Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Cribbs, Adam P ^a   Kennedy, Alan ^a   Gregory, Bernard ^a   Brennan, Fionula M ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

CD45RA+; Primary lymphocytes; Titre; Ultracentrifugation

Indexed keywords

CD45RA; PRIMARY HUMAN T CELLS; PRIMARY LYMPHOCYTES; SIMPLE MODIFICATIONS; TITRE; TRANSDUCTION EFFICIENCY; TRANSGENE EXPRESSION; ULTRACENTRIFUGATION;

CELLS; CYTOLOGY; EFFICIENCY; GENE TRANSFER;

BACTERIOPHAGES;

CD45RA ANTIGEN; LENTIVIRUS VECTOR;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HUMAN; HUMAN CELL; LENTIVIRINAE; NONHUMAN; PURIFICATION; T LYMPHOCYTE; TITRIMETRY; ULTRACENTRIFUGATION; ULTRACENTRIFUGE; VIRAL GENE DELIVERY SYSTEM;

LENTIVIRUS;

ANTIGENS, CD45; BUTYRIC ACID; CALCIUM PHOSPHATES; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HEK293 CELLS; HUMANS; LENTIVIRUS; LIPIDS; PLASMIDS; T-LYMPHOCYTES; TRANSDUCTION, GENETIC; TRANSFECTION; TRANSGENES;

EID: 84887293605     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-98     Document Type: Article

References (24)

1. Young LS, Searle PF, Onion D, Mautner V. Viral gene therapy strategies: from basic science to clinical application. J Pathol 2006, 208(2):299-318. 10.1002/path.1896, 16362990.
2. Biffi A, Naldini L. Gene therapy of storage disorders by retroviral and lentiviral vectors. Hum Gene Ther 2005, 16(10):1133-1142. 10.1089/hum.2005.16.1133, 16218774.
3. Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 1996, 272(5259):263-267. 10.1126/science.272.5259.263, 8602510.
4. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. A third-generation lentivirus vector with a conditional packaging system. J Virol 1998, 72(11):8463-8471. 110254, 9765382.
5. Kutner RH, Zhang XY, Reiser J. Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors. Nat Protoc 2009, 4(4):495-505. 10.1038/nprot.2009.22, 19300443.
6. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. Optimized large-scale production of high titer lentivirus vector pseudotypes. J Virol Methods 2004, 122(2):131-139. 10.1016/j.jviromet.2004.08.017, 15542136.
7. Reiser J. Production and concentration of pseudotyped HIV-1-based gene transfer vectors. Gene Ther 2000, 7(11):910-913. 10.1038/sj.gt.3301188, 10849549.
8. Baekelandt V, Eggermont K, Michiels M, Nuttin B, Debyser Z. Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain. Gene Ther 2003, 10(23):1933-1940. 10.1038/sj.gt.3302094, 14528317.
9. al Yacoub N, Romanowska M, Haritonova N, Foerster J. Optimized production and concentration of lentiviral vectors containing large inserts. J Gene Med 2007, 9(7):579-584. 10.1002/jgm.1052, 17533614.
10. Zhang B, Xia HQ, Cleghorn G, Gobe G, West M, Wei MQ. A highly efficient and consistent method for harvesting large volumes of high-titre lentiviral vectors. Gene therapy 2001, 8(22):1745-1751. 10.1038/sj.gt.3301587, 11892843.
11. Swainson L, Mongellaz C, Adjali O, Vicente R, Taylor N. Lentiviral transduction of immune cells. Methods Mol Biol 2008, 415:301-320.
12. Verhoeyen E, Costa C, Cosset FL. Lentiviral vector gene transfer into human T cells. Methods Mol Biol 2009, 506:97-114. 10.1007/978-1-59745-409-4_8, 19110622.
13. Zack JA, Kim SG, Vatakis DN. HIV restriction in quiescent CD4(+) T cells. Retrovirology 2013, 10:37. 10.1186/1742-4690-10-37, 3626626, 23557201.
14. Korin YD, Zack JA. Progression to the G1b phase of the cell cycle is required for completion of human immunodeficiency virus type 1 reverse transcription in T cells. J Virol 1998, 72(4):3161-3168. 109773, 9525642.
15. Amendola M, Venneri MA, Biffi A, Vigna E, Naldini L. Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters. Nat Biotechnol 2005, 23(1):108-116. 10.1038/nbt1049, 15619618.
16. Allan SE, Alstad AN, Merindol N, Crellin NK, Amendola M, Bacchetta R, Naldini L, Roncarolo MG, Soudeyns H, Levings MK. Generation of potent and stable human CD4+ T regulatory cells by activation-independent expression of FOXP3. Mol Therapy 2008, 16(1):194-202.
17. Gorman CM, Howard BH, Reeves R. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res 1983, 11(21):7631-7648. 10.1093/nar/11.21.7631, 326508, 6316266.
18. Sakoda T, Kasahara N, Hamamori Y, Kedes L. A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J Mol Cell Cardiol 1999, 31(11):2037-2047. 10.1006/jmcc.1999.1035, 10591030.
19. Case SS, Price MA, Jordan CT, Yu XJ, Wang L, Bauer G, Haas DL, Xu D, Stripecke R, Naldini L, et al. Stable transduction of quiescent CD34(+)CD38(-) human hematopoietic cells by HIV-1-based lentiviral vectors. Proc Natl Acad Sci USA 1999, 96(6):2988-2993. 10.1073/pnas.96.6.2988, 15882, 10077624.
20. Levin MC, Lidberg U, Jirholt P, Adiels M, Wramstedt A, Gustafsson K, Greaves DR, Li S, Fazio S, Linton MF, et al. Evaluation of macrophage-specific promoters using lentiviral delivery in mice. Gene Ther 2012, 19(11):1041-1047. 10.1038/gt.2011.195, 3697098, 22130447.
21. Siwko SK, Bu W, Gutierrez C, Lewis B, Jechlinger M, Schaffhausen B, Li Y. Lentivirus-mediated oncogene introduction into mammary cells in vivo induces tumors. Neoplasia 2008, 10(7):653-662. 651 p following 662, 2435599, 18592025.
22. Ichim CV, Wells RA. Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation. J Transl Med 2011, 9:137. 10.1186/1479-5876-9-137, 3175463, 21849073.
23. Al Yacoub N, Romanowska M, Truss M, Haritonova N, Foerster J. A widely applicable optimized protocol for lentivirus production and virus concentration. Exp Dermatol 2007, 16(3):286-286.
24. Maurice M, Verhoeyen E, Salmon P, Trono D, Russell SJ, Cosset FL. Efficient gene transfer into human primary blood lymphocytes by surface-engineered lentiviral vectors that display a T cell-activating polypeptide. Blood 2002, 99(7):2342-2350. 10.1182/blood.V99.7.2342, 11895766.