Standardized bioenergetic profiling of adult mouse cardiomyocytes

Physiological Genomics

Volumn 44, Issue 24, 2012, Pages 1208-1213

  Readnower, Ryan D ^a   Brainard, Robert E ^a   Hill, Bradford G ^a   Jones, Steven P ^a  

^a University of Louisville School of Medicine   (United States)

Author keywords

Fatty acid oxidation; Glycolysis; Metabolism; Mitochondria; Respiration

Indexed keywords

FATTY ACID; GLUCOSE; LAMININ; PALMITIC ACID; PYRUVIC ACID;

ANALYSIS; ANIMAL CELL; ARTICLE; BIOENERGY; CELL DENSITY; CELL METABOLISM; CELL STRUCTURE; CONTROLLED STUDY; DISORDERS OF MITOCHONDRIAL FUNCTIONS; ENERGY YIELD; EXTRACELLULAR FLUX; HEART FAILURE; HEART MUSCLE CELL; HEART MUSCLE ISCHEMIA; HEART MUSCLE OXYGEN CONSUMPTION; MALE; MOUSE; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL;

AGING; ANIMALS; CELL COUNT; ENERGY METABOLISM; EXTRACELLULAR SPACE; MALE; METABOLOMICS; MICE; MICE, INBRED C57BL; MITOCHONDRIA, HEART; MYOCYTES, CARDIAC; PALMITATES; PYRUVATES; REFERENCE STANDARDS;

EID: 84871542802     PISSN: 10948341     EISSN: 15312267     Source Type: Journal    
DOI: 10.1152/physiolgenomics.00129.2012     Document Type: Article

References (29)

