메뉴 건너뛰기




Volumn 2016, Issue 112, 2016, Pages

Mucin agarose gel electrophoresis: Western blotting for high-molecularweight glycoproteins

Author keywords

Double staining; Electrophoresis; Fluorescence; High molecular weight glycoproteins; Issue 112; Molecular Biology; Mucins; Nitrocellulose membrane; SDS agarose gel; Vacuum blotter

Indexed keywords

ANTIBODY; ARTIFICIAL MEMBRANE; MUCIN; PYROXYLIN;

EID: 84977565442     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/54153     Document Type: Article
Times cited : (27)

References (19)
  • 1
    • 84903759350 scopus 로고    scopus 로고
    • Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure
    • Henderson, A. G. et al. Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure. J Clin Invest. 124 (7), 3047-3060 (2014).
    • (2014) J Clin Invest , vol.124 , Issue.7 , pp. 3047-3060
    • Henderson, A.G.1
  • 2
    • 77955623546 scopus 로고    scopus 로고
    • MUC5B Is the predominant mucin glycoprotein in chronic otitis media fluid
    • Preciado, D. et al. MUC5B Is the predominant mucin glycoprotein in chronic otitis media fluid. Pediatr Res. 68 (3), 231-236 (2010).
    • (2010) Pediatr Res , vol.68 , Issue.3 , pp. 231-236
    • Preciado, D.1
  • 3
    • 84899806985 scopus 로고    scopus 로고
    • IgG in cervicovaginal mucus traps HSV and prevents vaginal herpes infections
    • Wang, Y. Y. et al. IgG in cervicovaginal mucus traps HSV and prevents vaginal herpes infections. Mucosal Immunol. 7 (5), 1036-1044 (2014).
    • (2014) Mucosal Immunol , vol.7 , Issue.5 , pp. 1036-1044
    • Wang, Y.Y.1
  • 5
    • 0025138569 scopus 로고
    • Mucus glycoproteins from ‘normal’ human tracheobronchial secretion
    • Thornton, D. J. et al. Mucus glycoproteins from ‘normal’ human tracheobronchial secretion. Biochem J. 265 (1), 179-186 (1990).
    • (1990) Biochem J , vol.265 , Issue.1 , pp. 179-186
    • Thornton, D.J.1
  • 7
    • 84856324259 scopus 로고    scopus 로고
    • Mass spectrometric analysis of mucin core proteins
    • Kesimer, M., & Sheehan, J. K. Mass spectrometric analysis of mucin core proteins. Methods Mol Biol. 842 67-79 (2012).
    • (2012) Methods Mol Biol , vol.842 , pp. 67-79
    • Kesimer, M.1    Sheehan, J.K.2
  • 8
    • 84915746080 scopus 로고    scopus 로고
    • Multiple enzyme approach for the characterization of glycan modifications on the Cterminus of the intestinal MUC2mucin
    • van der Post, S., Thomsson, K. A., & Hansson, G. C. Multiple enzyme approach for the characterization of glycan modifications on the Cterminus of the intestinal MUC2mucin. J Proteome Res. 13 (12), 6013-6023 (2014).
    • (2014) J Proteome Res , vol.13 , Issue.12 , pp. 6013-6023
    • Van Der Post, S.1    Thomsson, K.A.2    Hansson, G.C.3
  • 9
    • 0028310723 scopus 로고
    • Identification of glycoproteins on nitrocellulose membranes and gels
    • Thornton, D. J., Carlstedt, I., & Sheehan, J. K. Identification of glycoproteins on nitrocellulose membranes and gels. Methods Mol Biol. 32 119-128 (1994).
    • (1994) Methods Mol Biol , vol.32 , pp. 119-128
    • Thornton, D.J.1    Carlstedt, I.2    Sheehan, J.K.3
  • 10
    • 0030122080 scopus 로고    scopus 로고
    • Identification of glycoproteins on nitrocellulose membranes and gels
    • Thornton, D. J., Carlstedt, I., & Sheehan, J. K. Identification of glycoproteins on nitrocellulose membranes and gels. Mol Biotechnol. 5 (2), 171-176 (1996).
    • (1996) Mol Biotechnol , vol.5 , Issue.2 , pp. 171-176
    • Thornton, D.J.1    Carlstedt, I.2    Sheehan, J.K.3
  • 11
    • 0034176225 scopus 로고    scopus 로고
    • Physical characterization of the MUC5AC mucin: A highly oligomeric glycoprotein whether isolated from cell culture or in vivo from respiratory mucous secretions
