Mutagenesis of S-adenosyl-L-methionine-binding residues in coronavirus nsp14 N7-methyltransferase demonstrates differing requirements for genome translation and resistance to innate immunity

Journal of Virology

Volumn 90, Issue 16, 2016, Pages 7248-7256

  Case, James Brett ^a   Ashbrook, Alison W ^a   Dermody, Terence S ^a   Denisona, Mark R ^a  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BETA INTERFERON; METHYLTRANSFERASE; MUTAGENIC AGENT; N7 METHYLTRANSFERASE; NSP14 PROTEIN; S ADENOSYLMETHIONINE; UNCLASSIFIED DRUG; VIRUS PROTEIN; ANTIVIRUS AGENT; NONSTRUCTURAL PROTEIN, CORONAVIRUS; VIRAL PROTEIN; VIRUS RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING SITE; IN VITRO STUDY; INNATE IMMUNITY; MURINE HEPATITIS VIRUS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN MOTIF; RNA TRANSLATION; VIRUS GENOME; VIRUS INHIBITION; VIRUS LOAD; VIRUS REPLICATION; VIRUS RESISTANCE; WILD TYPE; AMINO ACID SEQUENCE; ANIMAL; BRAIN TUMOR; C57BL MOUSE; CELL CULTURE; CHEMISTRY; CORONAVIRIDAE; DNA MUTATIONAL ANALYSIS; ENZYMOLOGY; GENETICS; HUMAN; IMMUNOLOGY; IMMUNOMODULATION; KNOCKOUT MOUSE; METABOLISM; MOUSE; MUTATION; PHYSIOLOGY; PROTEIN SYNTHESIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; VIROLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIVIRAL AGENTS; BRAIN NEOPLASMS; CELLS, CULTURED; CORONAVIRUS; DNA MUTATIONAL ANALYSIS; GENOME, VIRAL; HUMANS; IMMUNITY, INNATE; IMMUNOMODULATION; INTERFERON-BETA; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MUTAGENESIS; MUTATION; PROTEIN BIOSYNTHESIS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, VIRAL; S-ADENOSYLMETHIONINE; SEQUENCE HOMOLOGY, AMINO ACID; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 84982214178     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.00542-16     Document Type: Article

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