1. Abe Y, Sakairi T, Kajiyama H, Shrivastav S, Beeson C, Kopp JB. Bioenergetic characterization of mouse podocytes. Am J Physiol Cell Physiol 299: C464-C476, 2010.
2. Abel ED. Glucose transport in the heart. Front Biosci 9: 201-215, 2004.
3. Brand MD, Nicholls DG. Assessing mitochondrial dysfunction in cells. Biochem J 435: 297-312, 2011.
4. Chance B, Williams GR. Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217: 383-393, 1955.
5. Dranka BP, Benavides GA, Diers AR, Giordano S, Zelickson BR, Reily C, Zou L, Chatham JC, Hill BG, Zhang J, Landar A, Darley-Usmar VM. Assessing bioenergetic function in response to oxidative stress by metabolic profiling. Free Radic Biol Med 51: 1621-1635, 2011.
6. Dranka BP, Hill BG, Darley-Usmar VM. Mitochondrial reserve capacity in endothelial cells: the impact of nitric oxide and reactive oxygen species. Free Radic Biol Med 48: 905-914, 2010.
7. Fang YH, Piao L, Hong Z, Toth PT, Marsboom G, Bache-Wiig P, Rehman J, Archer SL. Therapeutic inhibition of fatty acid oxidation in right ventricular hypertrophy: exploiting Randle's cycle. J Mol Med 90: 31-43, 2012.
8. Gropler RJ, Beanlands RS, Dilsizian V, Lewandowski ED, Villanueva FS, Ziadi MC. Imaging myocardial metabolic remodeling. J Nucl Med 51, Suppl 1: 88S-101S, 2010.
9. Guo S, Olm-Shipman A, Walters A, Urciuoli WR, Devito S, Nadtochiy SM, Wojtovich AP, Brookes PS. A cell-based phenotypic assay to identify cardioprotective agents. Circ Res 110: 948-957, 2012.
10. Hill BG, Dranka BP, Zou L, Chatham JC, Darley-Usmar VM. Importance of the bioenergetic reserve capacity in response to cardiomyocyte stress induced by 4-hydroxynonenal. Biochem J 424: 99-107, 2009.
11. Hill BG, Higdon AN, Dranka BP, Darley-Usmar VM. Regulation of vascular smooth muscle cell bioenergetic function by protein glutathiolation. Biochim Biophys Acta 1797: 285-295, 2010.
12. Louch WE, Sheehan KA, Wolska BM. Methods in cardiomyocyte isolation, culture, and gene transfer. J Mol Cell Cardiol 51: 288-298, 2011.
13. Luo J, Hill BG, Gu Y, Cai J, Srivastava S, Bhatnagar A, Prabhu SD. Mechanisms of acrolein-induced myocardial dysfunction: implications for environmental and endogenous aldehyde exposure. Am J Physiol Heart Circ Physiol 293: H3673-H3684, 2007.
14. Matsumoto T, Wang PY, Ma W, Sung HJ, Matoba S, Hwang PM. Polo-like kinases mediate cell survival in mitochondrial dysfunction. Proc Natl Acad Sci USA 106: 14542-14546, 2009.
15. McGarry JD, Brown NF. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. Eur J Biochem 244: 1-14, 1997.
16. Nicholls DG, Darley-Usmar VM, Wu M, Jensen PB, Rogers GW, Ferrick DA. Bioenergetic profile experiment using C2C12 myoblast cells. J Visual Exp: e2511, 2010.
17. Powell T, Twist VW. A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium. Biochem Biophys Res Commun 72: 327-333, 1976.
18. Readnower RD, Pandya JD, McEwen ML, Pauly JR, Springer JE, Sullivan PG. Post-injury administration of the mitochondrial permeability transition pore inhibitor, NIM811, is neuroprotective and improves cognition after traumatic brain injury in rats. J Neurotrauma 28: 1845-1853, 2011.
19. Rustin P, Chretien D, Bourgeron T, Wucher A, Saudubray JM, Rotig A, Munnich A. Assessment of the mitochondrial respiratory chain. Lancet 338: 60, 1991.
20. Sansbury BE, Jones SP, Riggs DW, Darley-Usmar VM, Hill BG. Bioenergetic function in cardiovascular cells: the importance of the reserve capacity and its biological regulation. Chemico-biol Interact 191: 288-295, 2011.
21. Sansbury BE, Riggs DW, Brainard RE, Salabei JK, Jones SP, Hill BG. Responses of hypertrophied myocytes to reactive species: implications for glycolysis and electrophile metabolism. Biochem J 435: 519-528, 2011.
22. Sauerbeck A, Pandya J, Singh I, Bittman K, Readnower R, Bing G, Sullivan P. Analysis of regional brain mitochondrial bioenergetics and susceptibility to mitochondrial inhibition utilizing a microplate based system. J Neurosci Meth 198: 36-43, 2011.
23. Shipp JC. Interrelation between carbohydrate and fatty acid metabolism of isolated perfused rat heart. Metab Clin Exp 13: 852-867, 1964.
24. Sparagna GC, Hickson-Bick DL, Buja LM, McMillin JB. A metabolic role for mitochondria in palmitate-induced cardiac myocyte apoptosis. Am J Physiol Heart Circ Physiol 279: H2124-H2132, 2000.
25. Sugden MC, Holness MJ. Recent advances in mechanisms regulating glucose oxidation at the level of the pyruvate dehydrogenase complex by PDKs. Am J Physiol Endocrinol Metab 284: E855-E862, 2003.
26. Taegtmeyer H, Golfman L, Sharma S, Razeghi P, van Arsdall M. Linking gene expression to function: metabolic flexibility in the normal and diseased heart. Ann NY Acad Sci 1015: 202-213, 2004.
27. Taegtmeyer H, Sen S, Vela D. Return to the fetal gene program: a suggested metabolic link to gene expression in the heart. Ann NY Acad Sci 1188: 191-198, 2010.
28. Watanabe M, Houten SM, Mataki C, Christoffolete MA, Kim BW, Sato H, Messaddeq N, Harney JW, Ezaki O, Kodama T, Schoonjans K, Bianco AC, Auwerx J. Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation. Nature 439: 484-489, 2006.
29. Wolska BM, Solaro RJ. Method for isolation of adult mouse cardiac myocytes for studies of contraction and microfluorimetry. Am J Physiol Heart Circ Physiol 271: H1250-H1255, 1996.