Optimal lentivirus production and cell culture conditions necessary to successfully transduce primary human bronchial epithelial cells

Journal of Visualized Experiments

Volumn 2016, Issue 113, 2016, Pages

  Baumlin Schmid, Nathalie ^a   Salathe, Matthias ^a   Fregien, Nevis L ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

Airliquid interface medium; Bronchial epithelial growth medium; Issue 113; Lentiviral vector; Microbiology; Multiplicity of infection; Primary human bronchial epithelial cells; Transduction

Indexed keywords

BRONCHUS; CELL CULTURE TECHNIQUE; CELL DIFFERENTIATION; CYTOLOGY; EPITHELIUM CELL; GENE VECTOR; GENETIC TRANSDUCTION; GROWTH, DEVELOPMENT AND AGING; HUMAN; LENTIVIRINAE; PROCEDURES; VIROLOGY; VIRUS CULTURE;

BRONCHI; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; EPITHELIAL CELLS; GENETIC VECTORS; HUMANS; LENTIVIRUS; TRANSDUCTION, GENETIC; VIRUS CULTIVATION;

EID: 84979915638     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/54176     Document Type: Article

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