Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia-Romagna Region (Italy) by qPCR

MicrobiologyOpen

Volumn 5, Issue 4, 2016, Pages 551-559

  Ricchi, Matteo ^a   Savi, Roberto ^a   Bolzoni, Luca ^a   Pongolini, Stefano ^a   Grant, Irene R ^b   De Cicco, Caterina ^a   Cerutti, Giulia ^a   Cammi, Giuliana ^a   Garbarino, Chiara A ^a   Arrigoni, Norma ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA LOMBARDIA E DELL EMILIA ROMAGNA   (Italy)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Bulk tank milk; Mycobacterium avium subsp. paratuberculosis; peptide magnetic separation; qPCR; quantification

Indexed keywords

ARTICLE; BACTERIAL LOAD; BACTERIUM DETECTION; CONCENTRATION (PARAMETERS); DNA EXTRACTION; ITALY; LIMIT OF DETECTION; MILK; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; VALIDATION STUDY;

EID: 84982176719     PISSN: None     EISSN: 20458827     Source Type: Journal    
DOI: 10.1002/mbo3.350     Document Type: Article

References (47)

1. Atreya, R., M. Bülte, G. F. Gerlach, R. Goethe, M. W. Hornef, H. Köhler, et al. Consortium, o.b.o.t.Z. 2014. Facts, myths and hypotheses on the zoonotic nature of Mycobacterium avium subspecies paratuberculosis. Int. J. Med. Microbiol. 304:858–867.
2. Burns, M., and H. Valdivia. 2008. Modelling the limit of detection in real-time quantitative PCR. Eur. Food Res. Technol. 226:12.
3. Bustin, S. A., V. Benes, J. A. Garson, J. Hellemans, J. Huggett, M. Kubista, et al. 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55:611–622.
4. Cammi, G., M. Ricchi, M.N. Losio, R. Savi, E. Cosciani Cunico, N. Arrigoni, et al. 2014. Behaviour of Mycobacterium avium subsp. paratuberculosis (MAP) during manufacturing and aging of Italian hard cheeses. 12th International Colloquium on Paratuberculosis, Parma, Italy. 22/26 June 2014.
5. Carvalho, I. A., A. Silva, V. E. Campos, and M. A. Moreira. 2009. Short communication: detection of Mycobacterium avium subspecies paratuberculosis by polymerase chain reaction in bovine milk in Brazil. J. Dairy Sci. 92:5408–5410.
6. Chiodini, R. J., W. M. Chamberlin, J. Sarosiek, and R. W. McCallum. 2012. Crohn's disease and the mycobacterioses: a quarter century later. Causation or simple association? Crit. Rev. Microbiol. 38:52–93.
7. Cocito, C., P. Gilot, M. Coene, M. de Kesel, P. Poupart, and P. Vannuffel. 1994. Paratuberculosis. Clin. Microbiol. Rev. 7:328–345.
8. Collins, M. T. 2011. Food safety concerns regarding paratuberculosis. Vet. Clin. North Am. Food Anim. Pract. 27:631–636., vii-viii.
9. Corti, S., and R. Stephan. 2002. Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland. BMC Microbiol. 2:15.
10. Cossu, D., E. Cocco, D. Paccagnini, S. Masala, N. Ahmed, J. Frau, et al. 2011. Association of Mycobacterium avium subsp. paratuberculosis with multiple sclerosis in Sardinian patients. PLoS ONE 6:e18482.
11. Donaghy, J. A., J. Johnston, and M. T. Rowe. 2011. Detection of Mycobacterium avium ssp. paratuberculosis in cheese, milk powder and milk using IS900 and f57-based qPCR assays. J. Appl. Microbiol. 110:479–489.
12. Dow, C.T. 2012. M. paratuberculosis Heat Shock Protein 65 and Human Diseases: Bridging Infection and Autoimmunity. Autoimmune Dis. 2012: ID 150824. 6 pages. doi: 10.1155/2012/150824
13. Elguezabal, N., F. Bastida, I. A. Sevilla, N. González, E. Molina, J. M. Garrido, et al. 2011. Estimation of Mycobacterium avium subsp. paratuberculosis growth parameters: strain characterization and comparison of methods. Appl. Environ. Microbiol. 77:8615–8624.
14. Eltholth, M. M., V. R. Marsh, S. Van Winden, and F. J. Guitian. 2009. Contamination of food products with Mycobacterium avium paratuberculosis: a systematic review. J. Appl. Microbiol. 107:1061–1071.
