DNA immunization combined with scFv phage display identifies antagonistic GCGR specific antibodies and reveals new epitopes on the small extracellular loops

mAbs

Volumn 8, Issue 6, 2016, Pages 1126-1135

  van der Woning, Bas ^a   De Boeck, Gitte ^a   Blanchetot, Christophe ^a   Bobkov, Vladimir ^a,b   Klarenbeek, Alex ^c   Saunders, Michael ^a   Waelbroeck, Magali ^d   Laeremans, Toon ^e   Steyaert, Jan ^e,f   Hultberg, Anna ^a   De Haard, Hans ^a  

^a ArGEN X BVBA   (Belgium)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

^c UTRECHT UNIVERSITY   (Netherlands)

^d UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^e Confotherapeutics ^*  (Belgium)

^f DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

DNA immunizations; glucagon receptor; llama; phage display; single chain Fv; virus like particles

Indexed keywords

CHEMOKINE RECEPTOR CXCR4; EPITOPE; G PROTEIN COUPLED RECEPTOR; GLUCAGON RECEPTOR ANTAGONIST; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; VIRUS LIKE PARTICLE VACCINE; CATHELICIDIN; CATHELICIDIN ANTIMICROBIAL PEPTIDE; GLUCAGON RECEPTOR; IMMUNOGLOBULIN F(AB) FRAGMENT; MEMBRANE PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; CAMP ASSAY; CHEMICAL BINDING; CONTROLLED STUDY; DNA IMMUNIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENETIC SELECTION; GENETIC TRANSFECTION; HEAVY CHAIN; HUMAN; HUMAN CELL; IMMUNE RESPONSE; LLAMA; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SURFACE PLASMON RESONANCE; VIRUS LIKE AGENT; ANIMAL; ANTAGONISTS AND INHIBITORS; ANTIBODY SPECIFICITY; BLOOD; CELL CULTURE; CELL SURFACE DISPLAY; CHO CELL LINE; CRICETULUS; FIBROBLAST; GENETICS; HEK293 CELL LINE; IMMUNIZATION; IMMUNOLOGY; ISOLATION AND PURIFICATION; NEW WORLD CAMELID; PLASMID;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CAMELIDS, NEW WORLD; CATHELICIDINS; CELL SURFACE DISPLAY TECHNIQUES; CELLS, CULTURED; CHO CELLS; CRICETULUS; FIBROBLASTS; HEK293 CELLS; HUMANS; IMMUNIZATION; IMMUNODOMINANT EPITOPES; IMMUNOGLOBULIN FAB FRAGMENTS; MEMBRANE PROTEINS; PLASMIDS; RECEPTORS, GLUCAGON; SINGLE-CHAIN ANTIBODIES; VACCINES, VIRUS-LIKE PARTICLE;

EID: 84976286576     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.1080/19420862.2016.1189050     Document Type: Article

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