Enhanced production of nargenicin A1 and creation of a novel derivative using a synthetic biology platform

Applied Microbiology and Biotechnology

Volumn 100, Issue 23, 2016, Pages 9917-9931

  Dhakal, Dipesh ^a   Chaudhary, Amit Kumar ^a   Yi, Jeong Sang ^b   Pokhrel, Anaya Raj ^a   Shrestha, Biplav ^a   Parajuli, Prakash ^a   Shrestha, Anil ^a   Yamaguchi, Tokutaro ^a   Jung, Hye Jin ^a   Kim, Seung Young ^a   Kim, Byung Gee ^b   Sohng, Jae Kyung ^a  

^a SunMoon University   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Bioactivity; Multi monocistronic vector; Nargenicin A1 acid; Nocardia; Synthetic biology

Indexed keywords

BACTERIA; BIOACTIVITY; BIOLOGY; CLONING; GENES; GENETIC ENGINEERING; GLUCOSE; MASS SPECTROMETRY; METABOLIC ENGINEERING; NUCLEAR MAGNETIC RESONANCE;

ANTI-BACTERIAL ACTIVITY; NOCARDIA; NUCLEAR MAGNETIC RESONANCE(NMR); STATISTICAL MEDIA OPTIMIZATION; STATISTICAL OPTIMIZATION; STREPTOMYCES COELICOLOR A3(2); STREPTOMYCES VENEZUELAE; SYNTHETIC BIOLOGY;

BINDING SITES;

ANTIINFECTIVE AGENT; MALONYL COENZYME A; NARGENICIN A1; PYRROLE DERIVATIVE; UNCLASSIFIED DRUG; CULTURE MEDIUM; LACTONE; RECOMBINANT PROTEIN;

ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOACTIVITY; BIOASSAY; BIOLOGICAL PRODUCTION; BIOLOGY; CHEMICAL COMPOUND; GENE; GENETIC ENGINEERING;

ARTICLE; BACTERIAL GROWTH; BINDING SITE; DRUG MANUFACTURE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME SYNTHESIS; GENE EXPRESSION; GENETIC ENGINEERING; GLUCOSE TRANSPORT; GLUCOSE UTILIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLIC ENGINEERING; MOLECULAR CLONING; NOCARDIA; NONHUMAN; PRECURSOR; PROCESS OPTIMIZATION; PROMOTER REGION; RESTRICTION SITE; RIBOSOME; STREPTOMYCES COELICOLOR; SYNTHETIC BIOLOGY; ZYMOMONAS MOBILIS; CHEMISTRY; CULTURE MEDIUM; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM;

NOCARDIA; NOCARDIA SP.; POSIBACTERIA; STREPTOMYCES COELICOLOR; STREPTOMYCES VENEZUELAE; ZYMOMONAS MOBILIS;

ANTI-BACTERIAL AGENTS; CULTURE MEDIA; GENE EXPRESSION; GENETIC VECTORS; LACTONES; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; NOCARDIA; RECOMBINANT PROTEINS; SYNTHETIC BIOLOGY;

EID: 84978087336     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-016-7705-3     Document Type: Article

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