15. Favila-Humara, L. C., G. G. Chávez-Gris, E. M. Carrillo-Casas, and R. Hernández-Castro. 2010. Mycobacterium avium subsp. paratuberculosis detection in individual and bulk tank milk samples from bovine herds and caprine flocks. Foodborne Pathog. Dis. 7:351–355.
16. Foddai, A., C. T. Elliott, and I. R. Grant. 2010. Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells. Appl. Environ. Microbiol. 76:7550–7558.
17. Gardner, I. A. 2004. An epidemiologic critique of current microbial risk assessment practices: the importance of prevalence and test accuracy data. J. Food Prot. 67:2000–2007.
18. Giese, S. B., and P. Ahrens. 2000. Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture. Vet. Microbiol. 77:291–297.
19. Gill, C. O., L. Saucier, and W. J. Meadus. 2011. Mycobacterium avium subsp. paratuberculosis in dairy products, meat, and drinking water. J. Food Prot. 74:480–499.
20. Grant, I.R., and A. Foddai. 2014. Use of the rapid, senstivie and specific PMS-Phage assay reveals the true picture about number of viable Mycobacterium avium subsp. paratuberculosis in raw milk. 12th International Colloquium on Paratuberculosis, Parma, Italy. 22/26 June 2014.
21. Grant, I. R., H. J. Ball, and M. T. Rowe. 2002. Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows’ milk from approved dairy processing establishments in the United Kingdom. Appl. Environ. Microbiol. 68:2428–2435.
22. Haghkhah, M., M. Ansari-Lari, A. M. Novin-Baheran, and A. Bahramy. 2008. Herd-level prevalence of Mycobacterium avium subspecies paratuberculosis by bulk-tank milk PCR in Fars province (southern Iran) dairy herds. Prev. Vet. Med. 86:8–13.
23. Hermon-Taylor, J., and T. Bull. 2002. Crohn's disease caused by Mycobacterium avium subspecies paratuberculosis: a public health tragedy whose resolution is long overdue. J. Med. Microbiol. 51:3–6.
24. Herthnek, D., S. S. Nielsen, A. Lindberg, and G. Bölske. 2008. A robust method for bacterial lysis and DNA purification to be used with real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in milk. J. Microbiol. Methods 75:335–340.
25. Jayarao, B. M., S. R. Pillai, D. R. Wolfgang, D. R. Griswold, C. A. Rossiter, D. Tewari, et al. 2004. Evaluation of IS900-PCR assay for detection of Mycobacterium avium subspecies paratuberculosis infection in cattle using quarter milk and bulk tank milk samples. Foodborne Pathog. Dis. 1:17–26.
26. Kralik, P., V. Beran, and I. Pavlik. 2012. Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry. BMC Res. Notes 5:114.
27. Lee, C., J. Kim, S. G. Shin, and S. Hwang. 2006. Absolute and relative qPCR quantification of plasmid copy number in Escherichia coli. J. Biotechnol. 123:273–280.
28. Logar, K., R. Kopincˇ, P. Bandelj, J. Staricˇ, A. Lapanje, and M. Ocepek. 2012. Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA. BMC Vet. Res. 8:49.
29. Marsh, I., R. Whittington, and D. Cousins. 1999. PCR-restriction endonuclease analysis for identification and strain typing of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium based on polymorphisms in IS1311. Mol. Cell. Probes 13:115–126.
30. Metzger-Boddien, C., D. Khaschabi, M. Schönbauer, S. Boddien, T. Schlederer, and J. Kehle. 2006. Automated high-throughput immunomagnetic separation-PCR for detection of Mycobacterium avium subsp. paratuberculosis in bovine milk. Int. J. Food Microbiol. 110:201–208.
31. Millar, D. S., S. J. Withey, M. L. Tizard, J. G. Ford, and J. Hermon-Taylor. 1995. Solid-phase hybridization capture of low-abundance target DNA sequences: application to the polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum. Anal. Biochem. 226:325–330.
