Ultrastructural characterization of rat neurons in primary culture

Neuroscience

Volumn 200, Issue , 2012, Pages 248-260

  Robert, F ^a,b   Cloix, J F ^a   Hevor, T ^a  

^a UNIVERSITÉ D'ORLÉANS   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

Apoptosis; Electron microscopy; Multilamellar vesicles; Neurites; Neuronal growth; Synapses

Indexed keywords

LACTATE DEHYDROGENASE; PENICILLIN G; STREPTOMYCIN;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; BRAIN CORTEX; BRAIN NERVE CELL; CELL ADHESION; CELL COUNT; CELL NUCLEUS; CELL ORGANELLE; CELL SURVIVAL; CELL ULTRASTRUCTURE; CHROMATIN; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; ELECTRON MICROSCOPY; EMBRYO; ENDOPLASMIC RETICULUM; ENZYME RELEASE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INCUBATION TIME; INTRACELLULAR TRANSPORT; LYSOSOME; MICROTUBULE; MITOCHONDRION; MULTILAMELLAR VESICLE; MULTIVESICULAR MYELOID BODY; NERVE CELL CULTURE; NERVE CELL GROWTH; NERVE CELL NECROSIS; NEURITE; NEUROFILAMENT; NONHUMAN; POSTSYNAPTIC MEMBRANE; PRESYNAPTIC MEMBRANE; PRIORITY JOURNAL; RAT; SURVIVAL TIME; SYNAPSE;

ANIMALS; ANTI-BACTERIAL AGENTS; CELL DEATH; CELLS, CULTURED; CEREBRAL CORTEX; EMBRYO, MAMMALIAN; IN SITU NICK-END LABELING; L-LACTATE DEHYDROGENASE; MICROSCOPY, ELECTRON, TRANSMISSION; NEURONS; RATS; TIME FACTORS;

EID: 84855198088     PISSN: 03064522     EISSN: 18737544     Source Type: Journal    
DOI: 10.1016/j.neuroscience.2011.10.002     Document Type: Article

References (39)

