A sensitive and robust high-throughput screening assay for inhibitors of the Chikungunya virus nsP1 capping enzyme

PLoS ONE

Volumn 11, Issue 7, 2016, Pages

  Bullard Feibelman, Kristen M ^a   Fuller, Benjamin P ^a   Geiss, Brian J ^a,b  

^a Department of Environmental and Radiological Health Sciences   (United States)

^b Engineering Research Center and Hydraulics Laboratory   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE PHOSPHATE; GUANOSINE TRIPHOSPHATE; GUANYLYLTRANSFERASE; METHYLTRANSFERASE; PROTEIN NSP1; TRANSFERASE; UNCLASSIFIED DRUG; VIRUS ENZYME; VIRUS RNA; ANTIVIRUS AGENT; CAPPED RNA; ENZYME INHIBITOR; NUCLEOTIDYLTRANSFERASE;

ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; FLUORESCENCE ANALYSIS; FLUORESCENCE POLARIZATION; GUANYLATION; HIGH THROUGHPUT SCREENING; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SENSITIVITY ANALYSIS; STRUCTURE ANALYSIS; VALIDATION PROCESS; ANTAGONISTS AND INHIBITORS; CHEMISTRY; CHIKUNGUNYA FEVER; CHIKUNGUNYA VIRUS; DRUG EFFECTS; ENZYMOLOGY; HUMAN; METABOLISM; PROCEDURES; VIROLOGY;

ANTIVIRAL AGENTS; CHIKUNGUNYA FEVER; CHIKUNGUNYA VIRUS; ENZYME INHIBITORS; GUANOSINE TRIPHOSPHATE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; METHYLTRANSFERASES; NUCLEOTIDYLTRANSFERASES; RNA CAPS;

EID: 84979307917     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0158923     Document Type: Article

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