Antarctic Lakes as Models for the Study of Microbial Biodiversity, Biogeography and Evolution

Antarctic Ecosystems: An Extreme Environment in a Changing World

Volumn , Issue , 2011, Pages 63-89

  Pearce, David A ^a   Laybourn Parry, Johanna ^b  

^a BRITISH ANTARCTIC SURVEY   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Antarctic lake types; Antarctic lakes, models; Biogeography, spatial variation; Methods, exploring Antarctic biodiversity; Microbial biodiversity, biogeography; Microbial groups; Palaeolimnology; Physical, chemical lake environment; Prokaryote, eukaryote physiology

Indexed keywords

EID: 84985867963     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781444347241.ch3     Document Type: Chapter

References (267)

1. Adamson, D. & Darragh, A. 1991 Field evidence on Cainozoic history and landforms in the northern Prince Charles Mountains, East Antarctica. In Quarternary research in Australian Antarctica: future directions, vol. 3 (eds. D. Gillieson & S. Fitzsimmons) Special publication, pp. 5-14. Canberra, Australia: Department of Geography and Oceanography, University of New South Wales/AustralianDefence Force Academy.
2. Adamson, D.A. & Pickard, J. 1986 Cainozoic history of the Vestfold Hills. In: Antarctic oasis. Terrestrial environment and history of the Vest fold Hüls (ed.J. Pickard), pp. 63-97. Sydney, Australia; New York, NY; London, UK: Academic Press.
3. Akatova, N.A. 1966 On the occurrence of Daphniopsis studeri Ruhe (Cladocera) in Lake Posadochnoe of the Vestfold "Oasis" (Eastern Antarctica). In: Studies of marine fauna. Biological reports of the Soviet Antarctic expedition, 1955-1958, vol 2 (eds. A. P. Andriyasev & P. K. Ushaleov), pp. 190-193. Jersusalem, Israel: Israel Program for Scientific Translation. [Transl. from Issled Fauney Morei 2 1964.
4. Anesio, A.M., Mindl, B., Laybourn-Parry, J., Hodson, A.J. and Sattler, B. (2007) Viral dynamics in cryoconite holes on a high Arctic glacier (Svalbard), Journal of Geophysical Research (Biogeosciences), 112, G04S31. doi: 10.1029/2006JG000350
5. Alam, S.I., Dixit, A., Reddy, G.S.N., Dube, S., Palit, M., Shivaji, S. and Singh, L. 2006 Clostridium schirmacherense. sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica. Int J Syst Evol Microbiol, 56, 715.
6. Allen, M., Lauro, F.M., Williams, T.J., Burg, D., Siddiqui, K.S., De Francisci, D., Chong, K.W.Y., Pilak, O., Chew, H.H., De Maere, M.Z., Ting, L., Katrib, M., Ng, C., Sowers, K.R., Galperin, M.Y., Anderson, I.J., Ivanova, N., Dalin, E., Martinez, M., Lapidus, A., Hauser, L., Land, M., Thomas, T. & Cavicchioli, R. 2009 The genome sequence of the psychrophilic archaeon, Methanococcoides burtonii: the role of genome evolution in cold-adaptation. International Society of Microbial Ecology Journal, 3, 1012.
7. Baas-Becking, L.G.M. 1934 Geobiologie of inleiding tot de milieukunde. The Hague, The Netherlands: Van Stockum & Zon.
8. Baker, G.C., Tow, L.A. & Cowan, D.A. 2003 PCR based detection of non-indigenous microorganisms in 'pristine' environments. J. Microbiol. Methods, 53, 157-164. doi: 10.1016/S0167-7012(03)00021-6
9. Bayliss, P.R. & Laybourn-Parry, J. 1995 Seasonal abundance and size variation in Antarctic populations of the cladoceran Daphniopsis studeri. Antarct. Sci., 7, 393.
10. Bayliss, P., Ellis-Evans, J.C. & Laybourn-Parry, J. 1997 Temporal patterns of primary production in a large ultraoligotrophic Antarctic freshwater lake. Polar Biol., 18, 363-370. doi: 10.1007/s003000050201
11. Bayly, I.A.E. 1986 Ecology of the zooplankton of a meromictic Antarctic lagoon with special reference to Drepanopus bispinosus (Copepoda: Calanoida). Hydrobiologia, 140, 199-231. doi: 10.1007/BF00007436
12. Bayly, I.A.E. & Burton, H.R. 1993 Beaver Lake, greater Antarctica, and its population of Boeckella poppei (Mrázek) (Copepoda: Calanoida). Int. Veren. Theor. Ang. Limnol., 25, 975.
13. Bayly, I.A.E., Gibson, J.A.E., Wagner, B. & Swadling, K.M. 2003 Taxonomy, ecology and zoogeography of two East Antarctic freshwater calanoid copepod species: Boeckella poppei and Gladioferens antarcticus. Antarct. Sci., 15, 439-448. doi: 10.1017/S0954102003001548
14. Beijerinck, M.W. 1913 De infusies en de ontdekking der bakteriën. Jaarboeck van de Koninlijke Akademie v. Wetenschappen. Amsterdam, The Netherlands: Müller.
15. Bell, E.M. & Laybourn-Parry, J. 1999a Annual plankton dynamics in an Antarctic saline lake. Freshw. Biol., 41, 507-519. doi: 10.1046/j.1365-2427.1999.00396.x
16. Bell, E.M. & Laybourn-Parry, J. 1999b The plankton community of a young, eutrophic Antarctic saline lake. Polar Biol., 22, 248-253. doi: 10.1007/s003000050417
17. Bell, E.M. & Laybourn-Parry, J. 2003 Mixotrophy in the Antarctic phytoflagellate Pyramimonas gelidocola McFadden (Chlorophyta: Prasinophyceae). J. Phycol., 39, 644-649. doi: 10.1046/j.1529-8817.2003.02152.x
18. Bharathi, P.A.L., Nair, S., De Souza, M.J. & Chandramohan, D. 1999 Truce with oxygen-anaerobiosis outcompetes aero-biosis in the Antarctic lacustrine bacteria. Curr. Sci., 76, 1585.
19. Bowman, J.P. 2004 Psychrophilic prokaryote structural functional relationships, biogeography and evolution within marine sediment. Cell. Mol. Biol., 50, 503.
20. Bowman, J.P., Rea, S.M., McCammon, S.A. & McMeekin, T.A. 2000 Diversity and community structure within anoxic sediment from marine salinity meromictic lakes and a coastal meromictic marine basin, Vestfold Hills, eastern Antarctica. Environ. Microbiol., 2, 227-237. doi: 10.1046/j.1462-2920.2000.00097.x
21. Bowman, J.P., Mancuso, C., Nichols, C.M. & Gibson, J.A.E. 2003 Algoriphagus ratowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., Cryomorpha ignava gen. sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int. J. Syst. Evol. Microbiol. 53, 1343-1355. doi: 10.1099/ijs.0.02553-0
22. Bowman, J.P., McCammon, S.A., Rea, S.M. & McMeekin, T.A. 2006 The microbial composition of three limnologically disparate hypersaline Antarctic lakes. FEMS Microbiology Letters, 183, 81.
23. Bratina, B.J., Stevenson, B.S., Green, W.J. & Schmidt, T.M. 1998 Manganese reduction by microbes from oxic regions of the Lake Vanda (Antarctica) water column. Appl. Environ. Microbiol., 64, 3791.
24. Bulat, S.A. et al. 2004 DNA signature of thermophilic bacteria from the aged accretion ice of Lake Vostok, Antarctica: implications for searching for life in extreme icy environments. Int. J. Astrobiol., 3, 1-12. doi: 10.1017/S1473550404001879
25. Burgess, J.S., Spate, A.P. & Shevelin, J. 1994 The onset of glaciation in the Larsemann Hills, eastern Antarctica. Antarct. Sci., 6, 491.
26. Burke, C.M. & Burton, H.R. 1988 Photosynthetic bacteria in meromictic lakes and stratified fjords of the Vestfold Hills. Hydrobiologia, 165, 12.
27. Burton, H.J. 1981 Chemistry, physics and evolution of Antarc-tic saline lakes a review. Hydrobiologica, 82, 339-362. doi: 10.1007/BF00048724
28. Busse, H.J., Denner, E.B.M., Buczolits, S., Salkinoja-Salonen, M., Bennasar, A. & Kampfer, P. 2003 Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustbourne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas. Int. J. Syst. Evol. Microbiol., 53, 1253-1260. doi: 10.1099/ijs.0.02461-0
29. Cavicchioli, R. 2006 Cold adapted Archaea. Nature Rev Microbio., 14, 331.
30. Cerny, M. & Hebert, P.D.N. 1999 Intercontinental allozyme differentiation among four holarctic Daphnia species. Lim-nol. Oceanogr., 44, 1381.
