A robust method for bacterial lysis and DNA purification to be used with real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in milk

Journal of Microbiological Methods

Volumn 75, Issue 2, 2008, Pages 335-340

  Herthnek, David ^a   Nielsen, Søren Saxmose ^b   Lindberg, Ann ^a   Bölske, Göran ^a  

^a NATIONAL VETERINARY INSTITUTE   (Sweden)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Detection; Johne's disease; Milk; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis; Real time PCR

Indexed keywords

ARTICLE; AUTOANALYSIS; BACTERIOLYSIS; BACTERIUM CULTURE; BACTERIUM DETECTION; CATTLE DISEASE; COLONY FORMING UNIT; CROHN DISEASE; DAIRY CATTLE; DILUTION; DNA EXTRACTION; DNA PURIFICATION; FECES MICROFLORA; HYGIENE; MAGNETIC BEAD SEPARATION; MILK; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM PARATUBERCULOSIS; NONHUMAN; PARATUBERCULOSIS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEPARATION TECHNIQUE;

ANIMALS; BACTERIOLYSIS; CATTLE; CULTURE MEDIA; DNA, BACTERIAL; FECES; IMMUNOMAGNETIC SEPARATION; MILK; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); BOS; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS;

EID: 50549091814     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.07.009     Document Type: Article

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