Primer and platform effects on 16S rRNA tag sequencing

Frontiers in Microbiology

Volumn 6, Issue AUG, 2015, Pages

  Tremblay, Julien ^a,b   Singh, Kanwar ^a   Fern, Alison ^a   Kirton, Edward S ^a   He, Shaomei ^a   Woyke, Tanja ^a   Lee, Janey ^a   Chen, Feng ^c   Dangl, Jeffery L ^d   Tringe, Susannah G ^a  

^a DOE JOINT GENOME INSTITUTE   (United States)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c ILLUMINA INC   (United States)

^d UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

16S rRNA gene sequencing; Amplification; Community assembly; High throughput sequencing; Microbial diversity; Microbial population and community ecology; Sequencing error

Indexed keywords

DNA 16S; RNA 16S;

ARTICLE; GENE AMPLIFICATION; GENE CLUSTER; GENE DOSAGE; METAGENOMICS; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SPECIES DIVERSITY;

EID: 84940984953     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2015.00771     Document Type: Article

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