31. Chan, O.C., Wolf, M., Hepperle, D. & Casper, P. 2002 Metha-nogenic archaeal community in the sediment of an artificially partitioned acidic bog lake. FEMS Microbiol. Ecol., 42, 119-129. doi: 10.1111/j.1574-6941.2002.tb01001.x
32. Chantila, K., Demers, S., Mostajir, B., Gosselin, M., Chanut, J. P., Monfort, P. & Bird, D. 2001 The responses of a natural bacterioplankton community to different levels of ultravio-let-B radiation: a food web perspective. Microb. Ecol., 41, 56.
33. Chattopadhyay, M.K. 2000 Cold adaptation of Antarctic mi-croorganisms-possible involvement of viable but non cul-turable state. Polar Biol., 23, 223-224. doi: 10.1007/s003000050030
34. Chattopadhyay, M.K., Uma Devi, K. & Shivaji, S. 1995 Thermostable alkaline phosphatase from Sphingobacterium ant-arcticus, a psychrotrophic bacterium from Antarctica. Polar Biol., 15, 215-219. doi: 10.1007/BF00239061
35. Chattopadhyay, M.K., Jagannadham, M.V., Vairamani, M. & Shivaji, S. 1997 Carotenoid pigments of an Antarctic psy-chrotrophic bacterium Micrococcus roseus: temperature dependent biosynthesis, structure and interaction with synthetic membranes. Biochem. Biophys. Res. Com., 239, 85-90. doi: 10.1006/bbrc.1997.7433
36. Chen, M., Xiao, X., Wang, P., Zeng, X. & Wang, F.P. 2005 Arthrobacter ardleyensis sp. nov., isolated from Antarctic lake sediment and deep-sea sediment. Arch. Microbiol., 183, 301-305. doi: 10.1007/s00203-005-0772-y
37. Chong, C.W., Pearce, D.A., Convey, P., TanG.Y.A., Wong, R.C. S & Tan, I. K. P. 2010 High levels of spatial heterogeneity in the biodiversity of soil prokaryotes on Signy Island, Antarctica. Soil Biology and Biogeochemistry, 42, 601.
38. Christner, B.C., Mosley-Thompson, E., Thompson, L.G. & Reeve, J.N. 2001 Isolation of bacteria and 16S rDNAs from Lake Vostoc accretion ice. Environ. Microbiol., 3, 570-577. doi: 10.1046/j.1462-2920.2001.00226.x
39. Christner, B.C., Kvitko, B.H. & Reeve, J.N. 2003 Molecular identification of bacteria and eukarya inhabiting an Antarctic cryoconite hole. Extremophiles, 7, 177.
40. Clark, M.S. et al. 2004 Antarctic genomes. Comp. Funct. Genomics, 5, 230-238. doi: 10.1002/cfg.398
41. Clocksin, K.M., Jung, D.O. & Madigan, M.T. 2007 Cold-active chemoorganotrophic bacteria from permanently ice-covered Lake Hoare, McMurdo Dry Valleys, Antarctica. Appl. Environ. Microbiol., 73, 3077.
42. Coker, J.A., Sheridan, P.P., Loveland-Curtze, J., Gutshall, K. R., Auman, A.J. & Brenchley, J. E. 2003 Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an Antarctic Arthrobac-ter isolate. J. Bacteriol., 185, 5473-5482. doi: 10.1128/JB.185.18.5473-5482.2003
43. Connelly, T.L., Tilburg, C.M. & Yager, P.L. 2006 Evidence for psychrophiles outnumbering psychrotolerant marine bacteria in the springtime coastal Arctic. Limnol. Oceanogr., 51, 1205.
44. Coolen, M.J.L., Muyzer, G., Rijpstra, W.I.C., Scouten, S., Volk-man, J.K. & Damste, J.S.S. 2004 Combined DNA and lipid analysis of sediments reveal changes in Holocene haptophyte and diatom populations in an Antarctic lake. Earth Planet. Sci. Lett., 223, 225-239. doi: 10.1016/j.epsl.2004.04.014
45. Cottrell, M.T., Yu, L.Y. & Kirchman, D.L. 2005 Sequence and expression analyses of Cytophaga-like hydrolases in a Western arctic metagenomic library and the Sargasso sea. Appl. Environ. Microbiol., 71, 8506-8513. doi: 10.1128/AEM.71.12.8506-8513.2005
46. Cowan, D., Meyer, Q., Stafford, W., Muyanga, S., Cameron, R. & Wittwer, P. 2005 Metagenomic gene discovery: past, present and future. Trends Biotechnol., 23, 321-329. doi: 10.1016/j.tibtech.2005.04.001-
47. Cowan, D.A. 2009 Cryptic microbial communities in Antarctic deserts. PNAS, 106, 19749.
48. Crawford, D.W. 1989 Mesodinium rubrum.: the phytoplankter that wasn't. Mar. Ecol. Prog. Ser., 58, 161.
49. Cromer, L., Gibson, J.A.E., Swadling, K.M. & Ritz, D.A. 2005 Faunal microfossils: indicators of Holocene ecological change in a saline Antarctic lake. Palaeogeogr. Palaeocli-matol. Palaeoecol., 221, 83-97. doi: 10.1016/j.palaeo.2005.02.005
50. Delille, D. 1996 Biodiversity and function of bacteria in the Southern Ocean. Biodivers. Conserv. 5, 1505-1523. doi: 10.1007/BF00051989
51. D'Elia, T., Veerapaneni, R. & Rogers, S.O. 2008 Isolation of microbes from Lake Vostok accretion ice. Appl. & Environ. Microbiol., 74, 4962.
52. Deming, J.W. 2002 Psychrophiles and polar regions. Curr. Opin. Microbiol., 5, 301-309. doi: 10.1016/S1369-5274(02)00329-6
53. De Wever, A., Leliaert, F., Verleyen E., Vanormelingen, P., Van der Gucht, K., Hodgson, D. A., Sabbe, K. & Vyverman, W. 2009 Hidden levels of phylodiversity in Antarctic green algae: further evidence of glacial refugia. Proc. R. Soc. B., 276, 3591.
54. Dhaked, R.K., Alam, S.I., Dixit, A. & Singh, L. 2005 Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp. P9 Enzyme Microb. Technol., 36, 855-861. doi: 10.1016/j.enzmictec.2004.11.017
55. Dobson, S.J., Colwell, R.R., McMeekin, T.A. & Franzmann, P.D. 1993 Direct sequencing of the polymerase chain reaction-amplified 16S ribosomal RNA gene of Flavobacterium salegens sp. nov. 2 new species from a hypersaline Antarctic lake. Int. J. Syst. Bacteriol., 43, 77.
56. Donachie, S.P., Bowman, J.P. & Alam, M. 2004 Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga-Flavobacterium-Bacteroides group bacterium from an Hawaiian hypersaline lake. Int. J. Syst. Evol. Microbiol., 54, 935-940. doi: 10.1099/ijs.0.02733-0
57. Doran, P.T., Wharton, J.R., Lyons, W.B., Des Marais, D.J. & Anderson, D.T. 2000 Sedimentology and geochemistry of a perennially ice-covered epishelf lake in the Bunger Hills Oasis, East Antarctica. Antarct. Sci., 12, 131.
58. Doran, P.T., Fritsen, C.H., McKay, C.P., Priscu, J.C. & Adams, E. E. 2003 Formation and character of an ancient 19-m ice cover and underlying trapped brine in an 'ice- sealed' East Antarctic lake. Proc. Natl Acad. Sci. USA, 100, 26-31. doi: 10.1073/pnas.222680999
59. Edmondson, W.T. 1955 The seasonal life history of Daphnia in an Arctic lake. Ecology, 36, 439-455. doi: 10.2307/1929580
60. Ellis-Evans, J.C., Laybourn-Parry, J., Bayliss, P. & Perriss, S.J. 1998 Microbial community structure in lakes of the Larse-mann Hills, continental Antarctica. Arch. Hydrobiol., 141, 209.
61. Ericsson, D.J., Kasrayan, A., Johansson I.P., Bergfors, T., Sandström, A.G., Bäckvall, J-E. & Mowbray, S.L. 2008 X-ray structure of Candida antarctica Lipase A shows a novel lid structure and a likely mode of interfacial activation. Journal of Molecular Biology, 376: 109-119.
62. Ewart, K.V., Yang, D.S., Ananthanarayanan, V.S., Fletcher, G. L. & Hew, C.L. 1996 Ca_sup_2C_/sup_-dependent antifreeze proteins. Modulation of conformation and activity by divalent metal ions. J. Biol. Chem., 271, 16,627-16,632. doi: 10.1074/jbc.271.8.4106
63. Fenchel, T. & Finlay, B.J. 2003 Is microbial diversity fundamentally different from biodiversity of larger animals and plants? Eur. J. Protistol., 39, 486-490. doi: 10.1078/09324739-00025
64. Fernandez-Valiente, E., Quesada, A., Howard-Williams, C. & Hawes, I. 2001 N2 fixation in cyanobacterial mats from ponds on the McMurdo Ice Shelf. Antarct. Microb. Ecol., 42, 338-349. doi: 10.1007/s00248-001-1010-z]
65. Ferrer, M., Golyshina, O., Beloqui, A. & Golyshin, P.N. 2007 Mining enzymes from extreme environments. Current Opinion in Microbiology, 10, 207.
