Solubilization and Iterative Saturation Mutagenesis of α1,3-fucosyltransferase from Helicobacter pylori to enhance its catalytic efficiency

Biotechnology and Bioengineering

Volumn 113, Issue 8, 2016, Pages 1666-1675

  Choi, Yun Hee ^a   Kim, Jong Hoon ^a   Park, Bum Seok ^a   Kim, Byung Gee ^a  

^a Seoul National University   (South Korea)

Author keywords

fucosyllactose; fucosyltransferase; human milk oligosaccharide; iterative saturation mutagenesis; protein engineering

Indexed keywords

BINDING ENERGY; BINDING SITES; BINS; BIOCHEMISTRY; ENZYME ACTIVITY; ESCHERICHIA COLI; MUTAGENESIS; OLIGOSACCHARIDES; PROTEINS; SACCHARIFICATION; SOLUBILITY; SUGARS;

FUCOSYLLACTOSE; FUCOSYLTRANSFERASES; HUMAN MILK OLIGOSACCHARIDES; PROTEIN ENGINEERING; SATURATION MUTAGENESIS;

CATALYST ACTIVITY;

ALPHA 1,3 FUCOSYLTRANSFERASE; GUANOSINE DIPHOSPHATE FUCOSE; LACTOSE;

ARTICLE; BINDING SITE; CATALYSIS; CATALYTIC EFFICIENCY; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SYNTHESIS; HELICOBACTER PYLORI; IN VITRO STUDY; MOLECULAR DOCKING; MUTAGENESIS; NONHUMAN; PROTEIN EXPRESSION; SOLUBILITY; SOLUBILIZATION;

EID: 84977138183     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25944     Document Type: Article

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