A novel method for isolating individual cellular components from the adult human distal lung

American Journal of Respiratory Cell and Molecular Biology

Volumn 46, Issue 4, 2012, Pages 422-430

  Fujino, Naoya ^a   Kubo, Hiroshi ^a   Ota, Chiharu ^a   Suzuki, Takaya ^a   Suzuki, Satoshi ^b   Yamada, Mitsuhiro ^a   Takahashi, Toru ^a   He, Mei ^a   Suzuki, Takashi ^a   Kondo, Takashi ^a   Yamaya, Mutsuo ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b JAPANESE RED CROSS SOCIETY   (Japan)

Author keywords

Cell culture; Cell isolation; Fluorescence activated cell sorting; Pulmonary alveoli

Indexed keywords

CD45 ANTIGEN; EPITHELIAL CELL ADHESION MOLECULE; PROTEIN ANTIBODY; VASCULAR ENDOTHELIAL CADHERIN;

ARTICLE; CELL ISOLATION; CELL SUSPENSION; CONTROLLED STUDY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG ALVEOLUS CELL TYPE 1; LUNG ALVEOLUS CELL TYPE 2; LUNG PARENCHYMA; LUNG RESECTION;

ADULT; ANTIGENS, CD; ANTIGENS, NEOPLASM; CADHERINS; CELL ADHESION MOLECULES; CELL SEPARATION; CELLS, CULTURED; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; FLOW CYTOMETRY; HUMANS; LUNG; MEMBRANE PROTEINS; MESODERM; PULMONARY ALVEOLI;

EID: 84859413442     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/rcmb.2011-0172OC     Document Type: Article

References (42)