66. Ferris, J.M. & Burton, H.R. 1988 The annual cycle of heat and mechanical stability of hypersaline Deep Lake, Vestfold Hills, Antarctica. Hydrobiologia, 165, 115-128. [doi: 10.1007/BF00025579]
67. Filina I.Y., Blankenship D.D., Thoma M., Lukin V.V., Masolov V.N., Sen M.K. 2008 New 3D bathymetry and sediment distribution in Lake Vostok: Implication for pre-glacial origin and numerical modelling of the internal processes within the lake. Earth and Planetary Science Letters, 276, 106.
68. Foreman, C.M., Sattler, B., Mikucki, J.A., Porazinska, D.L., & Priscu, J.C. 2007 Metabolic activity and diversity of cryo-conites in the Taylor Valley, Antarctica. J. Geophysical Research, 112, G04 S32, doi: 10.1029/2006JG000358.
69. Franzmann, P.D. & Dobson, S.J. 1993 The phylogeny of bacteria from a modern Antarctic refuge. Antarct. Sci., 5, 267.
70. Fujii, T., Oikawa, T., Muraoka, I., Soda, K. & Hata, Y. 2007 Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1. Acta Cryst., 63, 983.
71. Garneau, M.E., Vincent, W. F., Alonso-Saez, L., Gratton, Y. & Lovejoy, C. 2006 Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem. Aquat. Microb. Ecol., 42, 27.
72. Gasol, J.M., Guerrero, R. & Pedros-Alio, C. 1992 Spatial and temporal dynamics of a metalimnetic cryptomonas peak. J. Plankton Res., 14, 1565-1579. doi: 10.1093/plankt/14.11.1565
73. Gasol, J.M., Garciacantizano, J., Massana, R., Guerrero, R. & Pedros-Alio, C. 1993 Physiological ecology of a metalim-netic cryptomonas population-relationships to light, sulfide and nutrients. J. Plankton Res., 15, 255-275. doi: 10.1093/plankt/15.3.255
74. Gervais, F. 1998 Ecology of cryptophytes coexisting near a freshwater chemocline. Freshw. Biol., 39, 61-78. doi: 10.1046/j.1365-2427.1998.00260.x
75. Gibson, J.A.E. & Bayly, I.A.E. 2007 New insights into the origins of crustaceans of Antarctic lakes. Antarctic Science, 19, 157.
76. Gibson, J.A., Dartnall, H.J.G. & Swadling, K.M. 1998 On the occurrence of males and production of ephippial eggs in populations of Daphniopsis studeri (Cladocera) in lakes of the Vestfold and Larsemann Hills, East Antarctica. Polar Biol., 19, 148-150. doi: 10.1007/s003000050227
77. Gilbert, J.A., Hill, P.J., Dodd, C.E.R. & Laybourn-Parry, J. 2004 Demonstration of antifreeze protein activity in Antarctic lake bacteria. Microbiology, 150, 171-180. (doi: 10.1099/mic.0.26610-0)
78. Gilbert, J.A., Davies, P.L. & Laybourn-Parry, J. 2005 A hyperactive. Ca_sup_2C_/sup_ dependent antifreeze protein in an Antarctic bacterium. FEMS Microbiol. Lett., 245, 67-72. doi: 10.1016/j.femsle.2005.02.022
79. Glöckner, F.O., Zaichikov, E., Belkova, N., Denissova, L., Pernthaler, J., Pernthaler, A. & Amann, R. 2000 Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of Actinobacteria. Appl. Environ. Microbiol., 66, 5053-5065. doi: 10.1128/AEM.66.11.5053-5065.2000
80. Goodchild, A., Saunders, N.F.W., Ertan, H., Raftery, M., Guil-haus, M., Curmi, P.M.G. & Cavicchioli, R. 2004 A proteomic determination of cold adaptation in the Antarctic archaeon Methanococcoides burtonii. Mol. Microbiol., 53, 309-321. doi: 10.1111/j.1365-2958.2004.04130.x
81. Gordon, D.A., Priscu, J. & Giovannoni, S. 2000 Origin and phylogeny of microbes living in permanent lake ice. Microb. Ecol., 39, 197.
82. Griffith, M. & Yaish, M.W. 2004 Antifreeze proteins in overwintering plants: a tale of two activities. Trends Plant Sci. 9, 399-405. doi: 10.1016/j.tplants.2004.06.007
83. Grzymski, J.J., Carter, B.J., DeLong, E.F., Feldman, R.A., Gha-diri, A. & Murray, A.E. 2006 Comparative genomics of DNA fragments from six Antarctic marine planktonic bacteria. Appl. Environ. Microbiol., 72, 1532.
84. Gustafson, D.E., Stoeker, D.K., Johnson, M.D., Van Heukelem, W.F. & Sneider, K. 2000 Cryptophyte algae robbed of their organelles by the marine ciliate Mesodinium rubrum. Nature, 405, 1049-1052. doi: 10.1038/35016570
85. Hairston Jr, N.G. 1979 The adaptive significance of color polymorphism in two species of Diaptomus Copepoda. Lim-nol. Oceanogr., 24, 15.
86. Hall, B.L. & Henderson, G.M. 2001 Use of uranium- thorium dating to determine past C-14 reservoir effects in lakes: examples from Antarctica. Earth Planet. Sci. Lett., 193, 565-577. doi: 10.1016/S0012-821X(01)00524-6
87. Handelsman, J. 2004 Metagenomics: application of genomics to uncultured microorganisms. Microbiol. Mol. Biol. Rev., 68, 669-685. doi: 10.1128/MMBR.68.4.669-685.2004
88. Hann, B.J. 1986 Revision of the genus Daphniopsis SARS, 1903 (Cladocera, Daphniidae) and a description of Daph-niopsis chilensis, new species, from South America. J. Crust. Biol., 6, 246-263. doi: 10.2307/1547985
89. Hansson, L.-A. 2000 Induced pigmentation in zooplankton: a trade-off between threats from predation and ultraviolet radiation. Proc. R. Soc., B 267, 2327-2331. doi: 10.1098/rspb.2000.1287
90. Hansson, L.-A. 2004 Plasticity in pigmentation induced by conflicting threats from predation and UV radiation. Ecology, 85, 1005.
91. Hawes, I. & Schwartz, A.M. 1999 Photosynthesis in an extreme shade environment: benthic microbial mats from Lake Hoare, a permanently ice-covered Antarctic lake. J. Phycol., 35, 448-459. doi: 10.1046/j.1529-8817.1999.3530448.x
92. Hebert, P.D.N. 1981 Obligate asexuality inDaphnia. Am. Nat., 117, 784-789. doi: 10.1086/283761
93. Hendy, C.W., Wilson, A.T., Popplewell, K.B. & House, D.A. 1977 Dating of geochemical events in Lake Bonney, Antarctica, and their relationship to glacial and climate changes. NZ J. Geol. Geophys., 20, 1103.
94. Heywood, R.B. 1972 Antarctic limnology: a review. Br. Ant-arct. Surv. Bull., 29, 35.
95. Heywood, R.B. 1977 Antarctic freshwater ecosystems; review and synthesis. In Adaptations within Antarctic ecosystems. Proc. 3rd SCAR Symp. Antarct Biol. (ed. G. A. Llano), pp. 801-827. Washington, DC: Smithsonian Institute.
96. Hirsch, P., Gallikowski, C.A., Siebert, J., Peissl, K., Kroppenstedt, R., Schumann, P., Stakebrandt, E. & Anderson, R. 2004a Deionococcus frigens sp. nov., Deionococcus saxícola sp. nov., and Deionococcus marmoris sp. nov., low temperature and drought-tolerating, UV resistant bacteria from continental Antarctica. Syst. Appl. Microbiol., 27, 636-645. doi: 10.1078/0723202042370008
97. Hirsch, P., Mevs, U., Kroppenstedt, R.M., Schumann, P. & Stakebrandt, E. 2004b Cryptoendolithic actinomycetes from Antarctic sandstone rock samples: Micromonospora endo- lithica sp. nov. and two isolates related to Micro- monospora coerulea Jensen 1932. Syst. Appl. Microbiol., 27, 166-174. doi: 10.1078/072320204322881781
98. Hodgson, D.A., Noon, P.E., Vyverman, W., Bryant, C.L., Gore, D. B., Appleby, P., Gilmour, M., Verleyen, E., Sabbe, K., Jones, V. J., Ellis-Evans, J.C. & Wood, P.B. 2001 Were the Larsemann Hills ice-free through the last glacial maximum? Antarct. Sci., 13, 440-454. doi: 10.1017/S0954102001000608
99. Hodgson, D.A., Doran, P.T., Roberts, D. & McMinn, A. 2004 Palaeolimnological studies from the Antarctic and sub-Antarctic islands. In Long-term environmental change in Arctic and Antarctic lakes (eds. R. Pienitz, M.S.V. Douglas & J.P. Smol), pp. 419-474. The Hague, The Netherlands: Springer.