    • Sheehan, J. K. et al. Physical characterization of the MUC5AC mucin: a highly oligomeric glycoprotein whether isolated from cell culture or in vivo from respiratory mucous secretions. Biochem J. 347 Pt 1 37-44 (2000).
    • (2000) Biochem J , vol.347 , pp. 37-44
    • Sheehan, J.K.1
  • 12
    • 0031003594 scopus 로고    scopus 로고
    • Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule
    • Thornton, D. J., Howard, M., Khan, N., & Sheehan, J. K. Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule. J Biol Chem. 272 (14), 9561-9566 (1997).
    • (1997) J Biol Chem , vol.272 , Issue.14 , pp. 9561-9566
    • Thornton, D.J.1    Howard, M.2    Khan, N.3    Sheehan, J.K.4
  • 13
    • 2442454780 scopus 로고    scopus 로고
    • Identification of molecular intermediates in the assembly pathway of the MUC5AC mucin
    • Sheehan, J. K. et al. Identification of molecular intermediates in the assembly pathway of the MUC5AC mucin. J Biol Chem. 279 (15), 15698-15705 (2004).
    • (2004) J Biol Chem , vol.279 , Issue.15 , pp. 15698-15705
    • Sheehan, J.K.1
  • 14
    • 84867345602 scopus 로고    scopus 로고
    • Overexpressing mouse model demonstrates the protective role of Muc5ac in the lungs
    • Ehre, C. et al. Overexpressing mouse model demonstrates the protective role of Muc5ac in the lungs. Proc Natl Acad Sci U S A. 109 (41), 16528-16533 (2012).
    • (2012) Proc Natl Acad Sci U S A , vol.109 , Issue.41 , pp. 16528-16533
    • Ehre, C.1
  • 15
    • 64249168940 scopus 로고    scopus 로고
    • Airway and lung pathology due to mucosal surface dehydration in {beta}-epithelial Na+ channel-overexpressing mice: Role of TNF-{alpha} and IL-4R{alpha} signaling, influence of neonatal development, and limited efficacy of glucocorticoid treatment
    • Livraghi, A. et al. Airway and lung pathology due to mucosal surface dehydration in {beta}-epithelial Na+ channel-overexpressing mice: role of TNF-{alpha} and IL-4R{alpha} signaling, influence of neonatal development, and limited efficacy of glucocorticoid treatment. J Immunol. 182 (7), 4357-4367 (2009).
    • (2009) J Immunol , vol.182 , Issue.7 , pp. 4357-4367
    • Livraghi, A.1
  • 16
    • 84876356353 scopus 로고    scopus 로고
    • The ER stress transducer IRE1beta is required for airway epithelial mucin production
    • Martino, M. B. et al. The ER stress transducer IRE1beta is required for airway epithelial mucin production. Mucosal Immunol. 6 (3), 639-654 (2013).
    • (2013) Mucosal Immunol , vol.6 , Issue.3 , pp. 639-654
    • Martino, M.B.1
  • 17
    • 40649086036 scopus 로고    scopus 로고
    • Chronic exposure to beta-blockers attenuates inflammation and mucin content in a murine asthma model
    • Nguyen, L. P. et al. Chronic exposure to beta-blockers attenuates inflammation and mucin content in a murine asthma model. Am J Respir Cell Mol Biol. 38 (3), 256-262 (2008).
    • (2008) Am J Respir Cell Mol Biol , vol.38 , Issue.3 , pp. 256-262
    • Nguyen, L.P.1
  • 18
    • 84949032170 scopus 로고    scopus 로고
    • COPD exacerbations are associated with pro-inflammatory degradation of hyaluronic acid
    • Papakonstantinou, E. et al. COPD exacerbations are associated with pro-inflammatory degradation of hyaluronic acid. Chest. (2015).
    • (2015) Chest
    • Papakonstantinou, E.1
  • 19
    • 84856346919 scopus 로고    scopus 로고
    • Studying mucin secretion from human bronchial epithelial cell primary cultures
    • Abdullah, L. H., Wolber, C., Kesimer, M., Sheehan, J. K., & Davis, C. W. Studying mucin secretion from human bronchial epithelial cell primary cultures. Methods Mol Biol. 842 259-277 (2012).
    • (2012) Methods Mol Biol , vol.842 , pp. 259-277
    • Abdullah, L.H.1    Wolber, C.2    Kesimer, M.3    Sheehan, J.K.4    Davis, C.W.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.