32. Momotani, E., N. M. Romona, K. Yoshihara, Y. Momotani, M. Hori, H. Ozaki, et al. 2012. Molecular pathogenesis of bovine paratuberculosis and human inflammatory bowel. Vet. Immunol. Immunopathol. 148:55–68.
33. International desOffice Epizooties. 2013. Chapter 1.1.5. Principles and methods of validation of diagnostic assays for infectious diseases. OIE Terrestrial Manual. Online: http://www.oie.int/fileadmin/Home/fr/Health_standards/tahm/1.01.05_VALIDATION.pdf Last accessed: 24 February 2016.
34. International desVALIDATION GUIDELINESTATISTICAL APPROACHES TO VALIDATION. OIE Validation GuidelinesOffice Epizooties. 2014. 3.6.5. 2014. Online: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/GUIDELINE_3.6.5_STATISTICAL_VALIDATION.pdf Last accessed: 24 February 2016
35. Okura, H., N. Toft, and S. S. Nielsen. 2012. Occurrence of Mycobacterium avium subsp. paratuberculosis in milk at dairy cattle farms: a systematic review and meta-analysis. Vet. Microbiol. 157:253–263.
36. O'Reilly, C. E., L. O'Connor, W. Anderson, P. Harvey, I. R. Grant, J. Donaghy, et al. 2004. Surveillance of bulk raw and commercially pasteurized cows’ milk from approved Irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosis. Appl. Environ. Microbiol. 70:5138–5144.
37. Rademaker, J. L., M. M. Vissers, and M. C. Te Giffel. 2007. Effective heat inactivation of Mycobacterium avium subsp. paratuberculosis in raw milk contaminated with naturally infected feces. Appl. Environ. Microbiol. 73:4185–4190.
38. Ricchi, M., C. De Cicco, P. Kralik, V. Babak, M. B. Boniotti, R. Savi, et al. 2014. Evaluation of viable Mycobacterium avium subsp paratuberculosis in milk using peptide-mediated separation and Propidium Monoazide qPCR. FEMS Microbiol. Lett. 356:127–133.
39. Scanu, A. M., T. J. Bull, S. Cannas, J. D. Sanderson, L. A. Sechi, G. Dettori, et al. 2007. Mycobacterium avium subspecies paratuberculosis infection in cases of irritable bowel syndrome and comparison with Crohn's disease and Johne's disease: common neural and immune pathogenicities. J. Clin. Microbiol. 45:3883–3890.
40. Serraino, A., P. Bonilauri, N. Arrigoni, F. Ostanello, M. Ricchi, G. Marchetti, et al. 2014. Quantitative risk assessment of Mycobacterium avium subsp. paratuberculosis survival in pasteurized milk in three dairy plants in Italy. Food Control 45:120–126.
41. Sisto, M., L. Cucci, M. D'Amore, T. C. Dow, V. Mitolo, and S. Lisi. 2010. Proposing a relationship between Mycobacterium avium subspecies paratuberculosis infection and Hashimoto's thyroiditis. Scand. J. Infect. Dis. 42:787–790.
42. Slana, I., P. Kralik, A. Kralova, and I. Pavlik. 2008. On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination. Int. J. Food Microbiol. 128:250–257.
43. Slana, I., M. Liapi, M. Moravkova, A. Kralova, and I. Pavlik. 2009. Mycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR. Prev. Vet. Med. 89:223–226.
44. Slaná, I., M. Bartoš, P. Roubal, V. Babák, and I. Pavlík. 2009. Mycobacterium avium subsp. paratuberculosis and M. a. avium detected by culture, IS900 and IS901 highly sensitive PCR in bulk tank milk from dairy herds in the Czech Republic between 2002 and 2004. Czech J. of Food Sci. 27:372–378.
45. Sweeney, R. W., R. H. Whitlock, and A. E. Rosenberger. 1992. Mycobacterium paratuberculosis cultured from milk and supramammary lymph nodes of infected asymptomatic cows. J. Clin. Microbiol. 30:166–171.
46. Vissers, M. M., F. Driehuis, M. C. Te Giffel, P. De Jong, and J. M. Lankveld. 2007. Quantification of the transmission of microorganisms to milk via dirt attached to the exterior of teats. J. Dairy Sci. 90:3579–3582.
47. Weber, M. F., M. Nielen, A. G. Velthuis, and H. J. van Roermund. 2008. Milk quality assurance for paratuberculosis: simulation of within-herd infection dynamics and economics. Vet. Res. 39:12.