100. Hodgson, D.A., Roberts, S.J., Bentley, M. J, Smith, J.A., Johnson, J., Verleyen, E., Vyverman, W., Hodgson, A., Leng, M.J., Cziferszky, A., Fox, A. J. & Sanderson, D.C.W. 2009a Exploring former subglacial Hodgson Lake, Antarctica. Paper I: site description, geomorphology and limnology. Quaternary Science Reviews, 28, 2295.
101. Hodgson, D.A., Roberts, S.J., Bentley, M.J., Carmichael, E.L., Smith, J.A., Verleyen, E., Vyverman, W., Geissler, P., Leng, M.J. & Sanderson, D.C.W. 2009b Exploring former subglacial Hodgson Lake, Antarctica. Paper II: palaeolimnology. Quaternary Science Reviews, 28, 2310-2325.
102. Howard-Williams, C., Schwarz, A.-M., Hawes, I. & Priscu, J. C. 1998 Optical properties of the McMurdo Dry Valley lakes, Antarctica. In Ecosystem dynamics in a polar desert, the McMurdo dry valleys, Antarctica, vol 72 (ed. J. C. Priscu). Antarctic research series, pp. 189-204. Washington, DC: American Geophysical Union.
103. Ideno, A., Yoshida, T., Iida, T., Furutani, M. & Maruyama, T. 2001 FK506-binding protein of the hyperthermophilic ar-chaeum, Thermococcus sp. KS-1, a cold-shock inducible peptidyl-prolyl cis-trans isomerase with activities to trap and refold denatured proteins. Biochem. J., 357, 465-471. doi: 10.1042/0264-6021:3570465
104. James, S.R., Burton, H.R., McMeekin, T.A. & Mancuso, C.A. 1994 Seasonal abundance of Halomonas meridiana, Halomonas subglaciescola, Flavobacterium gondwanense and Flavobacterium salegens in 4 Antarctic lakes. Antarct. Sci., 6, 325.
105. Jones, V.J., Hodgson, D.A. & Chepstow-Lusty, A. 2000 Palaeo-limnological evidence for marked Holocene environmental changes on Signy Island, Antarctica. Holocene, 10, 43-60. doi: 10.1191/095968300673046662
106. Jung, D.O., Achenbach, L.A., Karr, E.A., Takaichi, S. & Madigan, M.T. 2004 A gas vesiculate planktonic strain of the purple non-sulfur bacterium Rhodoferax antarcticus isolated from Lake Fryxell, Dry Valleys, Antarctica. Arch. Microbiol., 182, 236-243. doi: 10.1007/s00203-004-0719-8
107. Jungblut, A., Hawes, I., Mountfort, D., Hitzfield, B., Dietrich, D. R., Burns, B.P. & Eilan, B.A. 2005 Diversity within cyano-bacterial mat communities in variable salinity meltwater ponds of McMurdo Ice Shelf, Antarctica. Environ. Microbiol., 7, 519-526. doi: 10.1111/j.1462-2920.2005.00717.x
108. Jungblut, A.D., Allena, A.A., Burnsa, B.P. and Neilan, B.A. 2009 Lipid biomarker analysis of cyanobacteria-dominated microbial mats in meltwater ponds on the McMurdo Ice Shelf, Antarctica. Organic Geochemistry, 40, 258.
109. Jungblut A.D., Lovejoy, C. & Vincent, W.F. 2010 Global distribution of cyanobacterial ecotypes in the cold biosphere. The ISME Journal, 4, 191.
110. Kan, J., Wang, K. & Chen, F. 2004 Are 5000 bacterial cells per ml_sup_-1_/sup_ the detection threshold for PCR-DGGE analysis? In: Int. Symp. on Microbial Ecology-10 (ISME 10), Cancun, Mexico, 22-27 August 2004.
111. Kapitsa, A.P., Ridley, J.K., Robin, G. de Q., Siegert, M.J. & Zotikov, I. A. 1996 A large deep freshwater lake beneath the ice of central East Antarctica. Nature, 381, 684-686. doi: 10.1038/381684a0
112. Karr, E.A., Ng, J.M., Belchik, S.M., Sattley, W.M., Madigan, M. T. & Achenbach, L.A. 2003 Biodiversity of methanogenic and other Archaea in the permanently frozen Lake Fryxell, Antarctica. Appl. Environ. Microbiol., 69, 4910.
113. Karr, E.A., Sattley, W.M., Jung, D.O., Madigan, M.T. and Achenbach, L.A. 2003 Remarkable diversity of photo-trophic purple bacteria in a permanently frozen Antarctic lake. Appl. Environ. Microbiol., 69, 4910.
114. Karr, E.A., Ng, J.M., Belchik, S.M., Sattley, W.M., Madigan, M.T. & Achenbach, L.A. 2006 Biodiversity of methano-genic and other Archaea in the permanently Ffrozen Lake Fryxell, Antarctica. Appl. Environ. Microbiol., 72, 1663.
115. Kastovska, K., Elster, J., Stibal, M. & Santruckova, H. 2005 Microbial assemblages in soil microbial succession after glacial retreat in Svalbard (high Arctic). Microb. Ecol., 50, 396.
116. Kawahara, H., Iwanaka, Y., Higa, S., Muryoi, N., Sato, M., Honda, M., Omura, H. & Obata, H. 2007 A novel, intracellular antifreeze protein in an antarctic bacterium, Flavobacterium xanthum. Cryoletters, 28, 39.
117. Kepner, R., Wharton, R. & Suttle, C. 1998 Viruses in Antarctic lakes. Limnol. Oceanogr. 43, 1754.
118. Kepner, R.L., Wharton, R.A. & Coats, D.W. 1999 Ciliated protozoa of two Antarctic lakes: analysis by quantitative protargol staining and examination of artificial substrates. Polar Biol., 21, 285-294. doi: 10.1007/s003000050364
119. Kerr, E.A., Sattley, W.M., Jung, D.O., Madigan, M.T. & Achenbach, L.A. 2003 Remarkable diversity of phototrophic purple bacteria in a permanently frozen Antarctic lake. Appl. Environ. Microbiol., 69, 4910-4914. doi: 10.1128/AEM.69.8.4910-4914.2003
120. Koizumi, Y., Kojima, H. & Fukui, M. 2003 Characterization of depth-related microbial community structure in lake sediment by denaturing gradient gel electrophoresis of amplified 16S rDNA and reversely transcribed 16S rRNA fragments. FEMS Microbiol. Ecol., 46, 147-157. doi: 10.1016/S0168-6496(03)00212-5
121. Komárek, J., Elster, J. & Komarek, O. 2008 Diversity of the cyanobacterial microflora of the northern part of James Ross Island, NW Weddell Sea. Antarctica Polar Biol., 31, 853.
122. Labrenz, M., Lawson, P.A., Tindall, B.J. and Hirsch, P. 2009 Roseibaca ekhonensis. gen. nov., sp. nov., an alkalitolerant and aerobic bacteriochlorophyll a producing alphaproteo-bacterium from hypersaline Ekho Lake. Int. J. Syst. Evol. Microbiol., 59, 1935.
123. Laybourn-Parry, J. & Marchant, H.J. 1992 The microbial plankton of freshwater lakes in the Vestfold Hills, Prince Elizabeth Lane, Antarctica. Polar Biol., 12, 405.
124. Laybourn-Parry, J. & Marshall, W.A. 2003 Photosynthesis, mixotrophy and microbial plankton dynamics in two high Arctic lakes during summer. Polar Biol., 26, 517-524. doi: 10.1007/s00300-003-0514-z
125. Laybourn-Parry, J., Marchant, H.J. & Brown, P.E. 1991 The plankton of a large freshwater Antarctic lake. J. Plankton Res., 13, 1137-1150. (doi: 10.1093/plankt/13.6.1137)
126. Laybourn-Parry, J., Marchant, H.J. & Brown, P.E. 1992 Seasonal cycle of microbial plankton in Crooked Lake. Antarct. Polar Biol., 12, 411.
127. Laybourn-Parry, J., James, M.R., McKnight, D.M., Pricsu, J., Spaulding, S. & Sheil, R. 1997 The microbial plankton of Lake Fryxell, Southern Victoria Land, Antarctica during the summers of 1992 and 1994. Polar Biol., 17, 54-61. doi: 10.1007/s003000050104
128. Laybourn-Parry, J., Quayle, W.C., Henshaw, T., Ruddell, A. & Marchant, H.J. 2001a Life on the edge: the plankton and chemistry of Beaver Lake, an ultra-oligotrophic epishelflake, Antarctica. Freshw. Biol., 46, 1-13. doi: 10.1046/j.1365-2427.2001.00639.x
129. Laybourn-Parry, J., Hofer, J.S. & Sommaruga, R. 2001b Viruses in the plankton of freshwater and saline Antarctic lakes. Freshw. Biol., 46, 1279-1287. doi: 10.1046/j.1365-2427.2001.00749.x
130. Laybourn-Parry, J., Henshaw, T. & Quayle, W.C. 2002 The evolution and biology of Antarctic saline lakes in relation to salinity and trophy. Polar Biol., 25, 542-552. doi: 10.1007/s00300-002-0383-x
131. Laybourn-Parry, J., Marshall, W.A. & Marchant, H.J. 2005 Flagellate nutritional versatility as a key to survival in two contrasting Antarctic saline lakes. Freshw. Biol., 50, 830-838. doi: 10.1111/j.1365-2427.2005.01369.x
132. Laybourn-Parry, J., Marshall, W.A. & Madan, N.J. 2007 Viral dynamics and patterns of lysogeny in saline Antarctic lakes. Polar Biology, 30, 351.
