Identification of Glycoproteins on Nitrocellulose Membranes and Gels

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 5, Issue 2, 1996, Pages 171-176

  Thornton, David J ^a   Carlstedt, Ingemar ^a   Sheehan, John K ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Alcian blue; Glycoprotein; High iron diamine; Lectins; Mucins; Periodic acid Schiff

Indexed keywords

ACRYLIC ACID RESIN; GLYCOPROTEIN; POLYACRYLAMIDE GELS; PYROXYLIN; SEPHAROSE;

AGAR GEL ELECTROPHORESIS; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL; PERIODIC ACID SCHIFF STAIN; POLYACRYLAMIDE GEL ELECTROPHORESIS; STAINING;

ACRYLIC RESINS; COLLODION; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GELS; GLYCOPROTEINS; PERIODIC ACID-SCHIFF REACTION; SEPHAROSE; STAINING AND LABELING;

EID: 0030122080     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02789065     Document Type: Article

References (10)

1. Carlstedt, I. and Sheehan, J. K. (1989) Structure and properties of cervical mucins. In mucus and related topics. SEB Symp. XLIII, 289-316.
2. Goa, J. (1955) Determination of protein-bound hexose. Scand. J. Clin. Invest. Suppl. 7(22), 19-25.
3. Lohmander, L. S., Deluca, S., Nilsson, B., Hascall, V. C., Caputo, C. B., Kimura, J., and Heinegård, D. (1980) The sialic acids: XI A periodate/resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J. Biol. Chem. 255, 6084-6091.
4. Mantle, M. and Allen, A. (1978) A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain. Biochem. Soc. Trans. 6, 607-609.
5. Thornton, D. J., Holmes, D. F., Sheehan, J. K., and Carlstedt, I. (1989) Quantitation of mucus glycoproteins blotted onto nitrocellulose membranes. Anal. Biochem. 182, 160-164.
6. Sheehan, J. K., Thornton, D. J., and Carlstedt, I. (1990) Histochemical methods used in biochemical approaches to mucus glycoproteins. Acta Histochemica, Suppl.-Band XL S. 133-135.
7. Thornton, D. J., Sheehan, J. K., and Carlstedt, I. (1991) Heterogeneity of mucus glycoproteins from cystic fibrotic sputum. Biochem. J. 276, 677-682.
8. Lev, R. and Spicer, S. S. (1965) A histochemical comparison of human epithelial mucins in hypersecretory states including pancreatic cystic fibrosis. Am. J. Pathol. 46, 23-47.
9. Fairbanks, G., Steck, T. L., and Wallach, D. F. M. (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10, 2606-2617.
10. Maciewicz, R. A. and Knight, P. J. (1988) Transmission densitometry of stained nitrocellulose paper. Anal. Biochem. 175, 85-90.