1. Franks TJ, Colby TV, Travis WD, Tuder RM, Reynolds HY, Brody AR, Cardoso WV, Crystal RG, Drake CJ, Engelhardt J, et al. Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function. Proc Am Thorac Soc 2008;5:763-766.
2. Taraseviciene-Stewart L, Voelkel NF. Molecular pathogenesis of emphysema. J Clin Invest 2008;118:394-402. (Pubitemid 351206526)
3. Selman M, King TE, Pardo A. Idiopathic pulmonary fibrosis: prevailing and evolving hypotheses about its pathogenesis and implications for therapy. Ann Intern Med 2001;134:136-151. (Pubitemid 32096342)
4. Mizgerd JP. Acute lower respiratory tract infection. N Engl J Med 2008; 358:716-727.
5. Ware LB, Matthay MA. The acute respiratory distress syndrome. N Engl J Med 2000;342:1334-1349. (Pubitemid 30408719)
6. Yousem SA, Beasley MB. Bronchioloalveolar carcinoma: a review of current concepts and evolving issues. Arch Pathol Lab Med 2007;131: 1027-1032. (Pubitemid 47057889)
7. Fuke S, Betsuyaku T, Nasuhara Y, Morikawa T, Katoh H, Nishimura M. Chemokines in bronchiolar epithelium in the development of chronic obstructive pulmonary disease. Am J Respir Cell Mol Biol 2004;31: 405-412. (Pubitemid 39330950)
8. Suzuki M, Betsuyaku T, Ito Y, Nagai K, Nasuhara Y, Kaga K, Kondo S, Nishimura M. Down-regulated NF-E2-related factor 2 in pulmonary macrophages of aged smokers and patients with chronic obstructive pulmonary disease. Am J Respir Cell Mol Biol 2008;39:673-682.
9. Nagai K, Betsuyaku T, Suzuki M, Nasuhara Y, Kaga K, Kondo S, NishimuraM. Dual oxidase 1 and 2 expression in airway epithelium of smokers and patients with mild/moderate chronic obstructive pulmonary disease. Antioxid Redox Signal 2008;10:705-714. (Pubitemid 351225900)
10. Sirianni FE, Chu FS, Walker DC. Human alveolar wall fibroblasts directly link epithelial Type 2 cells to capillary endothelium. Am J Respir Crit Care Med 2003;168:1532-1537. (Pubitemid 38037341)
11. Okaty BW, Sugino K, Nelson SB. A quantitative comparison of celltype-specific microarray gene expression profiling methods in the mouse brain. PLoS ONE 2011;6:e16493.
12. Fujino N, Ota C, Suzuki T, He M, Takahashi T, Yamada M, Suzuki S, Kondo T, Yamaya M, Kubo H. A new flow-cytometric method for isolating component cells individually from adult human distal lungs. Am J Respir Cell Mol Biol 2011;183:A5100.
13. Fujino N, Kubo H, Suzuki T, Ota C, Hegab AE, He M, Suzuki S, Yamada M, Kondo T, Kato H, et al. Isolation of alveolar epithelial Type II progenitor cells from adult human lungs. Lab Invest 2011;91: 363-378.
14. Fang X, Song Y, Hirsch J, Galietta LJ, Pedemonte N, Zemans RL, Dolganov G, Verkman AS, Matthay MA. Contribution of CFTR to apical-basolateral fluid transport in cultured human alveolar epithelial Type II cells. Am J Physiol Lung Cell Mol Physiol 2006;290:L242-L249.
15. Demling N, Ehrhardt C, Kasper M, Laue M, Knels L, Rieber EP. Promotion of cell adherence and spreading: a novel function of RAGE, the highly selective differentiation marker of human alveolar epithelial Type I cells. Cell Tissue Res 2006;323:475-488. (Pubitemid 43228103)
16. Arnaoutova I, Kleinman HK. In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc 2010;5: 628-635.
17. Hodge SJ, Hodge GL, Holmes M, Reynolds PN. Flow cytometric characterization of cell populations in bronchoalveolar lavage and bronchial brushings from patients with chronic obstructive pulmonary disease. Cytometry B Clin Cytom 2004;61:27-34. (Pubitemid 39223367)
18. Fehrenbach H. Alveolar epithelial Type II cell: defender of the alveolus revisited. Respir Res 2001;2:33-46. (Pubitemid 34187905)
19. Kambouchner M, Bernaudin JF. Intralobular pulmonary lymphatic distribution in normal human lung using D2-40 antipodoplanin immunostaining. J Histochem Cytochem 2009;57:643-648.
20. Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ. Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro. Am J Respir Cell Mol Biol 2007;36:661-668. (Pubitemid 46871420)
21. Kasper M, Behrens J, Schuh D, Muller M. Distribution of E-cadherin and Ep-CAM in the human lung during development and after injury. Histochem Cell Biol 1995;103:281-286.
22. Dobbs LG, Williams MC, Gonzalez R. Monoclonal antibodies specific to apical surfaces of rat alveolar Type I cells bind to surfaces of cultured, but not freshly isolated, Type II cells. Biochim Biophys Acta 1988;970: 146-156.
23. Bikfalvi A, Cramer EM, Tenza D, Tobelem G. Phenotypic modulations of human umbilical vein endothelial cells and human dermal fibroblasts using two angiogenic assays. Biol Cell 1991;72:275-278.
24. Herzog EL, Brody AR, Colby TV, Mason R, Williams MC. Knowns and unknowns of the alveolus. Proc Am Thorac Soc 2008;5:778-782.
25. Crosby LM, Waters CM. Epithelial repair mechanisms in the lung. Am J Physiol Lung Cell Mol Physiol 2010;298:L715-L731.
26. Bingle L, Bull TB, Fox B, Guz A, Richards RJ, Tetley TD. Type II pneumocytes in mixed cell culture of human lung: a light and electron microscopic study. Environ Health Perspect 1990;85:71-80. (Pubitemid 20315283)
27. Cunningham AC, Milne DS, Wilkes J, Dark JH, Tetley TD, Kirby JA. Constitutive expression of MHC and adhesion molecules by alveolar epithelial cells (Type II pneumocytes) isolated from human lung and comparison with immunocytochemical findings. J Cell Sci 1994;107: 443-449. (Pubitemid 24072877)
28. Elbert KJ, Schafer UF, Schafers HJ, Kim KJ, Lee VH, Lehr CM. Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm Res 1999;16:601-608. (Pubitemid 29227086)
29. Herwig MC, Muller KM, Muller AM. Endothelial VE-cadherin expression in human lungs. Pathol Res Pract 2008;204:725-730.
30. Hewett PW, Murray JC. Human lung microvessel endothelial cells: isolation, culture, and characterization. Microvasc Res 1993;46:89-102.
31. Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, Kirkpatrick CJ. Expression of the endothelial markers PECAM-1, vWF, and CD34 in vivo and in vitro. Exp Mol Pathol 2002;72: 221-229.
32. Stevens T, Phan S, Frid MG, Alvarez D, Herzog E, Stenmark KR. Lung vascular cell heterogeneity: endothelium, smooth muscle, and fibroblasts. Proc Am Thorac Soc 2008;5:783-791.
33. El-Chemaly S, Malide D, Zudaire E, Ikeda Y, Weinberg BA, Pacheco- Rodriguez G, Rosas IO, Aparicio M, Ren P, MacDonald SD, et al. Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms. Proc Natl Acad Sci USA 2009;106:3958-3963.
34. Ebina M, Shibata N, Ohta H, Hisata S, Tamada T, Ono M, Okaya K, Kondo T, Nukiwa T. The disappearance of subpleural and interlobular lymphatics in idiopathic pulmonary fibrosis. Lymphat Res Biol 2010;8:199-207.
35. Kumasaka T, Seyama K, Mitani K, Souma S, Kashiwagi S, Hebisawa A, Sato T, Kubo H, Gomi K, Shibuya K, et al. Lymphangiogenesismediated shedding of LAM cell clusters as a mechanism for dissemination in lymphangioleiomyomatosis. Am J Surg Pathol 2005;29: 1356-1366. (Pubitemid 41368772)
36. Tammela T, Alitalo K. Lymphangiogenesis: molecular mechanisms and future promise. Cell 2010;140:460-476.
37. Wigle JT, Oliver G. Prox1 function is required for the development of the murine lymphatic system. Cell 1999;98:769-778. (Pubitemid 29446894)
38. Wigle JT, Harvey N, Detmar M, Lagutina I, Grosveld G, Gunn MD, Jackson DG, Oliver G. An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype. EMBO J 2002;21:1505-1513. (Pubitemid 34614607)
39. Johnson NC, Dillard ME, Baluk P, McDonald DM, Harvey NL, Frase SL, Oliver G. Lymphatic endothelial cell identity is reversible and its maintenance requires Prox1 activity. Genes Dev 2008;22:3282-3291.
40. Baluk P, McDonald DM. Markers for microscopic imaging of lymphangiogenesis and angiogenesis. Ann N Y Acad Sci 2008;1131:1-12. (Pubitemid 351758499)
41. Baluk P, Fuxe J, Hashizume H, Romano T, Lashnits E, Butz S, Vestweber D, Corada M, Molendini C, Dejana E, et al. Functionally specialized junctions between endothelial cells of lymphatic vessels. J Exp Med 2007;204:2349-2362. (Pubitemid 47529321)
42. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science 2010;328:1662-1668.