133. Leck, C. & Bigg, E.K. 2005 Biogenic particles in the surface microlayer and overlaying atmosphere in the central Arctic ocean during summer. Tellus Ser. B Chem. Meterorol., 57, 305-316. doi: 10.1111/j.1600-0889.2005.00148.x
134. Li, H-R., Yu, Y., Luo, W. & Zeng, Y-X (2010) Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. Int. J. Syst. Evol. Microbiol., 60, 2535.
135. Lindholm, T. 1985 Mesodinium rubrum.-a unique photosynthetic ciliate. Adv. Aquat. Microbiol., 3, 1.
136. Lisle, J.T. & Priscu, J.C. 2004 The occurrence of lysogenic bacteria and microbial aggregates in the lakes of the McMur-do Dry Valleys, Antarctica. Microb. Ecol., 47, 427-439. doi: 10.1007/s00248-003-1007-x
137. Lizotte, M.P. & Priscu, J.C. 1992 Photosynthesis-irradiance in phytoplankton from the physically stable water column of a perennially ice-covered lake (Lake Bonney, Antarctica). J. Phycol., 28, 179-185. doi: 10.1111/j.0022-3646.1992.00179.x
138. Logares, R., Daugbjerg, N., Boltovskoy, A., Kremp, A., Lay-bourn-Parry, J. & Rengefors, K. 2008 Recent evolutionary diversification of a protist lineage. Environmental Microbiology, 10, 1231.
139. Logares, R., Boltovskoy, A., Bensch, S., Laybourn-Parry, J. & Rengeforsa, K. 2009 Genetic diversity patterns in five protist species occurring in lakes. Protist, 160, 301.
140. Lomolino, M.V. 2000 A call for a new paradigm of island biogeography. Global Ecology and Biogeography, 9, 1.
141. Lopez-Bueno, A., Tamames, J., Velazquez, D., Moya, A., Quesada, A. & Alcami, A. 2009 High diversity of the viral community from an Antarctic lake. Science, 326, 858.
142. Lyons, W.B., Frape, S.K. & Welch, K.A. 1999 History of McMurdo Dry Valley lakes, Antarctica, from stable chlorine isotope data. Geology, 27, 527-530. doi: 10.1130/0091-7613(1999)027!0527:HOMDVLO2.3.CO;2
143. Lyons, W.B., Laybourn-Parry, J. & Welch, K.A. 2007 Antarctic lake systems and climate change. In Trends in Antarctic terrestrial and limnetic systems (eds D. Bergstrom, A. Huiskes & P. Convey), pp. 273-295. Heidelberg, Germany: Springer.
144. MacArthur, R.H. & Wilson, E.O. 1967 The theory of island biogeography.. Princeton, N.J.: Princeton University Press. Madan, N.J., Marshall, W.A. & Laybourn-Parry, J. 2005 Virus and microbial loop dynamics over an annual cycle in three contrasting Antarctic lakes. Freshw. Biol., 50, 1291-1300. doi: 10.1111/j.1365-2427.2005.01399.x
145. Maranger, R. & Bird, D.F. 1995 Viral abundance in aquatic systems-a comparison between marine and fresh-waters. Mar. Ecol. Prog. Ser. 121, 217.
146. Marchant, H.J. 1982 Snow algae from the Australian snowy mountains. Phycologia, 21, 178.
147. Marshall, W.A. 1996 Biological particles over Antarctica. Nature, 383, 680. doi: 10.1038/383680a0
148. Marshall, W. & Laybourn-Parry, J. 2002 The balance between photosynthesis and grazing in Antarctic mixotrophic cryptophytes. Freshw. Biol., 47, 2060-2070. doi: 10.1046/j.1365-2427.2002.00950.x
149. McClaren, I.A. 1964 Zooplankton of Lake Hazen, Ellesmere Island, and a nearby pond with special reference to the copepod Cyclops scutifer Sars. Can. J. Zool., 42, 613.
150. McKnight, D.M., Howes, B.L., Taylor, C.D. & Goehringer, D.D. 2000 Phytoplankton dynamics in a stably stratified Antarctic lake during winter darkness. J. Phycol., 36, 852-861. doi: 10.1046/j.1529-8817.2000.00031.x
151. Médigue, C., Krin, E., Pascal, G., Barbe, V., Bernsel, A., Bertin, P. N., Cheung, F., Cruveiller, S., D'Amico, S., Duilio, A., Fang, G., Feller, G., Ho, C., Mangenot, S., Marino, G., Nilsson, J., Parrilli, E., Rocha, E.P., Rouy, Z., Sekowska, A., Tutino, M.L., Vallenet, D., von Heijne, G. & Danchin A. 2005 Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. Genome Res., 15, 1325.
152. Methé, B.A., Lewis, M., Weaver, B., Weidman, J., Nelson, W., Huston, A., Deming, J. & Fraser, C. 2002 The Colwellia strain 34H genome sequencing project. In: Abstract 142 of the DOE Ninth Genome Sequencing Contractor-Grantee Workshop, Washington, DC, 27-31 January.
153. Methé, B. A. et al. 2005 The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses. Proc. Natl. Acad. Sci. USA, 102, 10,913-10,918. doi: 10.1073/pnas.0504766102
154. Miki, W. 1991 Biological functions and activities of animal caretenoids. Pure Appl. Chem., 63, 141.
155. Mondino, L. J., Asao, M. & Madigan, M.T. 2009 Cold-active halophilic bacteria from the ice-sealed Lake Vida, Antarctica. Archiv. Microbiol., 191, 785.
156. Montes, M. J., Mercade, E., Bozal, N. & Guinea, J. 2004 Paenibacillus antarcticus. sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int. J. Syst. Evol. Microbiol., 54, 1521-1526. doi: 10.1099/ijs.0.63078-0
157. Montes, M.J., Bozal, N. & Mercade, E. 2008 Marinobacterguineae. sp. nov., a novel moderately halophilic bacterium from an Antarctic environment. Int. J. Syst. Evol. Microbiol., 58, 1346.
158. Moosvi, S.A., Pacheco, C.C., McDonald, I.R., De Marco, P., Pearce, D.A., Kelly, D.P. & Wood, A.P. 2005 Isolation and properties of methanesulfonate-degrading Afipia felis from Antarctica and comparison with other strains of A. felis. Environ. Microbiol., 7, 22-33. doi: 10.1111/j.1462-2920.2004.00661.x
159. Morgan, R.M., Ivanov, A.G., Priscu, J.C., Maxwell, D.P. & Hutner, N.P.A. 1998 Structure and composition of the photochemical apparatus of the Antarctic green alga, Chla-mydomonas subcaudata. Photosynth. Res., 56, 303-314. doi: 10.1023/A:1006048519302
160. Mountfort, D.O., Kaspar, H.F., Asher, R.A. & Sutherland, D. 2003 Influences of pond geochemistry, temperature, and freeze-thaw on terminal anaerobic processes occurring in sediments of six ponds of the McMurdo ice shelf, near Bratina Island, Antarctica. Appl. Environ. Microbiol., 69, 583-592. doi: 10.1128/AEM.69.1.583-592.2003
161. Mueller, D.R., Vincent, W.F., Pollard, W.H. & Fritsen, C.H. 2001 Glacial cryoconite ecosystems: a bipolar comparison of algal communities and habitats. In: Algae and extreme environments (eds J. Elster, J. Seckbach, W. F. Vincent & 0. Lhotsky) Nova Hedwigia, Beiheft 123, 173.
162. Mueller, D.R., Vincent, W.F. & Jeffries, M.O. 2003 Ice shelf breakup and ecosystem loss in the Canadian high Arctic. Eos Trans. AGU, 84, 548. doi: 10.1029/2003EO490004
163. Muñoz, J., Felicísimo, M., Cabezas, F., Burgaz, A.R. & Martínez, I. 2004 Wind as a long-distance dispersal vehicle in the southern Hemisphere. Science, 304, 1144-1147. doi: 10.1126/science.1095210
164. Murray, A.E. & Grzymski, J.J. 2007 Diversity and genomics of Antarctic marine micro-organisms. Phil. Trans. R. Soc. B., 362, 2259.
