A microchip for high-throughput axon growth drug screening

Micromachines

Volumn 7, Issue 7, 2016, Pages

  Kim, Hyun Soo ^a   Jeong, Sehoon ^b   Koo, Chiwan ^c   Han, Arum ^a,b   Park, Jaewon ^a,d  

^a Department of Electrical and Computer Engineering   (United States)

^b Texas A M University   (United States)

^c Hanbat National University   (South Korea)

^d SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Compartmentalized culture; High throughput screening; Localized biomolecular treatment; Microfluidic neuron culture platform

Indexed keywords

CELL CULTURE; DRUG PRODUCTS; MICROFLUIDICS; MICROPROCESSOR CHIPS; NEURONS;

AXONAL REGENERATION; BIO-MOLECULAR; CELL CULTURE PLATES; CENTRAL NERVOUS SYSTEMS; HIGH THROUGHPUT; HIGH THROUGHPUT SCREENING; LOCALIZED EFFECTS; NEURON CULTURE;

THROUGHPUT;

EID: 84982803425     PISSN: None     EISSN: 2072666X     Source Type: Journal    
DOI: 10.3390/mi7070114     Document Type: Article

References (40)

1. Qiu, J.; Cai, D.; Filbin, M. Glial inhibition of nerve regeneration in the mature mammalian CNS. Glia 2000, 29, 166-174.
2. Fitch, M.; Silver, J. CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure. Exp. Neurol. 2008, 209, 294-301.
3. Harel, N.; Strittmatter, S. Can regenerating axons recapitulate developmental guidance during recovery from spinal cord injury? Nat. Rev. Neurosci. 2006, 7, 603-616.
4. Yiu, G.; He, Z. Glial inhibition of CNS axon regeneration. Nat. Rev. Neurosci. 2006, 7, 617-627.
5. Park, K.; Liu, K.; Hu, Y.; Smith, P.;Wang, C.; Cai, B.; Xu, B.; Connolly, L.; Kramvis, I.; Sahin, M. Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science 2008, 322, 963-966.
6. Goldberg, J.L.; Espinosa, J.S.; Xu, Y.; Davidson, N.; Kovacs, G.T.; Barres, B.A. Retinal ganglion cells do not extend axons by default: Promotion by neurotrophic signaling and electrical activity. Neuron 2002, 33, 689-702.
7. Goldberg, J.L.; Klassen, M.P.; Hua, Y.; Barres, B.A. Amacrine-signaled loss of intrinsic axon growth ability by retinal ganglion cells. Science 2002, 296, 1860-1864.
8. Hartikka, J.; Hefti, F. Development of septal cholinergic neurons in culture: Plating density and glial cells modulate effects of NGF on survival, fiber growth, and expression of transmitter-specific enzymes. J. Neurosci. 1988, 8, 2967-2985.
9. Brewer, G.; Torricelli, J.; Evege, E.; Price, P. Optimized survival of hippocampal neurons in B27-supplemented neurobasal™, a new serum-free medium combination. J. Neurosci. Res. 1993, 35, 567-576.
10. Ito, D.; Tamate, H.; Nagayama, M.; Uchida, T.; Kudoh, S.; Gohara, K. Minimum neuron density for synchronized bursts in a rat cortical culture on multi-electrode arrays. Neuroscience 2010, 171, 50-61.
11. Robert, F.; Cloix, J.-F.; Hevor, T. Ultrastructural characterization of rat neurons in primary culture. Neuroscience 2012, 200, 248-260.
12. Campenot, R.B. Local control of neurite development by nerve growth factor. Proc. Natl. Acad. Sci. USA 1977, 74, 4516-4519.
13. Ishibashi, T.; Dakin, K.A.; Stevens, B.; Lee, P.R.; Kozlov, S.V.; Stewart, C.L.; Fields, R.D. Astrocytes promote myelination in response to electrical impulses. Neuron 2006, 49, 823-832.
14. Ng, B.K.; Chen, L.; Mandemakers, W.; Cosgaya, J.M.; Chan, J.R. Anterograde transport and secretion of brain-derived neurotrophic factor along sensory axons promote schwann cell myelination. J. Neurosci. 2007, 27, 7597-7603.
15. Taylor, A.M.; Blurton-Jones, M.; Rhee, S.W.; Cribbs, D.H.; Cotman, C.W.; Jeon, N.L. A microfluidic culture platforms for CNS axonal injury, regeneration and transport. Nat. Methods 2005, 2, 599-605.
16. Shi, M.; Majumdar, D.; Gao, Y.; Brewer, B.M.; Goodwin, C.R.; McLean, J.A.; Li, D.; Webb, D.J. Glia co-culture with neurons in microfluidic platforms promotes the formation and stabilization of synaptic contacts. Lab Chip 2013, 13, 3008-3021.
17. Yamaoka, S.; Ito, N.; Ohka, S.; Kaneda, S.; Nakamura, H.; Agari, T.; Masatani, T.; Nakagawa, K.; Okada, K.; Okadera, K. Involvement of rabies virus phosphoprotein gene in neuroinvasiveness. J. Virol. 2013, 87, 12327-12338.
18. Kunze, A.; Lengacher, S.; Dirren, E.; Aebischer, P.; Magistretti, P.J.; Renaud, P. Astrocyte-neuron co-culture on microchips based on the model of SOD mutation to mimic ALS. Integr. Biol. 2013, 5, 964-975.
