Multicenter quality assessment of 16S ribosomal DNA-sequencing for microbiome analyses reveals high inter-center variability

International Journal of Medical Microbiology

Volumn 306, Issue 5, 2016, Pages 334-342

  Hiergeist, Andreas ^a   Reischl, Udo ^a   Gessner, Andrè ^a   Garzetti, Debora ^b,c   Stecher, Bärbel ^b,c   Gálvez, Eric JC ^d   Strowig, Till ^d   Yang, Ines ^e   Suerbaum, Sebastian ^e   Fischer, Nicole ^f   Grundhoff, Adam ^f   Kashofer, Karl ^g   Thüringer, Andrea ^g   Baines, John F ^h,i   Vallier, Marie ^h,i   Clavel, Thomas ^j   Haller, Dirk ^j  

^a UNIVERSITY HOSPITAL REGENSBURG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c GermanCenter of Infection Research   (Germany)

^d HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^e HANNOVER MEDICAL SCHOOL   (Germany)

^f LEIBNIZ INSTITUTE FOR EXPERIMENTAL VIROLOGY   (Germany)

^g MEDICAL UNIVERSITY OF GRAZ   (Austria)

^h MAX PLANCK INSTITUTE FOR EVOLUTIONARY BIOLOGY   (Germany)

ⁱ UNIVERSITY OF KIEL   (Germany)

^j TECHNICAL UNIVERSITY OF MUNICH   (Germany)

more..

Author keywords

16S rDNA; External quality assessment; Microbiome; Next generation sequencing; Proficiency testing

Indexed keywords

BACTERIAL DNA; DNA 16S; RNA 16S; RIBOSOME DNA;

ACIDAMINOCOCCACEAE; ALICYCLOBACILLUS; AMPLICON; ARTICLE; BACTEROIDACEAE; BIOINFORMATICS; COMMUNITY STRUCTURE; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; DNA SEQUENCE; FECES ANALYSIS; GENE SEQUENCE; HUMAN; MICROBIAL COMMUNITY; MICROBIOME; MICROFLORA; NONHUMAN; PEPTOSTREPTOCOCCACEAE; POLYMERASE CHAIN REACTION; PREVOTELLA; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RHIZOBIUM; WORKFLOW; CHEMISTRY; CLUSTER ANALYSIS; COMPARATIVE STUDY; GENETICS; GOOD LABORATORY PRACTICE; PHYLOGENY; PROCEDURES; REPRODUCIBILITY;

CLUSTER ANALYSIS; DNA, RIBOSOMAL; HUMANS; LABORATORY PROFICIENCY TESTING; MICROBIOTA; PHYLOGENY; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

EID: 84977139246     PISSN: 14384221     EISSN: 16180607     Source Type: Journal    
DOI: 10.1016/j.ijmm.2016.03.005     Document Type: Article

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