165. Murtagh, G.J., Dyer, P.S., Rogerson, A., Nash, G.V. &Laybourn-Parry, J. 2001 A new species of Tetramitus from an Antarctic saline lake. Eur.J. Protistol., 37, 437-443. doi: 10.1078/0932-4739-00836
166. Muyzer, G., De Waal, E.C. & Uitterlinden, A.G. 1993 Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol., 59, 695.
167. Neale, P.J. & Priscu, J.C. 1995 The photosynthetic apparatus of phytoplankton from a perenially ice-covered lake: acclimation to an extreme shade environment. Plant Cell Physiol., 36, 253.
168. Neale, P.J. & Priscu, J.C. 1998 Fluorescence quenching in phytoplankton of the McMurdo Dry Valley lakes (Antarctica): implications for the structure and function of the photosynthetic apparatus. In Ecosystem dynamics in a polar desert, the McMurdo dry valleys, Antarctica, vol. 72 (ed. J.C. Priscu). Antarctic Research Series, pp. 241-254. Washington, DC: American Geophysical Union.
169. Ng, C., DeMaere, M.Z., Williams, T.J., Lauro, F.M., Raftery, M., Gibson, J.A.E., Andrews-Pfannkoch, C., Lewis, M., Hoffman, J.M., Thomas, T. & Cavicchioli, R. 2010 Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica. The ISME Journal. doi: 10.1038/ismej.2010.28
170. Nichols, D.S., Miller, M.R., Davies, N.W., Goodchild, A., Raftery, M. & Cavicchioli, R. 2004 Cold adaptation in the Antarctic Archaeon Methanococcoides burtonii involves membrane lipid unsaturation J. Bacteriol., 186, 8508.
171. Noon, P.E., Leng, M.J., Arrowsmith, C., Edworthy, M.G. Stra-chan, R.J. 2002 Seasonal observations of stable isotope variations in a valley catchment, Signy Island, South Orkney Islands. Antarct. Sci., 14, 333-342. doi: 10.1017/S0954102002000159
172. Nubel, U., Garcia-Pichel, F. & Muyzer, G. 1997 PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl. Environ. Microbiol., 63, 3327.
173. O'Brien, A., Sharp, R., Russell, N. J. & Roller, S. 2004 Antarctic bacteria inhibit growth of food-borne microorganisms at low temperatures. FEMS Microbiol. Ecol., 48, 157-167. doi: 10.1016/j.femsec.2004.01.001
174. Ochman, H. & Wilson, A.C. 1987 Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. J. Mol. Evol. 26, 74-86. doi: 10.1007/BF02111283
175. Olson, J.B., Steppe, T. Litaker, R.W. & Paerl, H.W. 1998 N_sub_2_/sub_ fixing microbial consortia associated with the ice cover of Lake Bonney, Antarctica. Microb. Ecol., 36, 231-238. doi: 10.1007/s002489900110
176. Oswald, G.K.A. & Robin, G. de Q. 1973 Lakes beneath the Antarctic ice sheet. Nature, 245, 251-254. doi: 10.1038/245251a0
177. Pearce, D.A. 2003 Bacterioplankton community structure in a maritime Antarctic oligotrophic lake during a period of holomixis, as determined by denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridisation (FISH). Microb. Ecol., 46, 92-105. doi: 10.1007/s00248-002-2039-3
178. Pearce, D.A. 2005 The structure and stability of the bacterio-plankton community in Antarctic freshwater lakes, subject to extremely rapid environmental change. FEMS Microbiol. Ecol., 53, 61-72. doi: 10.1016/j.femsec.2005.01.002
179. Pearce, D.A. 2009 Antarctic subglacial lake exploration: anew frontier in microbial ecology. TheISME Journal, 3, 877.
180. Pearce, D.A. & Butler, H.G. 2002 Short-term stability of the microbial community structure in a maritime Antarctic lake. Polar Biol. 25, 479-487. doi: 10.1007/s00300-002-0370-2
181. Pearce, D.A., Van der Gast, C.J., Lawley, B. & Ellis-Evans, J.C. 2003 Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques. FEMS Microbiol. Ecol. 45, 59-70. doi: 10.1016/S0168-6496(03)00110-7
182. Pearce, D.A., van der Gast, C.J., Woodward, K. & Newsham, K. K. 2005 Significant changes in the bacterioplankton community structure of a maritime Antarctic freshwater lake following nutrient enrichment. Microbiol. SGM, 151, 3237-3248. doi: 10.1099/mic.0.27258-0
183. Pearce, D.A., Bridge, P.D., Hughes, K., Sattler, B., Psenner, R. & Russell, N. J. 2009 Microorganisms in the atmosphere over Antarctica. FEMS Microbiology Ecology, 69, 143.
184. Pearce, D.A., Hughes, K.A., Harangozo, S.A., Lachlan-Cope, T.A. & Jones, A.E. 2010 Biodiversity of air-borne microorganisms at Halley station, Antarctica. Extremo-philes, 14, 145.
185. Pearl, H.W. & Priscu, J.C. 1998 Microbial phototrophic, het-erotrophic, and diazotrophic activities associated with aggregates in the permanent ice cover of Lake Bonney, Antarctica. Microb. Ecol., 36, 221-230. doi: 10.1007/s002489900109
186. Peck, L.S. et al. 2005 Genomics: applications to Antarctic ecosystems. Polar Biol., 28, 351-365. doi: 10.1007/s00300-004-0671-8.
187. Pernthaler, A., Pernthaler, J. & Amann, R. 2002 Fluorescence in situ hybridisation and catalysed reporter deposition for the identification of marine bacteria. Appl. Environ. Microbiol., 68, 3094-3101. doi: 10.1128/AEM.68.6.3094-3101.2002
188. Perriss, S.J. & Laybourn-Parry, J. 1997 Microbial communities in the saline lakes of the Vestfold Hills (eastern Antarctica). Polar Biol.18, 135-144. (doi: 10.1007/s003000050168)
189. Perriss, S.J., Laybourn-Parry, J. & Marchant, H.J. 1995 Widespread occurrence of populations of the unique autotrophic ciliate Mesodinium rubrum (Ciliophora: Haptorida) in brackish and saline lakes of the Vestfold Hills (eastern Antarctica). Polar Biol., 14, 423.
190. Pindi, P.K., Kishore, K.H., Reddy, G.S.N. & Shivaji, S. 2009 Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov. Int. J. Syst. Evol. Microbiol., 59, 1348.
191. Pocock, T., Lachance, M.A., Proschold, T., Priscu, J.C., Kim, S.S. & Huner, N.P.A. 2004 Identification of a psychrophilic green alga from Lake Bonney Antarctica: Chlamydomonas rau-densis Ettl. (UWO 241) Chlorophyceae. J. Phycol., 40, 1138-1148. doi: 10.1111/j.1529-8817.2004.04060.x
192. Powell, L.M., Bowman, J.P., Skerratt, J.H., Franzmann, P.D. & Burton, H.R. 2005 Ecology of a novel Synechococcus clade occurring in dense popualtions in saline Antarctic lakes. Mar. Ecol. Prog. Ser., 291, 65.
193. Powell, S.M., Ferguson, S.H., Snape, I. & Sicilliano, S.D. 2006 Fertilization stimulates anaerobic fuel degradation of Antarctic soils by denitrifying microorganisms. Environ. Sci. Technoi., 40, 2011-2017. doi: 10.1021/es051818t
194. Priscu, J.C., Wolf, C.F., Takacs, C.D., Fritsen, C.H., Laybourn-Parry, J., Roberts, E., Sattler, B. & Lyons, W.B. 1999 Carbon transformations in a perennially ice-covered Antarctic lake. BioScience, 49, 997-1008. doi: 10.2307/1313733
195. Purdy, K.J., Hawes, I., Bryant, C.L., Fallick, A.E. & Nedwell, D. B. 2001 Estimates of sulphate reduction ratesin Lake Vanda, Antarctica support the proposed recent history of the lake. Antarct. Sci., 13, 393-399. doi: 10.1017/S0954102001000554
196. Purdy, K.J., Nedwell, D.B. & Embley, T.M. 2003 Analysis of the sulfate-reducing bacterial and methanogenic archaeal populations in contrasting Antarctic sediments. Appl. Environ. Microbiol., 69, 3181-3191. doi: 10.1128/AEM.69.6.3181-3191.2003
197. Quayle, W.C., Peck, L.S., Peat, H., Ellis-Evans, J.C. & Harrigan, P.R. 2002 Extreme responses to climate change in Antarctic lakes. Science 295, 645. doi: 10.1126/science.1064074
198. Ray, M.K., Seshu Kumar, G., Janiyani, K., Kannan, K., Jagtap, P., Basu, M.K. & Shivaji, S. 1998 Adaptation to low temperature and regulation of gene expression in Antarctic psy-chrotrophic bacteria. J. Biosci. 23, 423.
199. Raymond, J.A. & Fritsen, C.H. 2000 Ice-active substances associated with Antarctic freshwater and terrestrial photosynthetic organisms. Antarct. Sci., 12, 418.
