A counterselection method for Lactococcus lactis genome editing based on class IIa bacteriocin sensitivity

Applied Microbiology and Biotechnology

Volumn 100, Issue 22, 2016, Pages 9661-9669

  Wan, Xing ^a   Usvalampi, Anne M ^a,b   Saris, Per E J ^a   Takala, Timo M ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b AALTO UNIVERSITY   (Finland)

Author keywords

Class IIa bacteriocin; Counterselection; Genome editing; Lactic acid bacteria

Indexed keywords

ANTIBIOTICS; CHROMOSOMES; DNA; ESCHERICHIA COLI; GENE ENCODING; GENES; INTEGRATION; LACTIC ACID; POLYPEPTIDES; VECTORS;

BACTERIOCIN RESISTANCE; BACTERIOCINS; COUNTERSELECTION; ERYTHROMYCIN RESISTANCE; HOMOLOGOUS RECOMBINATION; LACTIC ACID BACTERIA; LISTERIA MONOCYTOGENES; PHOSPHOTRANSFERASE SYSTEM;

C (PROGRAMMING LANGUAGE);

BACTERIOCIN; CLASS IIA BACTERIOCIN; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT;

AGAR; ANTIBIOTIC RESISTANCE; ANTIBIOTICS; BACTERIUM; CHROMOSOME; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; MUTATION; PLASMID;

ARTICLE; CHROMOSOME DELETION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE; GENOME ANALYSIS; GENOME EDITING; LACTOCOCCUS LACTIS; LISTERIA MONOCYTOGENES; MPTC GENE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; DRUG EFFECTS; GENE EDITING; GENE VECTOR; GENETIC SELECTION; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; MICROBIAL VIABILITY; PROCEDURES;

ESCHERICHIA COLI; LACTOCOCCUS LACTIS; LISTERIA MONOCYTOGENES;

ANTI-BACTERIAL AGENTS; BACTERIOCINS; GENE EDITING; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; LACTOCOCCUS LACTIS; MICROBIAL VIABILITY; PLASMIDS; SELECTION, GENETIC;

EID: 84988600663     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-016-7828-6     Document Type: Article

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