A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes from the Adult Mouse Heart

Circulation Research

Volumn 119, Issue 8, 2016, Pages 909-920

  Ackers Johnson, Matthew ^a   Li, Peter Yiqing ^a   Holmes, Andrew P ^a   O'Brien, Sian Marie ^b   Pavlovic, Davor ^b   Foo, Roger S ^c  

^a NATIONAL UNIVERSITY HEALTH SYSTEM   (Singapore)

^b GENOME INSTITUTE OF SINGAPORE   (Singapore)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

cardiac fibroblasts; cardiomyocytes; cardiovascular disease; coculture; Langendorff free; mouse models; single cell isolation

Indexed keywords

ANTIBIOTIC AGENT; CALCIUM; HEPARIN;

ADULT; ANIMAL CELL; ARTICLE; CARDIAC MUSCLE CELL; CELL CULTURE; COCULTURE; CONTROLLED STUDY; EX VIVO STUDY; GENE TRANSFER; HEART; HYPOXIA; ISOLATED HEART; LABORATORY DEVICE; MITOCHONDRION; MOUSE; NONHUMAN; PATCH CLAMP TECHNIQUE; PRIORITY JOURNAL; SURGICAL EQUIPMENT; ANIMAL; C57BL MOUSE; CARDIAC MUSCLE; CELL CULTURE TECHNIQUE; CELL SEPARATION; CYTOLOGY; FIBROBLAST; HEART VENTRICLE; PHYSIOLOGY; PROCEDURES;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SEPARATION; COCULTURE TECHNIQUES; FIBROBLASTS; HEART VENTRICLES; ISOLATED HEART PREPARATION; MICE; MICE, INBRED C57BL; MYOCARDIUM; MYOCYTES, CARDIAC;

EID: 84981742810     PISSN: 00097330     EISSN: 15244571     Source Type: Journal    
DOI: 10.1161/CIRCRESAHA.116.309202     Document Type: Article

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