200. Raymond, J.A., Christner, B.C. & Schuster, S.C. 2008 A bacterial ice-binding protein from the Vostok ice core. Extremo-philes, 12, 713.
201. Reddy, G.S.N., Prakash, J.S.S., Prabahar, V., Matsumoto, G.I., Stakebrandt, E. & Shivaji, S. 2003a Kocuria polaris sp. nov., an orange pigmented psychrophilic bacterium isolated from and Antarctic cyanobacterial mat sample. Int. J. Syst. Evol. Microbiol., 53, 183-187. doi: 10.1099/ijs.0.02336-0
202. Reddy, G.S.N., Raghavan, P.U.M., Sarita, N.B., Prakash, J.S.S., Nagesh, N., Delille, D. & Shivaji, S. 2003b Halomonas glaciei sp. nov. isolated from fast ice of Adelie Land, Antarctica. Extremophiles, 7, 55.
203. Roberts, D. & McMinn, A. 1999 Palaeohydrological modeling of Ace Lake, Vestfold Hills, Antarctica. Holocene, 9, 401-408. doi: 10.1191/095968399671725699
204. Roberts, D., McMinn, A. & Zwartz, D. 2000 An initial palaeo-salinity history of Jaw Lake, Bunger Hills based on a diatom salinity transfer function applied to sediment cores. Antarct. Sci., 12, 172.
205. Roberts, D., McMinn, A., Cremer, H., Gore, D.B. & Melles, M. 2004a The Holocene evolution and palaeosalinity history of Beall Lake, Windmill Islands (East Antarctica) using an expanded diatom based weighted averaging model. Palaeogeogr. Palaeoclimatol. Palaeoecol., 208, 121-140. doi: 10.1016/j.palaeo.2004.02.032
206. Roberts, E.C., Priscu, J.C., Wolf, C., Lyons, W.B. & Laybourn-Parry, J. 2004b The distribution of microplankton in the McMurdo Dry Valley Lakes, Antarctica: response to ecosystem legacy or present-day climatic controls? Polar Biol., 27, 238-249. doi: 10.1007/s00300-003-0582-0
207. Ruberto, L.A.M., Vazquez, S., Lobalbo, A. & MacCormack, W.P. 2005 Psychrotolerant hydrocarbon-degrading Rhodococ-cus strains isolated from polluted Antarctic soils. Antarct. Sci., 17, 47-56. doi: 10.1017/S0954102005002415
208. Sanders, R.W. & Porter, K.G. 1988 Phagotrophic phytoflagel-lates. Adv. Microb. Ecol., 10, 167.
209. Saunders, N.F.W. et al. 2003 Mechanisms of thermal adaptation revealed from genomes of the Antarctic Archaea Metha-nogenium frigidum and Methanococcoides burtonii. Genome Res., 13, 1580-1588. doi: 10.1101/gr.1180903
210. Säwström C., Anesio, M.A., Granieli, W. & Laybourn-Parry, J. 2007 Seasonal viral loop dynamics in two large ultraoligo-trophic Antarctic freshwater lakes. Microb. Ecol. 53, 1-11. doi: 10.1007/s00248-006-9146-5
211. Säwström C., Lisle, J., Anesio, A.M., Priscu, J.C. & Laybourn-Parry, J. 2008 Bacteriophage in polar inland waters. Extremophiles, 12, 167.
212. Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Esteve, C., Balague, V. & Izaguirre, I. 2009 Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status. Polar Biology, 32, 923.
213. Sheridan, P.P., Loveland-Curtze, J., Miteva, V. I. &Brenchley, J. E. 2003 Rhodoglobus vestalii gen. nov. sp. nov., a novel psychrophilic organism isolated from an Antarctic Dry Valley Lake. Int. J. Syst. Evol. Microbiol., 53, 985-994. doi: 10.1099/ijs.0.02415-0
214. Shravage, B.V., Dayananda, K.M., Patole, M.S. & Shouche, Y.S. 2007 Molecular microbial diversity of a soil sample and detection of ammonia oxidizers from Cape Evans, Mcmurdo Dry Valley, Antarctica. Microbiological Research, 162, 15.
215. Shivaji, S. 2005 Microbial diversity and molecular basis of cold adaptation in Antarctic bacteria. In: Microbial diversity, current perspectives and potential applications, (Eds. T. Satyanarayana & B.N. Johvi) pp 3-24. International publishing House Pvt. Ltd, New Delhi, India.
216. Siegert, M.J., Dowdeswell, J.A., Gorman, M.R. & McIntyre, N.F. 1986 An inventory of Antarctic subglacial lakes. Antarct. Sci., 8, 281.
217. Siegert, M.J., Behar, A., Bentley, M., Blake, D., Bowden, S., Christoffersen, P., Cockell, C., Corr, H., Cullen, D.C., Edwards, H., Ellery, A., Ellis-Evans, J.C., Griffiths, C., Hindmarsh, R., Hodgson, D.A., King, E., Lamb, H., Lane, L., Makinson, K., Mowlem, M., Parnell, J., Pearce, D.A., Priscu, J., Rivera, A., Sephton, M.A., Sims, M.R., Smith, A.M., Tranter, M., Wad-ham, J.L., Wilson, G., & Woodward, J. 2007 Exploration of Ellsworth Subglacial Lake: a concept paper on the development, organisation and execution of an experiment to explore, measure and sample the environment of a West Antarctic subglacial lake. Reviews in Environmental Science and Biotechnology, 6, 161.
218. Simberloff, D. & Wilson, E.O. 1969 Experimental Zoogeography of islands-colonization of empty islands. Ecology, 50, 278.
219. Sjoling, S. & Cowan, D.A. 2000 Detecting human bacterial contamination in Antarctic soils. Polar Biol., 23, 644-650. doi: 10.1007/s003000000137
220. Sjoling. S. & Cowan, D.A. 2003 High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica. Extremophiles, 7, 275.
221. Smith, J.J., Tow, L.A., Stafford, W., Cary, C. & Cowan, D.A. 2006 Bacterial diversity in three different Antarctic cold desert mineral soils. Microbial Ecology, 51, 413.
222. Spaulding, S.A., McKnight, D.M., Smith, R.L. & Dufford, R. 1994 Phytoplankton population-dynamics in perennially ice-covered Lake Fryxell, Antarctica. J. Phytoplankton Res., 16, 527-541. doi: 10.1093/plankt/16.5.527
223. Spigel, R.H. & Priscu, J.C. 1998 Physical limnology of the McMurdo Dry Valley Lakes. In Ecosystem dynamics in a polar desert, the McMurdo Dry Valleys, Antarctica, vol. 72 (ed. J.C. Priscu). Antarctic research series, pp. 153-188. Washington, DC: American Geophysical Union.
224. Spring, S., Merkhoffer, B., Weiss, N., Kroppenstedt, R. M., Hippe, H. & Stakebrandt, E. 2003 Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Chlostridium la-cusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov. Int. J. Syst. Evol. Microbiol., 53, 1019-1029. doi: 10.1099/ijs.0.02554-0
225. Squier, A.H., Hodgson, D.A. & Keely, B.J. 2005 Evidence of late Quaternary environmental change in a continental east Antarctic lake from lacustrine sedimentary pigment distributions. Antarctic Science, 17, 361.
226. Stakebrandt, E., Brambilla, E., Cousin, S., Dirks, W. & Pukall, R. 2004 Culture-independent analysis of bacterial species from an anaerobic mat from Lake Fryxell, Antarctica: Prokaryotic diversity revisited. Cell. Mol. Biol., 50, 517.
227. Storrie-Lombardi, M.C. & Pinkart, H.C. 2007 Co-evolution of cyanophage and cyanobacteria in Antarctic lakes: adaptive responses to high UV flux and global warming. Astrobiology and Planetary Missions X. (eds. R. B. Hoover, G. V. Levin and A. Y. Rozanov). 6694 (54), 1-13.
228. Swadling, K.M., Dartnall, H.J.G., Gibson, J.A.E., Saulnier-Talbot, E. & Vincent, W.F. 2001 Fossil rotifers and the early colonization of an Antarctic lake. Quaternary Res., 55, 380-384. doi: 10.1006/qres.2001.2222
229. Taton, A., Grubisic, S., Brambilla, E., De Wit, R. & Wilmotte, A. 2003 Cyanobacterial diversity in natural and artificial microbial mats of Lake Fyyxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach. Appl. Environ. Microbiol, 69, 5157-5169. doi: 10.1128/AEM.69.9.5157-5169.2003
230. Taton, A., Grubisic, S., Balthasrt, P., Hodgson, D.A., Laybourn-Parry, J. & Wilmotte, A. 2006 Biogeographical distribution and ecological ranges of benthic cyanobacteria in East Antarctic lakes. FEMS Microb. Ecol., 57, 185.
231. Thomas, T., Egan, S., Burg, D., Ng, C., Ting, L. & Cavicchioli, R. 2007 Integration of genomics and proteomics into marine microbial ecology. Mar. Ecol. Prog. Ser. 332, 291.
