Flow-cytometric method for simultaneous analysis of mouse lung epithelial, endothelial, and hematopoietic lineage cells

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 310, Issue 9, 2016, Pages L796-L801

  Singer, Benjamin D ^a   Mock, Jason R ^a   D’Alessio, Franco R ^a   Aggarwal, Neil R ^a   Mandke, Pooja ^a   Johnston, Laura ^a   Damarla, Mahendra ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cell death; Flow cytometry; Lung; Tissue digestion; Tissue processing

Indexed keywords

7 AMINODACTINOMYCIN; COLLAGENASE; DEOXYRIBONUCLEASE; DISPASE; LIPOPOLYSACCHARIDE; METALLOPROTEINASE;

ANIMAL CELL; ARTICLE; BLOOD VESSEL PERMEABILITY; CELL DEATH; CELL SUSPENSION; CELL VIABILITY; FLOW CYTOMETRY; HEMATOPOIETIC CELL; INTRACELLULAR SPACE; LUNG ALVEOLUS EPITHELIUM; LUNG ENDOTHELIUM; LUNG LAVAGE; MOUSE; NONHUMAN; PRIORITY JOURNAL; ANIMAL; C57BL MOUSE; CELL LINEAGE; CELL SURVIVAL; CYTOLOGY; ENDOTHELIUM CELL; EPITHELIUM CELL; LUNG; MALE; PHYSIOLOGY; PROCEDURES;

ANIMALS; CELL LINEAGE; CELL SURVIVAL; ENDOTHELIAL CELLS; EPITHELIAL CELLS; FLOW CYTOMETRY; LUNG; MALE; MICE, INBRED C57BL;

EID: 84983797292     PISSN: 10400605     EISSN: 15221504     Source Type: Journal    
DOI: 10.1152/ajplung.00334.2015     Document Type: Article

References (16)

1. Autengruber A, Gereke M, Hansen G, Hennig C, Bruder D. Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function. Eur J Microbiol Immunol (Bp) 2: 112–120, 2012.
2. Bantikassegn A, Song X, Politi K. Isolation of epithelial, endothelial, and immune cells from lungs of transgenic mice with oncogene-induced lung adenocarcinomas. Am J Respir Cell Mol Biol 52: 409–417, 2015.
3. Bharat A, Bhorade SM, Morales-Nebreda L, McQuattie-Pimentel AC, Soberanes S, Ridge K, DeCamp MM, Mestan KK, Perlman H, Budinger GRS, Misharin AV. Flow cytometry reveals similarities between lung macrophages in humans and mice. Am J Respir Cell Mol Biol 54: 147–149, 2015.
4. Breu A, Rosenmeier K, Kujat R, Angele P, Zink W. The cytotoxicity of bupivacaine, ropivacaine, and mepivacaine on human chondrocytes and cartilage. Anesth Analg 117: 514–522, 2013.
5. Damarla M, Parniani AR, Johnston L, Maredia H, Serebreni L, Hamdan O, Sidhaye VK, Shimoda LA, Myers AC, Crow MT, Schmidt EP, Machamer CE, Gaestel M, Rane MJ, Kolb TM, Kim BS, Damico RL, Hassoun PM. Mitogen-activated protein kinase-activated protein kinase 2 mediates apoptosis during lung vascular permeability by regulating movement of cleaved caspase 3. Am J Respir Cell Mol Biol 50: 932–941, 2014.
6. Fujino N, Kubo H, Ota C, Suzuki T, Suzuki S, Yamada M, Takahashi T, He M, Suzuki T, Kondo T, Yamaya M. A novel method for isolating individual cellular components from the adult human distal lung. Am J Respir Cell Mol Biol 46: 422–430, 2012.
7. Huang TC, Chen JY. Proteomic analysis reveals that pardaxin triggers apoptotic signaling pathways in human cervical carcinoma HeLa cells: cross talk among the UPR, c-Jun and ROS. Carcinogenesis 34: 1833–1842, 2013.
8. Kawasaki T, Nishiwaki T, Sekine A, Nishimura R, Suda R, Urushibara T, Suzuki T, Takayanagi S, Terada J, Sakao S, Tatsumi K. Vascular repair by tissue-resident endothelial progenitor cells in endotoxin-induced lung injury. Am J Respir Cell Mol Biol 53: 500–512, 2015.
9. Messier EM, Mason RJ, Kosmider B. Efficient and rapid isolation and purification of mouse alveolar type II epithelial cells. Exp Lung Res 38: 363–373, 2012.
10. Mock JR, Garibaldi BT, Aggarwal NR, Jenkins J, Limjunyawong N, Singer BD, Chau E, Rabold R, Files DC, Sidhaye V, Mitzner W, Wagner EM, King LS, D’Alessio FR. Foxp3(+) regulatory T cells promote lung epithelial proliferation. Mucosal Immunol 7: 1440–1451, 2014.
11. Patel BV, Tatham KC, Wilson MR, O’Dea KP, Takata M. In vivo compartmental analysis of leukocytes in mouse lungs. Am J Physiol Lung Cell Mol Physiol 309: L639–L652, 2015.
12. Petit JM, Denis-Gay M, Ratinaud MH. Assessment of fluorochromes for cellular structure and function studies by flow cytometry. Biol Cell 78: 1–13, 1993.
13. Rosich L, Xargay-Torrent S, López-Guerra M, Campo E, Colomer D, Roué G. Counteracting autophagy overcomes resistance to everolimus in mantle cell lymphoma. Clin Cancer Res 18: 5278–5289, 2012.
14. Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y. Fluorescent biomembrane probe for ratiometric detection of apoptosis. J Am Chem Soc 129: 2187–2193, 2007.
15. Singer BD, Mock JR, Aggarwal NR, Garibaldi BT, Sidhaye VK, Florez MA, Chau E, Gibbs KW, Mandke P, Tripathi A, Yegnasubramanian S, King LS, D’Alessio FR. Regulatory T cell DNA methyltransferase inhibition accelerates resolution of lung inflammation. Am J Respir Cell Mol Biol 52: 641–652, 2015.
16. Watanabe M, Hitomi M, van der Wee K, Rothenberg F, Fisher SA, Zucker R, Svoboda KKH, Goldsmith EC, Heiskanen KM, Nieminen AL. The pros and cons of apoptosis assays for use in the study of cells, tissues, and organs. Microsc Microanal 8: 375–391, 2002.