protein kinase C phosphorylation sensitizes but does not activate the capsaicin receptor transient receptor potential vanilloid 1 (TRPV1). Proc. Natl. Acad. Sci. USA 100, 12480-12485 (2003).