232. Tindall, B.J. 2004 Prokaryotic diversity in the Antarctic: the tip of the iceberg. Microb. Ecol. 47, 271-283. doi: 10.1007/s00248-003-1050-7
233. Tindall, B.J., Brambilla, E., Steffen, M., Neumann, R., Pukall, R., Kroppenstedt, R.M. & Stackebrandt, E. 2000 Cultivatable microbial biodiversity: gnawing at the Gordian knot. Environ. Microbiol., 72, 310-318. doi: 10.1046/j.1462-2920.2000.00108.x
234. Tranter, M., Fountain, A.G., Fritsen, C.H., Lyons, W.B., Priscu, J.C., Statham, P.J. & Welch, K.A. 2004 Extreme hydrochemical conditions in natural microcosms entombed within Antarctic ice. Hydrol. Process. 18, 379-387. doi: 10.1002/hyp.5217
235. Tranvik, L.J., Porter, K.G. &Sieburth, J.McN. 1989 Occurrence of bacterivory in Cryptomonas, a common freshwater phytoplankter. Oecologia, 78, 473-476. doi: 10.1007/BF00378736
236. Turkiewicz, M., Pazgier, M., Donachie, S.P. & Kalinowska, H. 2006 Invertase and alpha-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcti-cum. Polish Polar Research, 26, 125.
237. Unrein, F., Izaguirre, I., Massana, R., Balague, V. & Gasol, J.M. 2005 Nanoplankton assemblages in maritime Antarctic lakes: characterisation and molecular fingerprinting comparison. Aquat. Microb. Ecol., 40, 269.
238. Van den Burg, B. 2003 Extremophiles as a source for novel enzymes. Curr. Opin. Microbiol., 6, 213-218. doi: 10.1016/S1369-5274(03)00060-2
239. Van den Hoff, J. & Franzmann, P.D. 1986 A choanoflagellate in a hypersaline Antarctic lake. Polar Biol., 6, 71-73. doi: 10.1007/BF00258255
240. Van Trappen, S., Mergaert, J. & Swings, J. 2003 Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int.J. Syst. Evol. Microbiol., 53, 1241-1245. doi: 10.1099/ijs.0.02583-0
241. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. 2004a Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol., 54, 1969-1973. doi: 10.1099/ijs.0.02973-0
242. Van Trappen, S., Mergaert, J. & Swings, J. 2004b Lokanella salsilacus gen. nov., sp. nov., Lokanella fryxellensis sp. nov. and Lokanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol., 54, 1263-1269. doi: 10.1099/ijs.0.03006-0
243. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. 2004c Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov. novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol., 54, 85-92. doi: 10.1099/ijs.0.02857-0
244. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. 2005 Flavobacterium fryxellicola. sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol., 55, 769-772. doi: 10.1099/ijs.0.03056-0
245. Vazquez, S.C., Merino, L.N.R., MacCormack, W.P. & Fraile, E.R. 1995 Protease-producing psychrotrophic bacteria isolated from Antarctica. Polar Biol., 15, 131-135. doi: 10.1007/BF00241051
246. Veillette, J., Mueller, D.R., Antoniades, D. & Vincent, W.F. 2008 Arctic epishelf lakes as sentinel ecosystems: Past, present and future. J. Geophys. Res., 113, G04014, doi: 10.1029/2008JG000730
247. Vincent, W.F. 2000 Evolutionary origins of Antarctic microbiota: invasion, selection and endemism. Antarct. Sci. 12, 374.
248. Vincent, W.F., Bowman, J., Powell, L. & McMeekin, T. 2000 Phylogenetic diversity of picocyanobacteria in Arctic and Antarctic ecosystems. In: Microbial biosystems: new frontiers. Proc. 8 th Int. Symp. on Microbial Ecology (eds. M. Brylinsky, C. Bell & P. Johnson-Green), pp. 317-322. Halifax, Canada: Atlantic Canada Society for Microbial Ecology.
249. Vincent, W.F., Gibson, J.A.E. & Jeffries, M.O. 2001 Ice shelf collapse, climate change and habitat loss in the Canadian high Arctic. Polar Rec., 37, 131.
250. Voytek, M.A., Priscu, J.C. & Ward, B.B. 1999 The distribution and relative abundance of ammonia-oxidizing bacteria in lakes of the McMurdo Dry Valley, Antarctica. Hydrobiologia, 401, 113-130. doi: 10.1023/A:1003754830988
251. Vyverman, W., Verleyen, E., Wilmotte, A., Hodgson, D.A., Willems, A., Peeters, K., Van de Vijver, B., De Wever, A., Leliaert, F. & Sabbe, K. 2010 Evidence for widespread endemism among Antarctic micro-organisms. Polar Sci. 4, 103.
252. Wand, U., Hermichen, W.D., Häfling, R., Mohle, K., Klokov, V. D. & Ufimeev, A.V. 1987 Stable isotopes and hydrogeochemical studies on Beaver Lake and Lake Radok, MacRo-bertson Land, East Antarctica. In: Isotopes in nature (eds. U. Wand & G. Strauch), pp. 99-111. Liepzig, Germany: Academy of Sciences GDR.
253. Weber, A. & Jung, K. 2002 Profiling early osmostress dependent gene expression in Escherichia coli using DNA microarrays. J. Bacteriol., 184, 5502-5507. doi: 10.1128/JB.184.19.5502-5507.2002
254. Wilson, W.H., Lane, D., Pearce, D.A. & Ellis-Evans, J.C. 2000 Transmission electron microscope analysis of virus-like particles in the freshwater lakes of Signy Island, Antarctica. Polar Biol., 23, 657-660. doi: 10.1007/s003000000152
255. Wommack, K.E. & Colwell, R.R. 2000 Virioplankton: viruses in aquatic ecosystems. Microbiol. Mol. Biol. Rev. 64, 69-114. doi: 10.1128/MMBR.64.1.69-114.2000
256. Wood, S.A., Rueckert, A., Cowan, D.A. & Cary, C. 2008a Sources of edaphic cyanobacterial diversity in the Dry Valleys of Eastern Antarctica. The ISME Journal, 2, 308.
257. Wood, S.A., Mountfort, D., Selwood, A.I., Holland, P.T., Puddick, J. & Cary, S.C. 2008b Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats. Appl. Environ. Microbiol., 74, 7243.
258. Xu, H., Griffith, M., Patten, C.L. &Glick, B.R. 1998 Isolation and characterization of an antifreeze protein with ice nucleation activity from the plant growing promoting rhizobacterium Pseudomonas putida GR 12-2. Can. J. Microbiol., 44, 64-73. doi: 10.1139/cjm-44-1-64
259. Yakimov, M.M., Timmis, K.N. & Golyshin, P.N. 2007 Obligate oil-degrading marine bacteria. Current Opinion in Biotechnology, 18, 257.
260. Yang, X.X., Lin, X.Z., Bian, J., Sun, X.Q. & Huang, X.H. 2004 Identification of five strains of Antarctic bacteria producing low-temperature lipase. Acta Oceanol. Sin., 23, 717.
261. Yergeau, E., Schoondermark-Stolk, S.A., Brodie, E.L., Dáejean, S., DeSantis, T.Z., Gonçalves, O., Piceno, Y.M., Andersen, G. L. & Kowalchuk, G.A. 2009 Environmental microarray analyses of Antarctic soil microbial communities, The ISME Journal, 3, 340-351.
262. Yi, H., Oh, H.M., Lee, J.H., Kim, S.J. & Chun, J. 2005a Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int. J. Syst. Evol. Microbiol., 55, 637-641. doi: 10.1099/ijs.0.63423-0
263. Yi, H., Yoon, H. I. & Chun, J. 2005b Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int. J. Syst. Evol. Microbiol., 55, 409-416. doi: 10.1099/ijs.0.63273-0
264. Yu, Y., Li, H.R., Zeng, Y.X. & Chen, B. 2005 Isolation and phylogenetic assignation of actinomycetes in the marine sediments from the Arctic Ocean. Acta Oceanol. Sin. 24, 135.
265. Yu, Y, Xin, Y-H., Liu, H-C., Chen, B., Sheng, J., Chi, Z-M., Zhou, P-J. & Zhang, D-C 2008 Sporosarcina antarctica sp. nov., a psychrophilic bacterium isolated from the Antarctic. Int. J. Syst. Evol. Microbiol., 58, 2114.
266. Zwart, D., Bird, M., Stone, J. & Lambeck, K. 1998 Holocene sea-level change and ice-sheet history in the Vestfold Hills, East Antarctic. Earth Planet. Sci. Lett., 155, 131-145. doi: 10.1016/S0012-821X(97)00204-5
267. Zwart, G., Crump, B.C., van Agterveld, M.P.K., Hagen, F. & Han, S.-K. 2002 Typical freshwater bacteria: an analysis of available 16S rRNA gene sequences from plankton of lakes and rivers. Aquat. Microb. Ecol., 28, 141.