19. Shi, P.; Scott, M.A.; Ghosh, B.; Wan, D.; Wissner-Gross, Z.; Mazitschek, R.; Haggarty, S.J.; Yanik, M.F. Synapse microarray identification of small molecules that enhance synaptogenesis. Nat. Commun. 2011, 2, 510.
20. Kilinc, D.; Peyrin, J.M.; Soubeyre, V.; Magnifico, S.; Saias, L.; Viovy, J.L.; Brugg, B.Wallerian-like degeneration of central neurons after synchronized and geometrically registered mass axotomy in a three-compartmental microfluidic chip. Neurotox. Res. 2011, 19, 149-161.
21. Hur, E.M.; Yang, I.H.; Kim, D.H.; Byun, J.; Levchenko, A.; Thakor, N.; Zhou, F.-Q. Engineering neuronal growth cones to promote axon regeneration over inhibitory molecules. Proc. Natl. Acad. Sci. USA 2011, 108, 5057-5062.
22. Park, J.W.; Vahidi, B.; Kim, H.J.; Rhee, S.W.; Jeon, N.L. Quantitative analysis of CNS axon regeneration using a microfluidic neuron culture device. Biochip J. 2008, 2, 44-51.
23. Kim, Y.; Karthikeyan, K.; Chirvi, S.; Davé, D. Neuro-optical microfluidic platform to study injury and regeneration of single axons. Lab Chip 2009, 9, 2576-2581.
24. Hosmane, S.; Fournier, A.; Wright, R.; Rajbhandari, L.; Siddique, R.; Yang, I.H.; Ramesh, K.; Venkatesan, A.; Thakor, N. Valve-based microfluidic compression platform: Single axon injury and regrowth. Lab Chip 2011, 11, 3888-3895.
25. Yap, Y.C.; Dickson, T.C.; King, A.E.; Breadmore, M.C.; Guijt, R.M. Microfluidic culture platform for studying neuronal response to mild to very mild axonal stretch injurya. Biomicrofluidics 2014, 8, 044110.
26. Park, J.; Koito, H.; Li, J.; Han, A. Multi-compartment neuron-glia co-culture platform for localized cns axon-glia interaction study. Lab Chip 2012, 12, 3296-3304.
27. Park, J.; Koito, H.; Li, J.; Han, A. Microfluidic compartmentalized co-culture platform for CNS axon myelination research. Biomed. Microdevices 2009, 11, 1145-1153.
28. Park, J.; Li, J.; Han, A. Micro-macro hybrid soft-lithography master (MMHSM) fabrication for lab-on-a-chip applications. Biomed. Microdevices 2010, 12, 345-351.
29. Cho, Y.H.; Park, J.; Park, H.; Cheng, X.; Kim, B.; Han, A. Fabrication of high-aspect-ratio polymer nanochannels using a novel Si nanoimprint mold and solvent-assisted sealing. Microfluid. Nanofluid. 2010, 9, 163-170.
30. Jo, B.H.; Van Lerberghe, L.M.; Motsegood, K.M.; Beebe, D.J. Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer. J. Microelectromech. Syst. 2000, 9, 76-81.
31. Koito, H.; Li, J. Preparation of rat brain aggregate cultures for neuron and glia development studies. J. Vis. Exp. 2009, 31, e1304.
32. Park, J.W.; Vahidi, B.; Taylor, A.; Rhee, S.W.; Jeon, N.L. Microfluidic culture platform for neuroscience research. Nat. Protoc. 2006, 1, 2128-2136.
33. Hengst, U.; Deglincerti, A.; Kim, H.; Jeon, N.; Jaffrey, S. Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein. Nat. Cell Biol. 2009, 11, 1024-1030.
34. Taylor, A.; Dieterich, D.; Ito, H.; Kim, S.; Schuman, E. Microfluidic local perfusion chambers for the visualization and manipulation of synapses. Neuron 2010, 66, 57-68.
35. Dinh, N.D.; Chiang, Y.Y.; Hardelauf, H.; Baumann, J.; Jackson, E.; Waide, S.; Sisnaiske, J.; Frimat, J.P.; van Thriel, C.; Janasek, D.; et al. Microfluidic construction of minimalistic neuronal co-cultures. Lab Chip 2013, 13, 1402-1412.
36. Tong, Z.; Seira, O.; Casas, C.; Reginensi, D.; Homs-Corbera, A.; Samitier, J.; Del Río, J.A. Engineering a functional neuro-muscular junction model in a chip. RSC Adv. 2014, 4, 54788-54797.
37. Park, J.; Kim, S.; Park, S.I.; Choe, Y.; Li, J.; Han, A. A microchip for quantitative analysis of CNS axon growth under localized biomolecular treatments. J. Neurosci. Methods 2014, 221, 166-174.
38. Lingor, P.; Teusch, N.; Schwarz, K.; Mueller, R.; Mack, H.; Bähr, M.; Mueller, B.K. Inhibition of Rho kinase (ROCK) increases neurite outgrowth on chondroitin sulphate proteoglycan in vitro and axonal regeneration in the adult optic nervein vivo. J. Neurochem. 2007, 103, 181-189.
39. Wang, H.; Katagiri, Y.; McCann, T.E.; Unsworth, E.; Goldsmith, P.; Yu, Z.X.; Tan, F.; Santiago, L.; Mills, E.M.; Wang, Y. Chondroitin-4-sulfation negatively regulates axonal guidance and growth. J. Cell Sci. 2008, 121, 3083-3091.
40. Chen, P.; Xu, B.; Tokranova, N.; Feng, X.; Castracane, J.; Gillis, K.D. Amperometric detection of quantal catecholamine secretion from individual cells on micromachined silicon chips. Anal. Chem. 2003, 75, 513-524.