1. Alho H., Ferrarese C., Vicini S., Vaccarino F. Subsets of GABAergic neurons in dissociated cell cultures of neonatal rat cerebral cortex show co-localization with specific modulator peptides. Brain Res 1988, 467:193-204.
2. Booher J., Sensenbrenner M. Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 1972, 2:97-105.
3. Brewer G.J. Regeneration and proliferation of embryonic and adult rat hippocampal neurons in culture. Exp Neurol 1999, 159:237-247.
4. Brewer G.J., Boehler M.D., Pearson R.A., DeMaris A.A., Ide A.N., Wheeler B.C. Neuron network activity scales exponentially with synapse density. J Neural Eng 2009, 6:014001.
5. Brewer G.J., Torricelli J.R., Evege E.K., Price P.J. Optimized survival of hippocampal neurons in B27-supplemented neurobasal, a new serum-free medium combination. J Neurosci Res 1993, 35:567-576.
6. Calas A. Les neurones. Physiologie du neurone 1998, 1-29. Doin, Paris. D. Tritsch, D. Chesnoy-Marchais, A. Feltz (Eds.).
7. Cullen D.K., Gilroy M.E., Irons H.R., Laplaca M.C. Synapse-to-neuron ratio is inversely related to neuronal density in mature neuronal cultures. Brain Res 2010, 1359:44-55.
8. De Gois S., Schäfer M.K., Defamie N., Chen C., Ricci A., Weihe E., Varoqui H., Erickson J.D. Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 2005, 25:7121-7133.
9. Delorme P., Hevor T.K. Glycogen particles in methionine sulfoximine epileptogenic rodent brain and liver after the administration of methionine and actinomycin D. Neuropathol Appl Neurobiol 1985, 11:117-128.
10. Dubois-Dauphin M.L., Toni N., Julien S.D., Charvet I., Sundstrom L.E., Stoppini L. The long-term survival of in vitro engineered nervous tissue derived from the specific neural differentiation of mouse embryonic stem cells. Biomaterials 2010, 31:7032-7042.
11. Evans M.S., Collings M.A., Brewer G.J. Electrophysiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture. J Neurosci Methods 1998, 79:37-46.
12. Fattorini G., Verderio C., Melone M., Giovedì S., Benfenati F., Matteoli M., Conti F. VGLUT1 and VGAT are sorted to the same population of synaptic vesicles in subsets of cortical axon terminals. J Neurochem 2009, 110:1538-1546.
13. Fiederling A., Ewert R., Andreyeva A., Jüngling K., Gottmann K. E-cadherin is required at GABAergic synapses in cultured cortical neurons. Neurosci Lett 2011, 501:167-172.
14. Fonseca C.P., Sierra A., Geraldes C.F., Cerdan S., Castro M.M. Mechanisms underlying Li+ effects in glutamatergic and GABAergic neurotransmissions in the adult rat brain and in primary cultures of neural cells as revealed by 13C NMR. J Neurosci Res 2009, 87:1046-1055.
15. Hevor T.K., Delorme P. Biochemical and ultrastructural study of glycogen in cultured astrocytes submitted to the convulsant methionine sulfoximine. Glia 1991, 4:64-69.
16. Kaloyanides G.J. Drug-phospholipid interactions: role in aminoglycoside nephrotoxicity. Ren Fail 1992, 14:351-357.
17. Kato-Negishi M., Muramoto K., Kawahara M., Kuroda Y., Ichikawa M. Developmental changes of GABAergic synapses formed between primary cultured cortical neurons. Brain Res Dev Brain Res 2004, 152:99-108.
18. Kishore B.K., Lambricht P., Ibrahim S., Laurent G., Tulkens P.M., Maldague P. Inhibition of aminoglycoside-induced nephrotoxicity in rats by polyanionic peptides. Contrib Nephrol 1990, 83:191-201.
19. Kivell B.M., McDonald F.J., Miller J.H. Serum-free culture of rat post-natal and fetal brainstem neurons. Brain Res Dev Brain Res 2000, 120:199-210.
20. Kivell B.M., McDonald F.J., Miller J.H. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons. Brain Res Brain Res Protoc 2001, 6:91-99.
21. Klueva J., Meis S., de Lima A.D., Voigt T., Munsch T. Developmental downregulation of GABAergic drive parallels formation of functional synapses in cultured mouse neocortical networks. Dev Neurobiol 2008, 68:934-949.
22. Lasiecka Z.M., Yap C.C., Caplan S., Winckler B. Neuronal early endosomes require EHD1 for L1/NgCAM trafficking. J Neurosci 2010, 30:16485-16497.
23. Laurent G., Kishore B.K., Tulkens P.M. Aminoglycoside-induced renal phospholipidosis and nephrotoxicity. Biochem Pharmacol 1990, 40:2383-2392.
24. Lesuisse C., Martin L.J. Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. J Neurobiol 2002, 51:9-23.
25. Liu M., Nadar V.C., Kozielski F., Kozlowska M., Yu W., Baas P.W. Kinesin-12, a mitotic microtubule-associated motor protein, impacts axonal growth, navigation, and branching. J Neurosci 2010, 30:14896-14906.
26. Louis J.C., Pettmann B., Courageot J., Rumigny J.F., Mandel P., Sensenbrenner M. Developmental changes in cultured neurones from chick embryo cerebral hemispheres. An ultrastructural and neurochemical study. Exp Brain Res 1981, 42:63-72.
27. Marszalec W., Aistrup G.L., Narahashi T. Ethanol modulation of excitatory and inhibitory synaptic interactions in cultured cortical neurons. Alcohol Clin Exp Res 1998, 22:1516-1524.
28. Mohrmann R., Werner M., Hatt H., Gottmann K. Target-specific factors regulate the formation of glutamatergic transmitter release sites in cultured neocortical neurons. J Neurosci 1999, 19:10004-10013.
29. Naval M.V., Gómez-Serranillos M.P., Carretero M.E., De Arce C. Value of high-performance liquid chromatographic analysis of amino acids in the determination of Panax ginseng radix extract effect in cultured neurons. J Chromatogr A 2006, 1121:242-247.
30. Perlman D. Use of antibiotics in cell culture media. Cell Culture 1979, LVIII:110-116. Academic Press, New York. S.P. Colowick, N.O. Kaplan, W.B. Jakoby, I.H. Pastan (Eds.).
31. Pettmann B., Louis J.C., Sensenbrenner M. Morphological and biochemical maturation of neurones cultured in the absence of glial cells. Nature 1979, 281:378-380.
32. Ramakers G.J., van Galen H., Feenstra M.G., Corner M.A., Boer G.J. Activity-dependent plasticity of inhibitory and excitatory amino acid transmitter systems in cultured rat cerebral cortex. Int J Dev Neurosci 1994, 12:611-621.
33. Robert F., Hevor T.K. Abnormal organelles in cultured astrocytes are largely enhanced by streptomycin and intensively by gentamicin. Neuroscience 2007, 144:191-197.
34. Schousboe A., Drejer J., Hansen G.H., Meier E. Cultured neurons as model systems for biochemical and pharmacological studies on receptors for neurotransmitter amino acids. Dev Neurosci 1985, 7:252-262.
35. Sunol C., Babot Z., Fonfria E., Galofre M., Garcia D., Herrera N., Iraola S., Vendrell I. Studies with neuronal cells: from basic studies of mechanisms of neurotoxicity to the prediction of chemical toxicity. Toxicol Vitro 2008, 22:1350-1355.
36. Takei N., Sasaoka K., Inoue K., Takahashi M., Endo Y., Hatanaka H. Brain-derived neurotrophic factor increases the stimulation-evoked release of glutamate and the levels of exocytosis-associated proteins in cultured cortical neurons from embryonic rats. J Neurochem 1997, 68:370-375.
37. Vicini S., Alho H., Costa E., Mienville J.M., Santi M.R., Vaccarino F.M. Modulation of gamma-aminobutyric acid-mediated inhibitory synaptic currents in dissociated cortical cell cultures. Proc Natl Acad Sci U S A 1986, 83:9269-9273.
38. Voigt T., Opitz T., de Lima A.D. Synchronous oscillatory activity in immature cortical network is driven by GABAergic preplate neurons. J Neurosci 2001, 21:8895-8905.
39. Yagami T., Takase K., Yamamoto Y., Ueda K., Takasu N., Okamura N., Sakaeda T., Fujimoto M. Fibroblast growth factor 2 induces apoptosis in the early primary culture of rat cortical neurons. Exp Cell Res 2010, 316:2278-2290.